Absence of TetB identifies minocycline-susceptible isolates of Acinetobacter baumannii

Lomovskaya, Olga; Sun, Dongxu; Rubio-Aparicio, Debora; Nelson, Kirk; Thamlikitkul, Visanu; Dudley, Michael N.; Redell, Mark

doi:10.1016/j.ijantimicag.2018.04.006

Cited by 16 publications

(19 citation statements)

References 11 publications

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“…Previous studies confirmed that tetB confers resistance to minocycline and doxycycline. Lomovskaya et al indicated that the absence of tetB can infer susceptibility to minocycline, but, we found tetB in susceptible strains [29,31]. Interestingly, tetB was identified in all minocycline resistance strains and was associated with high rate of resistance to minocycline.…”

Section: Discussioncontrasting

confidence: 55%

Detection of efflux pump genes in multiresistant Acinetobacter baumannii ST2 in Iran

Meshkat

Salimizand

Amini

et al. 2021

AMicr

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Acinetobacter baumannii, as a nosocomial pathogen has become a worldwide concern in recent years. In the current study, the resistance to tetracyclines and colistin were assessed in the isolates from different provinces of Iran.During the timeline of this study, a number of 270 isolates of A. baumannii were collected from tracheal aspirates, wounds, urine and blood cultures. The minimum inhibitory concentration (MIC) for tetracycline, doxycycline, minocycline, tigecycline and colistin were evaluated. Tetracycline resistance genes were assessed by PCR. The mean expression level of adeB, adeJ and adeG were assessed using semi quantitative Real-Time PCR. The clonal relationship of the isolates was evaluated by the repetitive extragenic palindromic PCR (REP-PCR), International Clonal (IC) Lineage Multiplex PCR and multilocus sequence typing (MLST) (Pasteur scheme) methods.The MIC by microdilution method showed that 87.5, 51.4, 28, 0.74 and 0% of the isolates were resistant to tetracycline, doxycycline, minocycline, tigecycline and colistin respectively. The prevalence of tetracycline resistance genes was 99.2, 99.2, 98, 86.7, 10, 3.33, 0.37, 0% for adeB, adeJ, adeG, tetB, tetA(39), tetA, tetM and tetH in tetracycline-resistant isolates. Moreover, the expression level of adeB, adeJ, adeG genes in tigecycline-nonsusceptible A. baumannii (TNAB) strain was higher compared to the tigecycline-susceptible A. baumannii (TSAB). A broad genomic diversity was revealed, but ST2 was the most prevalent ST. Our results indicated that tetracycline resistance in Iran is mediated by resistance-nodulation-cell division (RND) and tetB efflux pumps.

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Section: Discussioncontrasting

confidence: 55%

Detection of efflux pump genes in multiresistant Acinetobacter baumannii ST2 in Iran

Meshkat

Salimizand

Amini

et al. 2021

AMicr

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“…The novel aminomethylcycline omadacycline is a welcomed addition to the available tetracycline derivatives given its potency against TetB positive, minocycline-resistant CNSAb (~70% of A. baumannii ), once daily oral dosing, and favorable PK properties including low protein binding and enhanced intrapulmonary penetration ( 14 , 27 , 28 ). Tetracycline derivatives, including omadacycline, have demonstrated little antibacterial activity against A. baumannii alone in in vitro pharmacodynamic models, as expected given their low free serum concentrations and bacteriostatic nature ( 29 – 31 ).…”

Section: Discussionmentioning

confidence: 99%

Activity of Omadacycline Alone and in Combination against Carbapenem-Nonsusceptible Acinetobacter baumannii with Varying Minocycline Susceptibility

et al. 2022

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Treatment of infections due to Acinetobacter baumannii often involves the use of multiple antibiotics simultaneously as combination therapy, but it is unknown which antibiotics are best used together. Tetracycline agents such as minocycline and tigecycline maintain good activity against A. baumannii and are often used with one or more other agents to achieve better killing of the bacteria.

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“…For example, Tet(A) efflux pumps in MDR A. baumannii export tetracycline and doxycycline, but not minocycline. , However, the susceptibility of these strains to minocycline deteriorates in the presence of the Tet(B) efflux pump . Despite the continued importance of minocycline and doxycycline in the clinic, their efficacy is still compromised by RPPs, tetracycline inactivating enzymes, and most efflux pumps, leading to the development of a new class of semisynthetic tetracyclines, called glycylcyclines.…”

Section: Tetracyclinesmentioning

confidence: 99%

“…166,167 However, the susceptibility of these strains to minocycline deteriorates in the presence of the Tet(B) efflux pump. 168 Despite the continued importance of minocycline and doxycycline in the clinic, their efficacy is still compromised by RPPs, tetracycline inactivating enzymes, and most efflux pumps, leading to the development of a new class of semisynthetic tetracyclines, called glycylcyclines. The glycylcyclines are semisynthetic tetracycline derivatives that harbor a glycylamido group at the C-9 position.…”

Section: Semi-synthetic Tetracyclinesmentioning

confidence: 99%

The Antibiotic Resistome: A Guide for the Discovery of Natural Products as Antimicrobial Agents

2021

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The use of life-saving antibiotics has long been plagued by the ability of pathogenic bacteria to acquire and develop an array of antibiotic resistance mechanisms. The sum of these resistance mechanisms, the antibiotic resistome, is a formidable threat to antibiotic discovery, development, and use. The study and understanding of the molecular mechanisms in the resistome provide the basis for traditional approaches to combat resistance, including semisynthetic modification of naturally occurring antibiotic scaffolds, the development of adjuvant therapies that overcome resistance mechanisms, and the total synthesis of new antibiotics and their analogues. Using two major classes of antibiotics, the aminoglycosides and tetracyclines as case studies, we review the success and limitations of these strategies when used to combat the many forms of resistance that have emerged toward natural product-based antibiotics specifically. Furthermore, we discuss the use of the resistome as a guide for the genomicsdriven discovery of novel antimicrobials, which are essential to combat the growing number of emerging pathogens that are resistant to even the newest approved therapies.

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Absence of TetB identifies minocycline-susceptible isolates of Acinetobacter baumannii

Cited by 16 publications

References 11 publications

Detection of efflux pump genes in multiresistant Acinetobacter baumannii ST2 in Iran

Detection of efflux pump genes in multiresistant Acinetobacter baumannii ST2 in Iran

Activity of Omadacycline Alone and in Combination against Carbapenem-Nonsusceptible Acinetobacter baumannii with Varying Minocycline Susceptibility

The Antibiotic Resistome: A Guide for the Discovery of Natural Products as Antimicrobial Agents

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