Abscisic acid negatively regulates the Polycomb‐mediated H3K27me3 through the PHD‐finger protein, VIL1

Zong, Wei; Kim, Junghyun; Bordiya, Yogendra; Qiao, Hong; Sung, Sibum

doi:10.1111/nph.18156

Cited by 18 publications

(4 citation statements)

References 60 publications

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“…4g ). Interestingly however, when we compared our vrn2-5 up DEGs with two previously published vil1 mutant RNA seq data sets (Kim et al, 2023, Zong et al, 2022), we saw very little overlap (only 3 genes, and a complete absence of SAURs and HAT4 etc) as well as an absence of GO terms linked “red/far red” or “shade avoidance” response ( Fig. S4c ).…”

Section: Resultsmentioning

confidence: 80%

VRN2-PRC2 facilitates light-triggered repression of PIF signaling to coordinate growth in Arabidopsis

Osborne,

Labandera,

Ryder

et al. 2024

Preprint

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The polycomb protein VERNALIZATION2 (VRN2) is a plant-specific subunit of the polycomb repressive complex 2 (PRC2), a conserved eukaryotic holoenzyme that represses gene expression by depositing the histone H3K27me3 mark in chromatin. Previous work established VRN2 as an oxygen-regulated target of the N-degron pathway that may function as a sensor subunit connecting PRC2 activity to the perception of positional and environmental cues. Here we show that VRN2 is enriched in hypoxic meristematic regions and emerging leaves of Arabidopsis under non-stressed conditions, and that vrn2 mutants are larger than wild type, indicating that VRN2-PRC2 negatively regulates growth and development. This growth phenotype is caused by ectopic expression of genes that promote cell expansion, including many SAUR genes and other direct PIF transcription factor targets. Analysis of SAUR19 promoter activity and expression dynamics revealed that VRN2 is required to specifically repress these genes in the light. Moreover, we show that VRN2 is epistatic to PIF4, and directly binds and methylates histones of key loci in the PIF4 transcriptional network to provide robust light-responsive control of gene expression and growth. We propose that hypoxia-stabilised VRN2-PRC2 sets a conditionally repressed chromatin state at PIF-regulated hub genes early in leaf ontogeny coinciding with the cell division phase, and that this is required for enhancing their subsequent repression via a light-responsive signalling cascade as cells enter the expansion phase. Thus, we have identified VRN2-PRC2 as core component of a spatially regulated and developmentally encoded epigenetic mechanism that co-ordinates environment-responsive growth by facilitating light-triggered suppression of PIF signalling.

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Section: Resultsmentioning

confidence: 80%

VRN2-PRC2 facilitates light-triggered repression of PIF signaling to coordinate growth in Arabidopsis

Osborne,

Labandera,

Ryder

et al. 2024

Preprint

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“…Histone deacetylase HDA9 has also been suggested to be required for the PRC2 activity in the repression of FLC 40 . Notably VRN5 and PRC2 and HDAC are required for repression of the ABA response in seedlings 32 , 41 , 42 . However, a lack of VEL3 expression at this stage confines VEL3 to the control of primary seed dormancy, which is established during seed development.…”

Section: Discussionmentioning

confidence: 99%

A VEL3 histone deacetylase complex establishes a maternal epigenetic state controlling progeny seed dormancy

et al. 2023

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Mother plants play an important role in the control of dormancy and dispersal characters of their progeny. In Arabidopsis seed dormancy is imposed by the embryo-surrounding tissues of the endosperm and seed coat. Here we show that VERNALIZATION5/VIN3-LIKE 3 (VEL3) maintains maternal control over progeny seed dormancy by establishing an epigenetic state in the central cell that primes the depth of primary seed dormancy later established during seed maturation. VEL3 colocalises with MSI1 in the nucleolus and associates with a histone deacetylase complex. Furthermore, VEL3 preferentially associates with pericentromeric chromatin and is required for deacetylation and H3K27me3 deposition established in the central cell. The epigenetic state established by maternal VEL3 is retained in mature seeds, and controls seed dormancy in part through repression of programmed cell death-associated gene ORE1. Our data demonstrates a mechanism by which maternal control of progeny seed physiology persists post-shedding, maintaining parental control of seed behaviour.

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“…The pathogen- and drought-inducible SlSDG33 and SlSDG34 , which are homologous to Arabidopsis SDG8 , negatively regulate drought tolerance by promoting the H3K4me3 and H3K36me3 deposition on a set of genes with negative regulatory roles in stress tolerance. SlSDG33 and SlSDG35 might act additively in drought response as the double mutant showed superior tolerance to the single mutants ( Zong et al, 2022 ). During the postharvest desiccation process in tea plants, the ABA-biosynthesis genes are activated with elevated histone acetylation and decreased H3K9me2, leading to ABA accumulation ( Gu et al, 2021 ).…”

Section: Two Dynamic Counterparts In Stress-responsive Gene Transcrip...mentioning

confidence: 99%

“…Further, VIL1 recruits PRC2 complex to trimethylate H3K27me3 and repress the transcription of ABI3/4/5. Loss-of-VIL1 leads to hypersensitivity to ABA and enhanced drought tolerance ( Zong et al, 2022 ). GmPHD6 (PLANT HOMEODOMAIN 6) interacts with the GmLHP1/2 coactivators through the PHD domain to form a transcriptional activation complex.…”

Section: Cross-talks Of Histone (De)methylation In Response To Osmoti...mentioning

confidence: 99%

Methylation hallmarks on the histone tail as a linker of osmotic stress and gene transcription

2022

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Plants dynamically manipulate their gene expression in acclimation to the challenging environment. Hereinto, the histone methylation tunes the gene transcription via modulation of the chromatin accessibility to transcription machinery. Osmotic stress, which is caused by water deprivation or high concentration of ions, can trigger remarkable changes in histone methylation landscape and genome-wide reprogramming of transcription. However, the dynamic regulation of genes, especially how stress-inducible genes are timely epi-regulated by histone methylation remains largely unclear. In this review, recent findings on the interaction between histone (de)methylation and osmotic stress were summarized, with emphasis on the effects on histone methylation profiles imposed by stress and how histone methylation works to optimize the performance of plants under stress.

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Abscisic acid negatively regulates the Polycomb‐mediated H3K27me3 through the PHD‐finger protein, VIL1

Cited by 18 publications

References 60 publications

VRN2-PRC2 facilitates light-triggered repression of PIF signaling to coordinate growth in Arabidopsis

VRN2-PRC2 facilitates light-triggered repression of PIF signaling to coordinate growth in Arabidopsis

A VEL3 histone deacetylase complex establishes a maternal epigenetic state controlling progeny seed dormancy

Methylation hallmarks on the histone tail as a linker of osmotic stress and gene transcription

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