Absence of Dominance of the Cu Gene in Influencing o‐Hydroxycinnamic Acid Content in
            <i>Melilotus alba</i>
            <sup>1</sup>

Gorz, H. J.; Haskins, Francis A.

doi:10.2135/cropsci1969.0011183x000900010027x

Cited by 8 publications

(9 citation statements)

References 8 publications

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“…If sweetclover ,B-glucosidase has such a role, a pronounced effect of the b gene on plant height, for example, would be expected. The absence of significant height differences between BB and bb plants (5,6) suggests that in sweetclover, this enzyme may not have a large influence on wall extensibility.…”

Section: Resutlts and Discussionmentioning

confidence: 99%

“…of the CuCuBB genotype. The derivation of this genotype has been described elsewhere (5). Leaves ,B-Glucosidase was extracted from the hypocotyls of beans (Phaseolus vulgaris L. var.…”

Section: Methodsmentioning

confidence: 99%

“…Preincubation mixtures consisted of 1.0 ml of deionized enzyme preparation; 0.5 ml of water, 81 mm acetate buffer, pH 5.0, or 81 mm borate buffer, pH 8.5; and, in some instances, 5 mg of cellular debris. Mixtures were allowed to stand for 5 min at room temperature and were then centrifuged.…”

Section: Methodsmentioning

confidence: 99%

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Solubility of β-Glucosidase in Homogenates of Sweetclover Leaves and Bean Hypocotyls

et al. 1972

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In a recent paper Eberhart and Beck (4) suggested that the aryl-,B-glucosidase of Neurospora conidia is a mural enzyme. Similarly, Nevins (11) reported that ,-glucosidase is strongly associated with cell walls during extraction of bean hypocotyls at pH 4.6 and suggested that this enzyme is wall-bound in situ, and Keegstra and Albersheim (9) ,B-Glucosidase was extracted from the hypocotyls of beans (Phaseolus vulgaris L. var. Red Kidney) which had been allowed to germinate for 5 days at room temperature between moist paper towels. The bean seeds were obtained from a local market. Extraction and centrifugation procedures were similar to those used for sweetclover preparations.The cellular debris used in some preincubation experiments was prepared from sweetclover leaves which had been frozen in liquid nitrogen, pulverized in a mortar, lyophilized, and stored in a freezer. A 1-g portion of this material was suspended in 20 ml of 50 mm borate buffer, pH 8.5, and the suspension was centrifuged at 17,000g for 10 min. The pellet was subjected to three additional washings with buffer, one with water, and a final washing with acetone. The washed pellet was air-dried.Preincubation mixtures consisted of 1.0 ml of deionized enzyme preparation; 0.5 ml of water, 81 mm acetate buffer, pH 5.0, or 81 mm borate buffer, pH 8.5; and, in some instances, 5 mg of cellular debris. Mixtures were allowed to stand for 5 min at room temperature and were then centrifuged. Supernatant fractions from this centrifugation were assayed for ,Bglucosidase activity. Pellets were resuspended in 1.5 ml of 50 mM borate buffer, pH 8.5, and recentrifuged. Assay of BO-glucosidase activity in the supernatant fractions from this centrifugation provided a measure of the activity which was associated with the pellet at the end of the preincubation treatment.

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Section: Resutlts and Discussionmentioning

confidence: 99%

“…of the CuCuBB genotype. The derivation of this genotype has been described elsewhere (5). Leaves ,B-Glucosidase was extracted from the hypocotyls of beans (Phaseolus vulgaris L. var.…”

Section: Methodsmentioning

confidence: 99%

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Solubility of β-Glucosidase in Homogenates of Sweetclover Leaves and Bean Hypocotyls

et al. 1972

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“…Pl ants or the C u.Cii genotype are hi gh in content of o-H CA glu cosid es, a nd preparations of th e BB ge n oty pe pos sess f3-glucosidase activity (6,10). The c « and B genes are both lacking in d ominan ce (3,7). These alleles, derived from M. dcn iata (Wa lds t, & Kit.)…”

Section: (Mcl Il Otlls Albamentioning

confidence: 99%

Cross‐Fertilization in Melilotus Alba¹

Gorz¹,

Haskins²

1971

Crop Science

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Two simply inherited characters, o‐hydroxycinnamic acid (o‐HCA) content and β‐glucosidase activity, were used as genetic markers in studying the frequency of cross‐fertilization in Melilotus alba Desr. Based on the o‐HCA marker, the effective cross‐fertilization averaged 58% for two harvest years in a biennial population and 67% in a 1‐year study with an annual population. Use of the β‐glucosidase marker provided a cross‐pollination estimate of 72% in the biennial population. The extent of natural crossing in sweetclover appears to be sufficient to restore normal vigor in the production of varieties synthesized from selected inbred lines.

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“…Plants of the CuCu genotype are high in content of o-hydroxycinnamic acid glucosides, and preparations of the BB plants possess ,a-glucosidase activity. The Cu and B genes are both lacking in dominance (6,10).…”

Section: Genetic Change and Variety Contaminationmentioning

confidence: 99%

Evaluation of Cross‐fertilization in Forage Crops¹

Gorz¹,

Haskins²

1971

Crop Science

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SummaryIn the improvement of forage crop varieties knowledge of the extent of cross‐fertilization is of value for (1) determining the extent and randomness of crossing in breeding procedures, (2) estimating varietal changes due to contamination and mechanical mixtures, and (3) evaluating effects of cross‐fertilization in the development and production of synthetic and hybrid combinations. Genetic markers can aid greatly in the evaluation of cross‐fertilization, and thus in the development and production of new varieties. Ideally, markers should be distinctive and readily detected in the seedling stage, conditioned by a single dominant gene with complete penetrance, and have no adverse effects on plant growth, zygote viability, or randomness of cross‐pollination.

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Absence of Dominance of the Cu Gene in Influencing o‐Hydroxycinnamic Acid Content in Melilotus alba ¹

Cited by 8 publications

References 8 publications

Solubility of β-Glucosidase in Homogenates of Sweetclover Leaves and Bean Hypocotyls

Solubility of β-Glucosidase in Homogenates of Sweetclover Leaves and Bean Hypocotyls

Cross‐Fertilization in Melilotus Alba¹

Evaluation of Cross‐fertilization in Forage Crops¹

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Absence of Dominance of the Cu Gene in Influencing o‐Hydroxycinnamic Acid Content in Melilotus alba 1

Cited by 8 publications

References 8 publications

Solubility of β-Glucosidase in Homogenates of Sweetclover Leaves and Bean Hypocotyls

Solubility of β-Glucosidase in Homogenates of Sweetclover Leaves and Bean Hypocotyls

Cross‐Fertilization in Melilotus Alba1

Evaluation of Cross‐fertilization in Forage Crops1

Contact Info

Product

Resources

About

Absence of Dominance of the Cu Gene in Influencing o‐Hydroxycinnamic Acid Content in Melilotus alba ¹

Cross‐Fertilization in Melilotus Alba¹

Evaluation of Cross‐fertilization in Forage Crops¹