Abscisic Acid Related Metabolites in Sweet Cherry Buds (Prunus avium L.)

Fm, Chmielewski; Baldermann, Susanne; Kp, Götz; Homann, Thomas; Gödeke, Kristin; Schumacher, F. W.; Huschek, Gerd; Rawel, Harshadrai M.

doi:10.4172/2376-0354.1000221

Cited by 6 publications

(4 citation statements)

References 37 publications

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“…Furthermore, the mRNA level of MdABA8′H, which encodes an ABA 8′-hydroxylase enzyme involved in ABA catabolism [40], was clearly upregulated during endodormacy and strongly repressed in ecodormancy (Figure 5B). As MdABA8′H mRNA expression is induced by ABA [40], together with the MdNCED1 expression profile (Figure 5A), this result corroborates previous reports proposing that highest ABA levels are found during endodormancy and negatively correlates with budbreak in different fruit tree species, including apple, sour cherry, sweet cherry, pear and peach [12,37,41,42]. This result suggested that miR159a is induced by ABA during apple bud dormancy.…”

Section: The Aba Regulatory Module Mir159a-myb33/myb65 Is Conserved In Applesupporting

confidence: 90%

“…The plant hormone ABA is a central regulator of dormancy by mechanisms involving repression of cell cycle and intercellular communication via plasmodesmata [52]. In many fruit tree and vine species such as apple, peach, pear, sweet cherry and grapevine (Vitis vinifera), ABA levels increase during the endodormancy phase and decline towards budbreak [12,36,41,42,53,63]. We could confirm this pattern of ABA accumulation in two consecutive years through the mRNA level quantification of the ABA-responding gene MdABA8 H [40] (Figure 5B).…”

Section: A Possible Mechanism Of Aba Hyposensibilization Mediated By the Mdm-mir159a-myb33 Module During Ecodormancymentioning

confidence: 99%

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The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy

Garighan

Dvorak

Estevan

et al. 2021

Plants

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Winter dormancy is an adaptative mechanism that temperate and boreal trees have developed to protect their meristems against low temperatures. In apple trees (Malus domestica), cold temperatures induce bud dormancy at the end of summer/beginning of the fall. Apple buds stay dormant during winter until they are exposed to a period of cold, after which they can resume growth (budbreak) and initiate flowering in response to warmer temperatures in spring. It is well-known that small RNAs modulate temperature responses in many plant species, but however, how small RNAs are involved in genetic networks of temperature-mediated dormancy control in fruit tree species remains unclear. Here, we have made use of a recently developed ARGONAUTE (AGO)-purification technique to isolate small RNAs from apple buds. A small RNA-seq experiment resulted in the identification of 17 micro RNAs (miRNAs) that change their pattern of expression in apple buds during dormancy. Furthermore, the functional analysis of their predicted target genes suggests a main role of the 17 miRNAs in phenylpropanoid biosynthesis, gene regulation, plant development and growth, and response to stimulus. Finally, we studied the conservation of the Arabidopsis thaliana regulatory miR159-MYB module in apple in the context of the plant hormone abscisic acid homeostasis.

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Section: The Aba Regulatory Module Mir159a-myb33/myb65 Is Conserved In Applesupporting

confidence: 90%

Section: A Possible Mechanism Of Aba Hyposensibilization Mediated By the Mdm-mir159a-myb33 Module During Ecodormancymentioning

confidence: 99%

The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy

Garighan

Dvorak

Estevan

et al. 2021

Plants

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“…For example, in Prunus mume, ABA contents were high at the beginning of ED, and progressively decreased until DR [12]. Similar results were reported in peach [53] and in some other woody species such as pear [52,54] and sweet cherry [55]. Some works determined that ABA is only important for bud establishment and maintenance, but not for DR [5].…”

Section: Plant Hormonessupporting

confidence: 72%

Interplay among Antioxidant System, Hormone Profile and Carbohydrate Metabolism during Bud Dormancy Breaking in a High-Chill Peach Variety

