Abscisic acid signaling activates distinct VND transcription factors to promote xylem differentiation in Arabidopsis

Ramachandran, Prashanth; Augstein, Frauke; Mazumdar, Shamik; Nguyen, Thanh Van; Minina, Elena A.; Melnyk, Charles W.; Carlsbecker, Annelie

doi:10.1016/j.cub.2021.04.057

Cited by 49 publications

(64 citation statements)

References 36 publications

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“…These complex developmental processes are extremely sensitive to signals sensing environmental changes ( Chaiwanon et al, 2016 ; Perini et al, 2012 ). For instance, recent findings ( Jang and Choi, 2018 ; Jang et al, 2017 ; Ramachandran et al, 2020 ; 2021 ) have revealed that xylem differentiation within the stele is largely influenced by reduced water availability via JA and ABA signaling.…”

Section: Introductionmentioning

confidence: 99%

The Danger-Associated Peptide PEP1 Directs Cellular Reprogramming in the Arabidopsis Root Vascular System

Dhar

Kim

Segonzac

et al. 2021

Molecules and Cells

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When perceiving microbe-associated molecular patterns (MAMPs) or plant-derived damage-associated molecular patterns (DAMPs), plants alter their root growth and development by displaying a reduction in the root length and the formation of root hairs and lateral roots. The exogenous application of a MAMP peptide, flg22, was shown to affect root growth by suppressing meristem activity. In addition to MAMPs, the DAMP peptide PEP1 suppresses root growth while also promoting root hair formation. However, the question of whether and how these elicitor peptides affect the development of the vascular system in the root has not been explored. The cellular receptors of PEP1, PEPR1 and PEPR2 are highly expressed in the root vascular system, while the receptors of flg22 ( FLS2 ) and elf18 ( EFR ) are not. Consistent with the expression patterns of PEP1 receptors, we found that exogenously applied PEP1 has a strong impact on the division of stele cells, leading to a reduction of these cells. We also observed the alteration in the number and organization of cells that differentiate into xylem vessels. These PEP1-mediated developmental changes appear to be linked to the blockage of symplastic connections triggered by PEP1. PEP1 dramatically disrupts the symplastic movement of free green fluorescence protein (GFP) from phloem sieve elements to neighboring cells in the root meristem, leading to the deposition of a high level of callose between cells. Taken together, our first survey of PEP1-mediated vascular tissue development provides new insights into the PEP1 function as a regulator of cellular reprogramming in the Arabidopsis root vascular system.

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Section: Introductionmentioning

confidence: 99%

The Danger-Associated Peptide PEP1 Directs Cellular Reprogramming in the Arabidopsis Root Vascular System

Dhar

Kim

Segonzac

et al. 2021

Molecules and Cells

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“…In addition, we found that ZjVND7 and ZjSMR1 were responsive to ABA with the same expression trend, implying a positive regulatory effect (Supplementary Figure 5). Moreover, the targeted ABRE sequence detected in autotetraploids indicated that the binding of ZjVND7 and ZjSMR1 may occur in response to the accumulation of antimitotic ABA and could further promote differences in vessel differentiation between diploid and autotetraploid plants (Ramachandran et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Direct Targets of ZjVND7 Reveals the Putative Roles of Whole-Genome Duplication in Sour Jujube in Regulating Xylem Vessel Differentiation and Drought Tolerance

Hou

Zhang

et al. 2022

Front. Plant Sci.

