Abscisic acid promotes shoot regeneration in Arabidopsis zygotic embryo explants

Paulraj, Subramanian; López‐Villalobos, Arturo; Yeung, Edward C.

doi:10.1007/s11627-014-9624-2

Cited by 7 publications

(9 citation statements)

References 31 publications

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“…The experiments were performed using Arabidopsis thaliana ecotype Landsberg erecta as detailed in Paulraj et al (2014). In brief, siliques containing immature zygotic embryos at the cotyledonary stage were collected from 6-to 8-week old plants.…”

Section: Methodsmentioning

confidence: 99%

“…The tissue culture procedures were detailed as in Paulraj et al (2014). The embryos were grown on the callus induction medium (CIM) consisting of Gamborg's B5 salts (Gamborg et al 1968) and vitamins, 2% sucrose, 4.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D) (Sigma-Aldrich, St Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

“…At the time of culture, the bent cotyledon stage embryo had a defined embryo axis and two cotyledons (Paulraj et al 2014). Distinct tissue patterns were present at this time.…”

Section: Callus Formation From Embryo Explantsmentioning

confidence: 95%

“…An efficient shoot organogenesis protocol for Arabidopsis zygotic embryo explants of Landsberg erecta ecotype has recently been established (Paulraj et al 2014). The Landsberg erecta ecotype was chosen because it has been shown to have a high regeneration potential in both root and leaf explant cultures and the widely used Columbia (COL) ecotype was proven as highly recalcitrant (Feldmann and Marks 1986;Valvekens et al 1988;Candela et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…The appearance of darker green organogenic calluses was associated with the accumulation of anthocyanins. Hence, the organogenic callus tissues in the SIM and the SDM were easily identified by their color (Paulraj et al 2014). The ease of organogenic callus identification presents an ideal system to study the process of shoot organogenesis as we know the precise location where the shoot apical meristems (SAM) are to be formed.…”

Section: Introductionmentioning

confidence: 99%

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Shoot apical meristem ontogeny inArabidopsisembryo explants treated with abscisic acid

Paulraj

López‐Villalobos

Yeung

2015

Botany

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The formation of meristemoids and the ontogeny of the shoot apical meristem were studied using cultured zygotic embryos of Arabidopsis thaliana LER ecotype. In the callus induction treatment, the procambial cells within the cotyledons of the embryo explants proliferated and gave rise to callus tissues. At the end of the treatment, a band of small cytoplasmic-rich cells derived from the procambium was produced and located at the outer surface of the callus. Upon transfer to the shoot induction medium (SIM) in the absence of abscisic acid (ABA), the cytoplasmic cells differentiated mainly into vascular elements and vacuolated parenchyma cells. This pattern of development negatively affected the explants ability to produce meristemoids and shoot apical meristems (SAMs). Contrary to the control, the inclusion of ABA in the SIM resulted first in starch synthesis and accumulation in the surface cytoplasmic cells. This was followed by the formation of cytoplasmic cells among the starch-rich cells; further proliferation of the cytoplasmic cells resulted in the formation of meristemoids. The formation of tracheary elements was suppressed in the ABA-containing SIM. Upon transferring to the shoot development medium which lacked plant growth regulators, some meristemoids differentiated into apical meristem cells. These cells had distinct nuclei and nucleoli, with little starch present.Additional cell divisions increased the size of the future SAM. Shoot buds with distinct SAMs were clearly delineated with the appearance of leaf primordia.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…At the time of culture, the bent cotyledon stage embryo had a defined embryo axis and two cotyledons (Paulraj et al 2014). Distinct tissue patterns were present at this time.…”

Section: Callus Formation From Embryo Explantsmentioning

confidence: 95%