et al. 2021

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(1) Background: Prunus species have the ability to suspend (induce dormancy) and restart growth, in an intricate process in which environmental and physiological factors interact. (2) Methods: In this work, we studied the evolution of sugars, antioxidant metabolism, and abscisic acid (ABA) and gibberellins (GAs) levels during bud dormancy evolution in a high-chill peach variety, grown for two seasons in two different geographical areas with different annual media temperature, a cold (CA) and a temperate area (TA). (3) Results: In both areas, starch content reached a peak at ecodormancy, and then decreased at dormancy release (DR). Sorbitol and sucrose declined at DR, mainly in the CA. In contrast, glucose and fructose levels progressively rose until DR. A decline in ascorbate peroxidase, dehydroascorbate reductase, superoxide dismutase and catalase activities occurred in both seasons at DR. Moreover, the H2O2-sensitive SOD isoenzymes, Fe-SOD and Cu,Zn-SOD, and two novel peroxidase isoenzymes, were detected. Overall, these results suggest the occurrence of a controlled oxidative stress during DR. GA7 was the major bioactive GA in both areas, the evolution of its levels being different between seasons and areas. In contrast, ABA content decreased during the dormancy period in both areas, resulting in a reduction in the ABA/total GAs ratio, being more evident in the CA. (4) Conclusion: A possible interaction sugars-hormones-ROS could take place in high-chill peach buds, favoring the DR process, suggesting that, in addition to sugar metabolism, redox interactions can govern bud DR, regardless of chilling requirements.

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“…Models can be improved with dormancy break observations, but to date, only a limited number of controlled experiments on fruit and nut trees allowed for calibrating of models against dormancy phase change observations [6,18,52,57]. New research is emerging using experimental indicators such as water content or metabolites such as abscisic acids or sugars to assess the dormancy state [58][59][60], but the number of years of observations is still too low for long-term analyses, so alternative techniques like the PLSR are preferred. In the absence of local field experiments to determine dormancy break, phenological models can only provide indications of phenological trends.…”

Section: Evaluation Of the Phenological Modelsmentioning

confidence: 99%

Comparing Apple and Pear Phenology and Model Performance: What Seven Decades of Observations Reveal

Drepper

Gobin

Remy³

et al. 2020

Agronomy

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Based on observations for the beginning of the flowering stage of Malus domestica (apple) and Pyrus communis (pear) for the 1950-2018 period, phenological trends in north-eastern Belgium were investigated in function of temperatures during dormancy. Moreover, two different phenological models were adapted and evaluated. Median flowering dates of apple were on average 9.5 days earlier following warm dormancy periods, and 11.5 days for pear, but the relationship between bloom date and temperature was found not to be linear, suggesting delayed fulfilment of dormancy requirements due to increased temperatures during the chilling period. After warm chilling periods, an average delay of 5.0 and 10.6 days in the occurrence date of dormancy break was predicted by the phenological models while the PLSR reveals mixed signals regarding the beginning of flowering. Our results suggest overlapping chilling and forcing processes in a transition phase. Regarding the beginning of flowering, a dynamic chill model coupled to a growing degree days estimation yielded significantly lower prediction errors (on average 5.0 days) than a continuous chill-forcing model (6.0 days), at 99% confidence level. Model performance was sensitive to the applied parametrization method and limitations for the application of both models outside the past temperature ranges became apparent.Other weather-related hazards were several light frost nights, frequent local hailstorms, and three heatwaves in 2019, an extensive drought period for more than two months in combination with a heat wave in 2018, water excess and storms in 2016, and a devastating summer storm in 2011. The consequences of extreme weather in addition to the Russian embargo on fruit imports since 2014 led the entire Belgian fruit sector into an officially recognized crisis, to the extent that federal and regional governments are preparing policies to increase resilience in the face of more frequent extreme weather events and in the context of a changing climate in general.Therefore, a better understanding is needed of the impacts of warming on phenology in the specific case of apple and pear cultivation in Flanders. By means of adapted phenological models, variations in flowering and the related frost-sensitive stage can be estimated across temporal and spatial scales, which can support decision-making regarding (new) orchard locations and cultivar selection.Following [3], the part of the growth cycle between late summer and budburst is conceptualized as dormancy period, and can be decomposed in a sequence of paradormancy, endodormancy, and ecodormancy phases. Endodormancy, i.e., 'chilling period', is induced by lower temperatures in late fall. The moment that a species' specific exposure to cold temperatures has been fulfilled, ecodormancy is induced and the accumulation of growing degree hours becomes the determining process for further phenological development [4], i.e., 'forcing period'. From then onwards, warmer temperatures favor earlier and no longer later bloom. Insolation and tem...

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Abscisic Acid Related Metabolites in Sweet Cherry Buds (Prunus avium L.)

Cited by 6 publications

References 37 publications

The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy

The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy

Interplay among Antioxidant System, Hormone Profile and Carbohydrate Metabolism during Bud Dormancy Breaking in a High-Chill Peach Variety

Comparing Apple and Pear Phenology and Model Performance: What Seven Decades of Observations Reveal

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