Absence of leucine in an essential amino acid supplement reduces activation of mTORC1 signalling following resistance exercise in young females

Abstract

Absence of leucine in an essential amino acid supplement reduces activation of mTORC1 signalling following resistance exercise in young females.. were not significantly altered after 3h recovery from exercise. In conclusion, the presence of leucine in the supplement enhances the stimulatory effect on mTORC1 signaling and reduces the level of tyrosine and the sum of the EAA in muscle and plasma, suggesting a stimulation of protein synthesis and/or inhibition of breakdown, leading to improvement in net protein balance.

Native whey induces higher and faster leucinemia than other whey protein supplements and milk: a randomized controlled trial

“…Nonetheless, leucinemia has yet to be regarded as an exclusive predictor of MPS. Studies have shown leucine to be an important stimulus for the intracellular kinases regulating MPS, such as mTOR and p70 S6K [26,27]. Based on these studies the high content of leucine and BCAA, and its rapid aminoacidemia suggest a great potential for native whey to increase acute post-exercise intracellular anabolic signalling and MPS.…”

Regulation of metabolic health and aging by nutrient-sensitive signaling pathways

“…In contrast to GCN2, which is more sensitive to the essential amino acids, mTOR activity is especially sensitive to a few key amino acids. mTORC1 is particularly sensitive to the BCAAs, which potently activate mTORC1 in vitro in cell culture, and in vivo in both rodents and humans (Blomstrand et al, 2006; Gallinetti et al, 2013; Li et al, 2011; Moberg et al, 2014; Sans et al, 2006; Xiao et al, 2011). It is therefore logical to consider if restriction of dietary protein may promote longevity in part through inhibition of mTOR signaling, particularly in light of recent studies demonstrating that mTORC1 inhibition is required for the beneficial effects of protein restriction on stress resistance after hepatic injury (Harputlugil et al, 2014).…”

“…As summarized by Christopher Lynch over a decade ago, experiments in a variety of cell types have demonstrated that “most of the effects of amino acids on mTOR signaling are abolished by lowering the concentration of leucine, or mimicked by adding leucine and, to a lesser extent, the other branched-chained amino acids” (Lynch, 2001). Indeed, the three BCAAs potently activate mTORC1 activity in not only in cell culture, but also in vivo in rodents and humans (Blomstrand et al, 2006; Gallinetti et al, 2013; Li et al, 2011; Moberg et al, 2014; Sans et al, 2006; Xiao et al, 2011). …”

Efectos de los suplementos de proteína y aminoácidos de cadena ramificada en entrenamiento de fuerza: revisión bibliográfica

“…Son muchas las investigaciones que han estudiado los posibles efectos asociados a los suplementos de proteína y/o BCAAs en torno a la ganancia de masa muscular [16][17][18][19] , fuerza 20,21 , composición corporal de los sujetos [20][21][22] , atenuación de la fatiga 23,24 , reducción del dolor y daño muscular [25][26][27] y el efecto de la respuesta anabólica mediante la activación de una serie de enzimas que permiten el aumento de la síntesis de proteí-nas [28][29][30][31][32] .…”

“…La fosforilación de la p70S6K (perteneciente a la proteína ribosomal S6) aumenta en todos los grupos experimentales después del ejercicio y la ingesta de proteínas y/o BCAAs 30,32,[61][62][63] . Burke y Cols.…”