Absence of a direct role of phospholipid methylation in stimulus-secretion coupling and control of adenylate cyclase in guinea-pig and rat parotid gland

Padel, Ulrike; Unger, Clemens; Söling, Hans‐Dieter

doi:10.1042/bj2080205

Cited by 20 publications

(14 citation statements)

References 16 publications

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“…This result further suggests that methyltransferase activity and ligandreceptor interactions occur. Our results are similar to those of Padel et al [12] and Colard et al [29] in that basal and NaF-stimulated adenylate cyclase activity were not altered by a methyltransferase inhibitor. Contrary to our findings, Padel et al found that the activity of receptor-stimulated adenylate cyclase in isolated parotid microsomal membranes was not inhibited or stimulated by S-adenosylhomocysteine or by the addition of S-adenosylmethionine, and amylase secretion was not inhibited by inhibitors of methyltransferase.…”

Section: Discussionsupporting

confidence: 94%

Membrane Phospholipid Methylation is Associated with Surfactant Secretion in Rabbit Type II Alveolar Epithelial Cells

Kd¹,

Finkelstein²,

Dl³

1989

Experimental Lung Research

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We investigated the involvement of membrane phospholipid methylation in receptor-mediated secretion of surfactant in adult rabbit type II alveolar epithelial cells (type II pneumocyte). Phospholipid methyltransferase activity was found in type II pneumocyte microsomes. Cell cultures of adult rabbit type II pneumocytes were then used to assay methyltransferase activity in the presence of the beta-adrenergic agonist, terbutaline, and the methyltransferase inhibitor, 3-deazaadenosine. Terbutaline predictably stimulated adenylate cyclase activity and surfactant secretion. It was also found to stimulate incorporation of methyl groups into phosphatidylcholine and to increase beta-adrenergic receptor availability as assayed by binding of dihydroalprenolol (DHA). Surfactant secretion, as well as adenylate cyclase activity, were stimulated by terbutaline and were inhibited by 3-deazaadenosine. 3-Deazaadenosine did not inhibit DHA binding. These results suggest that phospholipid methylation plays a role in stimulus-secretion coupling in adult rabbit type II pneumocytes.

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Section: Discussionsupporting

confidence: 94%

Membrane Phospholipid Methylation is Associated with Surfactant Secretion in Rabbit Type II Alveolar Epithelial Cells

Kd¹,

Finkelstein²,

Dl³

1989

Experimental Lung Research

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“…Guinea pig parotid gland acini were prepared as described [5]. For subcellular fractionation the acinar cells were washed 3 times in medium A (130 mM KCI, 2 mM MgC12, 2 mM K phosphate, 1 mM dithiothreitol, 1 mM benzamidine, 20 mM Hepes-KOH, pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

“…Receptor-mediated release of calcium from intracellular pools by inositol 1,4,5-trisphosphate (Ins (1,4,5)P3) has been shown to occur in many different cell types. More recently, it has been reported that 5' -GTP can also stimulate the release of endogenous calcium.…”

Section: Introductionmentioning

confidence: 99%

Inositol 1,4,5‐trisphosphate and 5′‐GTP induce calcium release from different intracellular pools

1987

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It has been shown recently by several groups that 5'-GTP can release calcium from intraceilular compartments independently from inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) by a mechanism which seems to be different from that used by Ins(1,4,5)P 3. We report here for the first time that the 5'-GTP-sensitive and the Ins(l,4,5)P3-sensitive calcium pools reside in different intracellular compartments.

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“…the conversion of phosphatidylethanolamine into phosphatidylcholine (Hirata et al, 1979;Hirata & Axelrod, 1980). However, this is controversial since in different cell types it has been shown that phospholipid methylation is not directly related to adenylate cyclase activities (Colard & Breton, 1981;Padel et al, 1982) and is not ©D The Macmillan Press Ltd 1987 involved with whole cell activation (Randon et al, 1981;Schanche et al, 1982;Moore et al, 1982;Benyon et al, 1986). We previously demonstrated that platelet activation can be inhibited by the methylation inhibitors (Randon et al, 1981;, by mechanisms other than interference with phospholipid methylation, such as protein methylation (reviewed by O'Dea et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

Blockade by methylation inhibitors of the anaphylactic response of guinea‐pig lung strips

Randon

Lefort

Vargaftig

1987

British J Pharmacology

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The combination of two methylation inhibitors 3‐deazaadenosine (10−4 or 4 × 10−4 m) plus l‐homocysteine (2 × 10−4 m) caused a time‐dependent inhibition of antigen‐induced contraction, formation of thromboxane B2 (TxB2) and release of histamine from lung parenchyma strips taken from guinea‐pigs actively sensitized with ovalbumin (OA). The methylation inhibitors also prevented the lung strip contractions induced by the mediators platelet‐activating factor (Paf‐acether, 10−6 m), leukotriene D4 (LTD4, 10−8 and 3 × 10−8 m), and in part to arachidonic acid (10−6 and 10−5 m), under conditions where the contractions to histamine (10−6‐10−4 m) were virtually unaffected. TxB2 formation induced by these mediators or by OA was more affected by the methylation inhibitors than the lung strip contractions, indicating that prostaglandin formation is more sensitive to these inhibitors than the myotropic activity. In contrast, the suppressive effect of the methylation inhibitors on histamine secretion by parenchyma lung strips induced by OA followed the inhibition of the contraction. These results show that inhibitors of methyltransferases interfere with the myotropic responses and with the release of mediators by actively sensitized guinea‐pig lung strips stimulated with antigen, and suggest a major role for a methylation process in mediating the contraction of and mediator release by the lung parenchyma.

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Absence of a direct role of phospholipid methylation in stimulus-secretion coupling and control of adenylate cyclase in guinea-pig and rat parotid gland

Cited by 20 publications

References 16 publications

Membrane Phospholipid Methylation is Associated with Surfactant Secretion in Rabbit Type II Alveolar Epithelial Cells

Membrane Phospholipid Methylation is Associated with Surfactant Secretion in Rabbit Type II Alveolar Epithelial Cells

Inositol 1,4,5‐trisphosphate and 5′‐GTP induce calcium release from different intracellular pools

Blockade by methylation inhibitors of the anaphylactic response of guinea‐pig lung strips

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