Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA

Saragosti, Sentob; Moyne, Gilles; Yaniv, Moshe

doi:10.1016/0092-8674(80)90235-4

Cited by 371 publications

(192 citation statements)

References 21 publications

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“…(16,17). Secondly, electron micrographs of SV40 chromatin (18,19) show the same kind of zigzag structure which has been found in systematic electron microscope studies on cellular chromatin, and which has been attributed to the presence of HI on the side of the nucleosome at the entry and exit points of the DNA (20 (20)). …”

Section: Is There a Linkage Number Problem?mentioning

confidence: 73%

The helical periodicity of DNA on the nucleosome

Klug¹,

Lutter²

1981

Nucl Acids Res

165

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Section: Is There a Linkage Number Problem?mentioning

confidence: 73%

The helical periodicity of DNA on the nucleosome

Klug¹,

Lutter²

1981

Nucl Acids Res

165

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“…Thus Simian Virus 40 DNA, which in infected cells forms nucleosomes (7), was found to possess a nuclease-sensitive region localized around the origin of replication and containing a promotor region of about 300-400 pairs in length (7). This region appears to be devoid of nucleosomes as shown by electron microscopy (8)(9)(10). In vitro experiments have shown that using purified histones H2A, H2B, H3 and H4, the reconstitution of nucleosomes is possible with polydeoxynucleotides composed of alternating purine- (11,12,13).…”

Section: Introductionmentioning

confidence: 99%

Conformation of DNA in chromatin reconstituted from poly [d(A-T)] and the core histones

Brahms¹,

Brahmachari²,

Angelier³

et al. 1981

Nucl Acids Res

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Present results provide direct evidence of the nature of a conformational change in DNA when nucleosomes are formed from core histones and poly fd(A-T)]. First, we have found some features which have characteristic aspects of the A like conformation of DNA. Thus, an increased contribution due to a sugar conformation close to C3'-endo puckering is detected in the Raman spectra. In addition, the circular dichroism (C.D.) spectra of reconstituted chromatin with poly PI(A-T)j exhibits an increased intensity at about 262 nm.A second feature acquired by poly rd(A-T)l in nucleosome formation from core histones is related to the presence of a negative band at about 280 nm in the C.D. spectra. The nature of this change is correlated with a DNA conformation characterized by a decreased number of base pairs per turn (28,29). This indicates that these two features of reconstituted nucleosomes reflect the presence of two types of DNA conformations, which overall form is of the B type (22,36).

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“…These hypersensitive sites have been observed adjacent to genes encoding heat-shock proteins (2, 3), 3-globin (4-6), histones (7), a Drosophila glue protein (8) and larval cuticle gene cluster (9), and on SV40 minichromosomes (10). Furthermore, these sites have been associated with many different sequences which are involved in gene expression and frequently their appearance has been correlated with developmental and tissue-specific gene activation (4-6, 8, the gene is expressed (13,17).…”

Section: Introductionmentioning

confidence: 99%

“…Enhancer elements also exhibit DNase I hypersensitivity at or near them. The 72 bp repeat enhancer of the SV40 minichromosome is hypersensitive to DNase I (10,14). Even translocation of the enhancer 3.5 kb upstream preserved its function and DNase I hypersensitivity (23).…”

Section: Introductionmentioning

confidence: 99%