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The effects of whole-genome duplication span multiple levels. Previous study reported that the autotetraploid sour jujube exhibited superior drought tolerance than diploid. However, the difference in water transport system between diploids and autotetraploids and its mechanism remain unclear. Here, we found the number of xylem vessels and parenchyma cells in autotetraploid sour jujube increased to nearly twice that of diploid sour jujube, which may be closely related to the differences in xylem vessel differentiation-related ZjVND7 targets between the two ploidy types. Although the five enriched binding motifs are different, the most reliable motif in both diploid and autotetraploid sour jujube was CTTNAAG. Additionally, ZjVND7 targeted 236 and 321 genes in diploids and autotetraploids, respectively. More identified targeted genes of ZjVND7 were annotated to xylem development, secondary wall synthesis, cell death, cell division, and DNA endoreplication in autotetraploids than in diploids. SMR1 plays distinct roles in both proliferating and differentiated cells. Under drought stress, the binding signal of ZjVND7 to ZjSMR1 was stronger in autotetraploids than in diploids, and the fold-changes in the expression of ZjVND7 and ZjSMR1 were larger in the autotetraploids than in the diploids. These results suggested that the targeted regulation of ZjVND7 on ZjSMR1 may play valuable roles in autotetraploids in the response to drought stress. We hypothesized that the binding of ZjVND7 to ZjSMR1 might play a role in cell division and transdifferentiation from parenchyma cells to vessels in the xylem. This regulation could prolong the cell cycle and regulate endoreplication in response to drought stress and abscisic acid, which may be stronger in polyploids.

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“…Xylem gaps are not only observed upon salt stress, but certain genotypes display gaps also under normal conditions, including protoxylem gaps in ga4 (Fig. 3b) and metaxylem gaps upon inhibition of ABA signaling in the endodermis (Ramachandran et al, 2021).…”

Section: Expansins May Be Involved In Della Dependent Xylem Gap Forma...mentioning

confidence: 99%

“…The hormone abscisic acid (ABA) mediates these developmental responses by at least two different mechanisms. Firstly, ABA promotes the production of miR165 in the endodermis (Ramachandran et al, 2018; Bloch et al, 2019; Ramachandran et al, 2021). This miRNA moves into the stele to reduce homeodomain leucine zipper class III (HD-ZIP III) mRNA levels eventually leading to protoxylem formation in place of metaxylem (Carlsbecker et al, 2010; Miyashima et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Salinity induces discontinuous protoxylem by a DELLA-dependent mechanism promoting salt tolerance in Arabidopsis seedlings

Augstein

Carlsbecker

2022

Preprint

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Salinity is detrimental to plants and developmental adjustments limiting salt uptake and transport is therefore important for acclimation to high salt. These parameters may be influenced by xylem morphology, however, how plant root xylem development is affected by salt stress remains unclear. Through detailed phenotypic analyses, molecular and genetic techniques, we demonstrate that salt causes distinct effects on Arabidopsis seedling root xylem, and reveal underlying molecular mechanisms. Salinity causes intermittent inhibition of protoxylem cell differentiation, generating protoxylem gaps, in Arabidopsis and other eudicot seedlings. The extent of protoxylem gaps positively correlates with salt tolerance. Reduced gibberellin signaling is required for protoxylem gap formation. Mutant analyses reveal that the xylem differentiation regulator VASCULAR RELATED NAC DOMAIN 6 (VND6), along with secondary cell wall-producing and cell wall modifying enzymes, including EXPANSIN A1 (EXP1), are involved in protoxylem gap formation, in a DELLA-dependent manner. Salt stress impacts seedling survival and formation of protoxylem gaps is a means of enhancing salt tolerance. Salt stress likely reduces levels of bioactive gibberellins, stabilizing DELLAs, which in turn activate multiple factors modifying protoxylem differentiation. Formation of protoxylem gaps is induced in diverse eudicot species suggesting that this is an evolutionary conserved response for salt acclimation in seedlings.

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Abscisic acid signaling activates distinct VND transcription factors to promote xylem differentiation in Arabidopsis

Cited by 49 publications

References 36 publications

The Danger-Associated Peptide PEP1 Directs Cellular Reprogramming in the Arabidopsis Root Vascular System

The Danger-Associated Peptide PEP1 Directs Cellular Reprogramming in the Arabidopsis Root Vascular System

Genome-Wide Identification of Direct Targets of ZjVND7 Reveals the Putative Roles of Whole-Genome Duplication in Sour Jujube in Regulating Xylem Vessel Differentiation and Drought Tolerance

Salinity induces discontinuous protoxylem by a DELLA-dependent mechanism promoting salt tolerance in Arabidopsis seedlings

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