Absence of Glycoproteins in Poliovirus Particles

Abstract

SUMMARYProperly purified preparations of poliovirus particles are practically free of o-galactose and N-acetyl-D-glucosamine. These sugars, however, are found in crude virus preparations, obtained by high and low speed sedimentation and by CsCl-gradient fractionation from infected HeLa cells. They are separated from the virus particle by extraction with chloroform and subsequent isopycnic sedimentation of the virus preparation. Since o-galactose and N-acetyl-o-glucosamine are regular constituents of glycoproteins, the absence of significant amounts of these sugars demonstrates the lack of glycoproteins in poliovirus particles.Radioactive monosaccharides are recommended for the detection of cellular impurities in carbohydrate-free viruses.A simple and rapid method for the purification of large quantities of poliovirus by 'precipitation' with polyethylene glycol, resuspension in CsC1 solutions, extraction with chloroform and isopycnic sedimentation is described.

Entry of Poliovirus Type 1 and Mouse Elberfeld (ME) Virus into HEp-2 Cells: Receptor-mediated Endocytosis and Endosomal or Lysosomal Uncoating

“…The mechanism for the uncoating and delivery of the poliovirus and ME virus RNA from the endosomes and/or lysosomes into the cytoplasm remains unclear. A fusion of virus components with the membranes of these acidic organelles as shown for SFV (Helenius et al, 1983) seems improbable, as picornaviruses (unlike SFV) have no envelope or glycosylated proteins (Drzeniek & Bilello, 1974). Analysis of the contribution of lysosomal and endosomal enzymes to virus capsid degradation under different pH conditions should give insight into the uncoating process of picornaviruses.…”

In vitro Stimulation of Presensitized Mouse Spleen Cells with Poliovirus Type 1, Mahoney, and Enhancement of Poliovirus-specific Hybridomas

“…Poliovirus type 1, Mahoney, and naturally occurring empty capsids (NECs) were prepared according to standard procedures (Drzeniek & Bilello, 1974). Pure poliovirus polypeptides were obtained by reversed phase HPLC in 609/00 formic acid and a gradient of acetonitrile (Heukeshoven & Dernick, 1985).…”

The Effect of Cesium Salts on Dense Poliovirus Particles

“…Poliovirus type 1, strain Mahoney, was used. Production and purifi cation of DP particles and standard poliovirus have been described previously [11,14,20]. 10 x PBS is a concentrate of phosphate-buffered saline, pH 7.2, containing 0.2 m K,Naphosphate and 1.2 m NaCl.…”