Abscisic acid—lipid interactions: a phospholipid monolayer study

Bürner, Hans; Benz, Roland; Gimmler, H.; Hartung, Wolfram; Stillwell, William

doi:10.1016/0005-2736(93)90086-f

Cited by 19 publications

(15 citation statements)

References 30 publications

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“…3) suggesting that the undissociated ABA rather than the anion was causing the eect. The anion does not penetrate membranes (BuÈ rner et al 1993). …”

Section: Resultsmentioning

confidence: 98%

“…A simple interaction of ABA with the lipid-phase of the plasmalemma (BuÈ rner et al 1993) is very unlikely to be the reason for the increased Lp. Concentrations of ABA sucient to enhance membrane permeability to cations, anions and neutral solutes are in the millimolar range (more references are cited in BuÈ rner et al 1993), which are higher by a factor of 1´10 3 ±1´10 4 of the ABA-concentrations used in this study.…”

Section: Discussionmentioning

confidence: 97%

“…The permeability of the protonated ABA has been shown to be much larger than that of the anion (Slovik et al 1995). BuÈ rner et al (1993) have shown that the deprotonated ABA anion, as it is present at pH 8.0 nearly exclusively, cannot penetrate arti®cial phospholipid membranes.…”

Section: Discussionmentioning

confidence: 98%

“…At pH 8.0, on the other hand, more than 99.9% of ABA was present in its charged form (anion). It is known that, in the latter case, the permeability of ABA through cell membranes is quite low (BuÈ rner et al 1993). Cell Lp was measured in 11-d-old roots that were incubated in nutrient solution containing 100 nM ABA at the two pH values 5.5 and 8.0 (10 mM Hepes-KOH) for 30 min, 1 h and 2 h. Control roots were incubated for 1 h in nutrient solution at a pH of 5.5 without ABA.…”

Section: Hydrostatic Experimentsmentioning

confidence: 99%

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Abscisic acid and hydraulic conductivity of maize roots: a study using cell- and root-pressure probes

Hose

Steudle

Hartung

2000

Planta

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254

179

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Using root- and cell-pressure probes, the effects of the stress hormone abscisic acid (ABA) on the water-transport properties of maize roots (Zea mays L.) were examined in order to work out dose and time responses for root hydraulic conductivity. Abscisic acid applied at concentrations of 100-1,000 nM increased the hydraulic conductivity of excised maize roots both at the organ (root Lp(r): factor of 3 4) and the root cell level (cell Lp: factor of 7-27). Effects on the root cortical cells were more pronounced than at the organ level. From the results it was concluded that ABA acts at the plasmalemma, presumably by an interaction with water channels. Abscisic acid therefore facilitated the cell-to-cell component of transport of water across the root cylinder. Effects on cell Lp were transient and highly specific for the undissociated (+)-cis-trans-ABA. The stress hormone ABA facilitates water uptake into roots as soils start drying, especially under non-transpiring conditions, when the apoplastic path of water transport is largely excluded.

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“…3) suggesting that the undissociated ABA rather than the anion was causing the eect. The anion does not penetrate membranes (BuÈ rner et al 1993). …”

Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

Section: Hydrostatic Experimentsmentioning

confidence: 99%

See 2 more Smart Citations

Abscisic acid and hydraulic conductivity of maize roots: a study using cell- and root-pressure probes

Hose

Steudle

Hartung

2000

Planta

Self Cite

254

179

View full text Add to dashboard Cite

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“…The studies on artificial membrane systems proved that IAA molecules change permeability of phosphatidylcholine bilayer [21,22]. The membrane disturbing effect was also evidenced for the other natural plant hormone, Abscisic acid (ABA) -in this case it was evidenced that the influence of ABA on membrane organisation depends on the model system composition [22] and the membrane organisation was suggested as a factor determining the effect of auxin [22,23]. It is clear that the mechanism of auxins toxicity to both plants and animals is highly complex and involves the number of biochemical changes induced by these compounds at the level of cell and membrane.…”

Section: Introductionmentioning

confidence: 91%

The studies on the toxicity mechanism of environmentally hazardous natural (IAA) and synthetic (NAA) auxin – The experiments on model Arabidopsis thaliana and rat liver plasma membranes

Hąc-Wydro

Flasiński

2015

Colloids and Surfaces B: Biointerfaces

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Triacontanol and Jasmonic Acid Differentially Modulate the Lipid Organization as Evidenced by the Fluorescent Probe Behavior and 31P Nuclear Magnetic Resonance Shifts in Model Membranes

et al. 2009

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Fluorescence resonance energy transfer (FRET), time-resolved fluorescence and anisotropy decays were determined in large unilamellar vesicles (LUVs) of egg phosphatidylcholine with the FRET pair N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dipalmitoyl-sn-glycero-3-phospho-ethanolamine as donor and lissamine rhodamine B 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine as acceptor, using 2-ps pulses from a Ti:sapphire laser on LUVs with incorporated plant growth regulators: triacontanol (TRIA) and jasmonic acid (JA). FRET efficiency, energy transfer rate, rotation correlation time, microviscosity, and diffusion coefficient of lateral diffusion of lipids were calculated from these results. It was observed that TRIA and JA differentially modulated all parameters studied. The effect of JA in such modulations was always partially reversed by TRIA. Also, the generalized polarization of laurdan fluorescence indicated that JA enhances the degree of hydration in lipid bilayers to a larger extent than does TRIA. Solid-state (31)P magic-angle spinning nuclear magnetic resonance spectra of LUVs showed two chemical shifts, at 0.009 and -11.988 ppm, at low temperatures (20 degrees C), while at increasing temperatures (20-60 degrees C) only one (at -11.988 ppm) was prominent and the other (0.009 ppm) gradually became obscure. However, LUVs with TRIA exhibited only one of the shifts at 0.353 ppm even at lower temperatures and JA did not affect the chemical shifts.

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Abscisic acid—lipid interactions: a phospholipid monolayer study

Cited by 19 publications

References 30 publications

Abscisic acid and hydraulic conductivity of maize roots: a study using cell- and root-pressure probes

Abscisic acid and hydraulic conductivity of maize roots: a study using cell- and root-pressure probes

The studies on the toxicity mechanism of environmentally hazardous natural (IAA) and synthetic (NAA) auxin – The experiments on model Arabidopsis thaliana and rat liver plasma membranes

Triacontanol and Jasmonic Acid Differentially Modulate the Lipid Organization as Evidenced by the Fluorescent Probe Behavior and 31P Nuclear Magnetic Resonance Shifts in Model Membranes

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