Absence of a factor blocking a cellular cytotoxicity reaction in the serum of women with recurrent abortions

Fizet, D; Bousquet, Jean

doi:10.1111/j.1471-0528.1983.tb08943.x

Cited by 27 publications

(7 citation statements)

References 17 publications

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“…Therefore, P4 is different from normal im munosuppressive protein [ 17]; C-reactive pro tein appears only during the last trimester [18]; pregnancy proteins like PAPP-A [19] possess a subunit (200,000 daltons) but dis appear quickly after delivery and a specific immunserum doesn't absorb P4 activity. We have shown that P4 is not a placental prod uct; it appears in women taking oral contra ceptives [12], The important point is that P4 is absent from the serum of women with recurrent abortions [10]. It is suggested that P4 is nec essary for the maintenance of fetal allograft.…”

Section: Resultsmentioning

confidence: 84%

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Characterization of P4, a Natural Inhibitor of Cellular Cytotoxicity Reaction in Pregnant Women

Fizet

Bousquet

1985

Gynecol Obstet Invest

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Section: Resultsmentioning

confidence: 84%

“…We have brought to light the appearance during pregnancy of a factor able to inhibit nonspecifically a cellular cytotoxicity reac tion in vitro [10]. The sera of women with repeated spontaneous abortions were free from this protein [11].…”

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confidence: 99%

Characterization of P4, a Natural Inhibitor of Cellular Cytotoxicity Reaction in Pregnant Women

Fizet

Bousquet

1985

Gynecol Obstet Invest

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“…The technique used [10][11][12] makes it possible to measure the inhibitory' activity of different sera on a cytotoxicity reaction between in vitro sensibilized lymphocytes against allogenic lymphocytes from an HLA-incompatible donor. The degree of cytotoxicity was measured scoring the percentage of dead cells, using trypan blue dye exclusion.…”

Section: Cellular Cytotoxicity In Vitromentioning

confidence: 99%

“…[5] revealed the same immunosuppressive properties of glycopro teins in the serum of pregnant women, in those taking contraceptives and also in the sera of patients affected by malignant tu mors. A previous study [10], using the cellu lar cytotoxicity reaction in vitro, brought to light, in an original way, the appearance dur ing pregnancy of an immunosuppressive fac tor. This nonspecific protein seems to be dif ferent from those previously described by different authors.…”

Section: Introductionmentioning

confidence: 99%

Evidence of Immunosuppressor Factor in the Serum of Women Taking Oral Contraceptives

Bousquet¹,

Fizet²

1984

Gynecol Obstet Invest

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The serum of women taking oral contraceptives is able to inhibit an in vitro cytotoxicity reaction at the cell level. This effect is nonspecific. It seems to be due to the presence of an inhibitor of the lymphocyte activity and acting at the membrane level. This factor looks like that described in expectant mothers except that it exhibits a lower activity. This difference makes it possible to consider that its emergence depends on a specific hormonal impregnation.

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“…Other criteria include the lack of paternal HLA lymphocytotoxic antibodies [8][9][10] or the lack of mixed lymphocytic reaction (MLR) blocking antibodies [11][12][13]. These antibodies are commonly present in the sera of normal multiparous women but found to be lacking in the sera of recurrently aborting women [14][15][16][17][18][19][20].…”

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confidence: 99%

Paternal Leukocyte Immunization in Primary Recurrent Spontaneous Aborters

1993

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This was a pilot study to investigate the possible roles of human lymphocyte antigen (HLA), antipaternal lymphocytotoxic antibodies, and maternal antipaternal mixed lymphocyte reaction (MLR) blocking antibodies in the maintenance of pregnancy following paternal leukocyte immunization for patients with recurrent abortions. A total of 36 patients with unexplained, first trimester, primary recurrent spontaneous abortions were investigated for the detection of these two antibodies. There was a 43.3% rate of discordance in the presence of the two antibodies (P <0.05). A total of 26 of these women who lacked either antibody were immunized with paternal leukocytes on two occasions and the assays were repeated postimmunization. The seroconversion rate was 50% for lymphocytotoxic antibodies and 61.5% for maternal serum (MLR) blocking antibodies. Twenty patients achieved pregnancies postimmunization, 11 completed their pregnancies successfully, and 9 re-aborted. A total of 83.3% of patients who developed MLR blocking antibodies post-immunization had successful pregnancies while those who failed to seroconvert aborted again. This difference is statistically significant (P <.05). A total of 50% of patients who developed lymphocytotoxic antibodies post-immunization had successful pregnancies while only 40% who failed to seroconvert reaborted. This difference, we felt, was not statistically significant. The development of MLR blocking antibodies post-immunization is a better indicator of subsequent successful pregnancies than lymphocytotoxic antibodies.AM Bahar, AG White, IH Al-Abdullah, Paternal Leukocyte Immunization in Primary Recurrent Spontaneous Aborters. 1993; 13(2): 130-135 Certain instances of unexplained spontaneous abortions may be due to the failure of immunological recognition and adaptation to the fetoplacental unit. The precise nature of this interaction is not completely understood; however, one feature may be the failure of the fetus to stimulate in the mother protective antibodies that alter antigen expression as well as promote growth of the trophoblast [1,2].Treatment by immunization with paternal or donor leukocytes to stimulate antibody production to leukocyte trophoblast crossreactive epitopes has been tried by different groups with encouraging results [3]. In these groups, the criteria for selection of patients for immunotherapy differ. One criterion is the sharing of HLA antigens [4]. There is evidence of increased antigen sharing between recurrently aborting women and their partners with sharing frequencies more than can be expected by chance alone [5][6][7]. Other criteria include the lack of paternal HLA lymphocytotoxic antibodies [8][9][10] or the lack of mixed lymphocytic reaction (MLR) blocking antibodies [11][12][13]. These antibodies are commonly present in the sera of normal multiparous women but found to be lacking in the sera of recurrently aborting women [14][15][16][17][18][19][20].The results of immunotherapy on successful pregnancy outcome using different criteria for selection are...

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Absence of a factor blocking a cellular cytotoxicity reaction in the serum of women with recurrent abortions

Cited by 27 publications

References 17 publications

Characterization of P4, a Natural Inhibitor of Cellular Cytotoxicity Reaction in Pregnant Women

Characterization of P4, a Natural Inhibitor of Cellular Cytotoxicity Reaction in Pregnant Women

Evidence of Immunosuppressor Factor in the Serum of Women Taking Oral Contraceptives

Paternal Leukocyte Immunization in Primary Recurrent Spontaneous Aborters

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