Absence of a direct role for RNase HI in initiation of DNA replication at the oriC site on the Escherichia coli chromosome

Hong, Xiankang; Kogoma, Tokio

doi:10.1128/jb.175.20.6731-6734.1993

Cited by 19 publications

(10 citation statements)

References 23 publications

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“…pOC81 was a gift of W. Messer (Magee et al, 1992), and pTKQ27 (Kogoma, 1984) was previously described. pUC18::kan was constructed by inserting a kan gene at the XmnI site of pUC18 (Hong and Kogoma, 1993). ) was measured as described previously (Kogoma and Lark, 1975;Torrey and Kogoma, 1987).…”

Section: Media and Growth Conditionsmentioning

confidence: 99%

**Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase**

Hong

Cadwell

Kogoma

1996

Molecular Microbiology

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The conditions are described in which DNA replication can occur, in the absence of protein synthesis, in wild-type Escherichia coli cells. Chromosome replication, which is normally inhibited by addition of chloramphenicol, becomes resistant to this drug after nutritional shiftup, e.g. from minimal medium to Luria broth. This replication activity appears transiently when nutritionally upshifted cells enter stationary phase. The activity strictly requires recA+, but it is independent of recB+ and dnaA+. It can occur in the absence of concomitant transcription. Activation of the replication does not result from induction of the SOS response. As the characteristics of this DNA replication resemble those of the previously characterized stable DNA replication, it is termed nutritional shiftup-activatable stable DNA replication, nSDR. Possible mechanisms of the activation of nSDR in rapidly growing cells at the time of entry to stationary phase are discussed.

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Section: Media and Growth Conditionsmentioning

confidence: 99%

**Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase**

Hong

Cadwell

Kogoma

1996

Molecular Microbiology

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“…Another mode of SDR, cSDR, is constitutively observed in an rnhA mutant that lacks the major cellular RNase H activity (11). cSDR also depends on RecA protein, but not on RecB or RecC proteins (12). Whereas iSDR does not require transcription, cSDR completely depends on ongoing transcription.…”

mentioning

confidence: 99%

DNA Binding of PriA Protein Requires Cooperation of the N-terminal D-loop/Arrested-fork Binding and C-terminal Helicase Domains

Tanaka¹,

Mizukoshi²,

Taniyama³

et al. 2002

Journal of Biological Chemistry

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“…The bacterial strains used in this work are: WM2667 (AB1157 dnaA219 ) (Weigel et al , 1999), WM2840 (XL1‐Blue)/pBEX5BA‐ dnaA [1‐86]‐biotin (Weigel et al , 1999) and AQ8901 ( rnhA∷cam ) (Hong & Kogoma, 1993). SF53 ( dnaA219rnhA∷cam) was constructed by P1 transduction (Sambrook et al , 1989) of rnhA∷cam into strain WM2667.…”

Section: Methodsmentioning

confidence: 99%

A robust screen for novel antibiotics: specific knockout of the initiator of bacterial DNA replication

Fossum

Pascale

Weigel

et al. 2008

FEMS Microbiology Letters

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We have developed a novel type of a positive screen for the discovery of antibacterial compounds that target the Escherichia coli replication initiator protein DnaA. DnaA is an essential replication protein, conserved in (almost) all bacteria--including all human pathogens--and no existing antibiotics target the main components of the DNA replication machinery. This makes DnaA an attractive target and compounds discovered by this screen will constitute a new group of antibiotics. The conditional mutant, dnaA219, has a cold sensitive phenotype due to overreplication. In the screen, a DnaA inhibitor will reduce DnaA overactivity and thus restore growth at the nonpermissive temperature. This positive type of selection utilizes the rare phenomenon of lethal overactivity. In addition, the mutant strain has been made independent of DnaA activity by introduction of an alternative initiation pathway that allows growth under conditions of complete knockdown of DnaA. The resulting dnaA219rnhA strain is the basis of a robust, cell-based assay amenable to high-throughput screening. The screening assay has been validated against (1) a library of microbial fermentation extracts and (2) a known intracellular DnaA inhibitor.

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Absence of a direct role for RNase HI in initiation of DNA replication at the oriC site on the Escherichia coli chromosome

Cited by 19 publications

References 23 publications

**Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase**

**Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase**

DNA Binding of PriA Protein Requires Cooperation of the N-terminal D-loop/Arrested-fork Binding and C-terminal Helicase Domains

A robust screen for novel antibiotics: specific knockout of the initiator of bacterial DNA replication

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