Absence of Epstein-Barr virus EBER-1 transcripts in an epidemiologically diverse group of breast cancers

Glaser, Sally L.; Ambinder, Richard F.; DiGiuseppe, Joseph A.; Horn‐Ross, Pamela L.; Hsu, Joe L.

doi:10.1002/(sici)1097-0215(19980209)75:4<555::aid-ijc10>3.0.co;2-8

Cited by 79 publications

(54 citation statements)

References 26 publications

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“…In our study radioactive ISH signal intensities were low in paraffin-embedded sections. The absence of labelling that has been reported by others (Chang et al, 1992;Lespagnard et al, 1995;Glaser et al 1998) could be a result of technical problems, such as the fixation as reported by Penault-Llorca et al (1994), or of the low sensitivity of non-radioactive ISH.…”

Section: Discussionmentioning

confidence: 82%

Frequency and genome load of Epstein–Barr virus in 509 breast cancers from different geographical areas*

et al. 2001

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Since the few data exploring a possible association between Epstein–Barr virus (EBV) and breast cancer are conflicting, we investigated this association together with the influences of geographical areas. 509 breast cancers were sampled from areas with varying risks of nasopharynx carcinoma (NPC) such as North Africa (Algeria and Tunisia, high-risk area); southern France (Marseille, intermediate-risk area); and northern Europe (northern France, the Netherlands and Denmark; low-risk areas). Polymerase chain reaction (PCR) of a subregion of EBV BamHIC encoding the EBERs demonstrated that 31.8% of the tumours contained the viral genome. No significant differences were observed among the geographical areas. However, positive samples showed higher loads of the EBV genome in the NPC high- and intermediate-risk areas than in the low-risk areas. EBV type 1 was the dominant strain. In situ hybridization studies using a 35 S-labelled riboprobe for EBER1 and a laser capture microdissection, combined with quantitative PCR, showed that EBV localization was restricted to some tumour epithelial cell clusters. EBV could not be detected in the stroma. Considering the whole population covered, the presence of the EBV genome was not correlated with age, menopausal status, tumour, size, nodal status or histological grade. © 2001 Cancer Research Campaign http://www. bjcancer.com

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Section: Discussionmentioning

confidence: 82%

Frequency and genome load of Epstein–Barr virus in 509 breast cancers from different geographical areas*

et al. 2001

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“…One study reported multiple and bilateral fibroadenomas in immunosuppressed patients receiving cyclosporin A (17). Isolated studies have investigated the EBV/breast cancer association with contradictory results (2,18,19). This is the first study to investigate the role of EBV in rapidly growing breast tumors in immunocompromised patients.…”

Section: Discussionmentioning

confidence: 99%

“…In the Bonnet (2) and Labreque (18) studies, in which they demonstrated the presence of EBV genome using PCR, only a handful of samples were analyzed by another EBV detection technique such as IHC. In the negative studies of Chu et al (22,23) and Glasser et al (19), EBER-2 PCR amplification was not performed. Our results show that different EBV detection techniques often display seemingly contradictory results, possibly because their sensitivity depends on sample-specific preparation at the time of fixation, or subsequent alterations of the specimens over time.…”

Section: Discussionmentioning

confidence: 99%

Detection of Epstein-Barr Virus in Rapidly Growing Fibroadenomas of the Breast in Immunosuppressed Hosts

et al. 2002

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Fibroadenomas are the most common benign tumors of the female breast and are associated with a slight increase in the risk of subsequent breast cancer. Multiple fibroadenomas have been described in patients after renal transplantation and are thought to be secondary to drug-related growth stimulation. Epstein-Barr virus (EBV) has been detected in many neoplasms, including breast cancer. We set out to investigate whether EBV plays a role in the development of rapidly growing fibroadenomas in immunocompromised patients. We studied 19 fibroadenomas and one invasive ductal carcinoma that developed after organ transplantation or treatment for lupus erythematosus. As a control group we included 11 fibroadenomas from non-immunocompromised patients. DNA was amplified using polymerase chain reaction (PCR) of the EBV-encoded small RNA (EBER-2) DNA sequence. EBV latent membrane protein 1 (LMP-1) transcripts were amplified using reverse transcription (RT) PCR. Immunohistochemical (IHC) staining for LMP-1 protein was performed. A total of 9 out of 20 tumors (45%) were concordantly positive by PCR and IHC. IHC stained exclusively the epithelial cells. All the fibroadenomas in non-immunocompromised patients were negative for LMP-1 (Fisher's exact test P ‫؍‬ .0006). These data suggest that EBV is associated with fibroadenomas in this immunosuppressed population and that the infection is specifically localized to epithelial cells. This is the first study suggesting a role for EBV in the pathogenesis of fibroadenomas.

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“…In fact, where GAPDH is low, EBV genome copy number is high in one of their two samples studied. Glaser et al (1998) reported negative results in their study of 107 cases using EBER1 transcripts by in situ hybridization, which included one with subsequent NPC and one in which lymphocytes were EBERpositive. This study covered a diverse population from 21 hospitals in 7 counties from the San Francisco Bay Area of northern California to reflect the spectrum of age, sex, and ethnic groups associated with variation in breast cancer incidence in the United States.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have suggested an etiological role for EBV in breast cancer. These studies remain controversial because other studies have questioned this association (Bonnet et al, 1999;Brink et al, 2000;Chu et al, 1998Chu et al, , 2001Fina et al, 2001;Gaffey et al, 1993;Glaser et al, 1998;Horiuchi et al, 1994;Labrecque et al, 1995;Lespagnard et al, 1995;Magrath and Bhatia, 1999;McCall et al, 2001;Touitou et al, 2001;Yasui et al, 2001). These reports have mainly used the techniques of Southern blotting and PCR for the detection of the EBV genome.…”

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confidence: 99%

Lack of Expression of the Epstein-Barr Virus (EBV) Gene Products, EBERs, EBNA1, LMP1, and LMP2A, in Breast Cancer Cells

Deshpande

Badve

Kidwai

et al. 2002

Laboratory Investigation

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SUMMARY:Epstein-Barr virus (EBV), a gamma herpesvirus, has been associated with a variety of human malignancies such as Burkitt's lymphoma, Hodgkin's lymphoma, NPC, and gastric cancer. A controversy regarding the association of EBV with breast cancers has recently been reported in the literature. These reports have mainly used the DNA detection techniques of polymerase chain reaction and Southern blot hybridization, with the inherent lacunae associated with these techniques for signal localization. Our group has studied EBV association with breast cancer by using in situ hybridization for detecting nonpolyadenylated EBV RNA (EBERs), along with using protein localization technique of immunohistochemistry, studying the EBV nuclear antigen 1 (EBNA1) and the latent membrane proteins (LMP1 and LMP2A). This is the first article analyzing the expression of LMP2A in breast cancer cells. In all of our 43 female breast cancer cases under study, we failed to detect expression of any of the EBV viral gene products tested. (Lab Invest 2002, 82:1193-1199.

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Absence of Epstein-Barr virus EBER-1 transcripts in an epidemiologically diverse group of breast cancers

Cited by 79 publications

References 26 publications

Frequency and genome load of Epstein–Barr virus in 509 breast cancers from different geographical areas*

Frequency and genome load of Epstein–Barr virus in 509 breast cancers from different geographical areas*

Detection of Epstein-Barr Virus in Rapidly Growing Fibroadenomas of the Breast in Immunosuppressed Hosts

Lack of Expression of the Epstein-Barr Virus (EBV) Gene Products, EBERs, EBNA1, LMP1, and LMP2A, in Breast Cancer Cells

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