Absence of the nahG-like gene caused the syntrophic interaction between Marinobacter and other microbes in PAH-degrading process

Wang, Chongyang; Huang, Yong; Zhang, Zuotao; Hao, Han; Wang, Hui

doi:10.1016/j.jhazmat.2019.121387

Cited by 46 publications

(23 citation statements)

References 45 publications

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“…3 PHE, close to 70%). Similar results from recent study have been reported that strain N4 could only nearly 80% of the PHE had been degraded in 8 days 13 . The concentration of intermediate product E (Fig.…”

Section: Dynamic Changes Of Metabolic Intermediates and Phesupporting

confidence: 91%

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Fast Location and Isolation of Phenanthrene-degrading Halophilic Microbial Community and its Degradation Pathway

Peng¹,

Yuan²,

Li³

et al. 2021

Preprint

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Microbial consortium WZ-4, which could degrade phenanthrene (PHE) as the main carbon and energy source, was isolated from the aerobic sludge of Weizhou wastewater treatment plant. Under the condition of high salinity (3%), the degradation of PHE (100 mg/L) was 87.76% in 7d. Its metabolites, genome sequence and biodegradation pathway were studied. The main metabolites include 1,2-dihydroxynaphthalene, 1-hydroxy-2-naphthalene, 5,6-benzocoumarin and phthalic acid. 12 PHE degrading enzyme genes appeared in the metagenome sequencing of WZ-4, and the genes involved in PHE degradation were included phdE, phdF, phdG, and pcaL. Based on the metabolites detected by GC-MS and the potential PHE-degrading genes identified by BLAST search, biodegradation pathway of PHE by WZ-4 was predicted.

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Section: Dynamic Changes Of Metabolic Intermediates and Phesupporting

confidence: 91%

“…In the process of aerobic PHE degradation, the cracking of a benzene ring usually starts from the hydroxyl containing benzene ring 13 . According to previous studies, the biodegradation of PHE usually through the double hydroxylation of the C1-C2 pathway or C3-C4 pathway 20,21 .…”

Section: Degradation Pathway Of Phe By Microbial Consortium Wz-4mentioning

confidence: 99%

Fast Location and Isolation of Phenanthrene-degrading Halophilic Microbial Community and its Degradation Pathway

Peng¹,

Yuan²,

Li³

et al. 2021

Preprint

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“…Samples of 3 mL cell suspension were collected once or twice per day after incubation and extracted using 5 mL dichloromethane for 30 minutes. After ltration, the residual phenanthrene was further measured by HPLC (Shimudzu, Japan) as previously described (Wang et al, 2019).…”

Section: The Detection Of Residual Phenanthrene Concentration and Metabolic Intermediatesmentioning

confidence: 99%

“…Derivatization experiment was further processed using Bis (trimethylsilyl) tri uoroacetamide (BSTFA and TMCS, volume ratio: 99/1) to replace the active hydrogen atom in phenanthrene metabolic intermediates by TMS group (Si(CH 3 ) 3 ) with m/z 73. Then, the derivatized samples and standard compounds (1-hydroxy-2-naphthoic acid, 1,2-dihydroxynaphthalene, catechol, and gentisic acid) were detected using GC-MS (Agilent 7890A GC, Inert MSD with TripleAxis Detector, Agilent, USA) according to previously report (Wang et al, 2019).…”

Section: The Detection Of Residual Phenanthrene Concentration and Metabolic Intermediatesmentioning

confidence: 99%

Degradation of Polycyclic Aromatic Hydrocarbons by Consortium 5H Under Hypersaline Conditions

Jin

Zhang

Han

et al. 2021

Preprint

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PAHs were widely detected accumulated in saline and hypersaline environments. The halotolerant and halophilic microbes were considered as the most suitable player for the elimination of PAHs in such environments. In this study, consortium 5H was enriched under 5% salinity that was able to completely degrade phenanthrene in 5 days. By high-throughput sequencing, consortium 5H was identified mainly composed of Methylophaga, Marinobacter and Thalassospira. Combined with the investigation of intermediates and enzymatic activities, the degradation pathway of consortium 5H on phenanthrene was proposed. Meanwhile, consortium 5H was identified with ability to tolerate a wide range of salinity (1% to 10%) and initial PAHs concentration (50 mg/L to 400 mg/L). It was also able to work under neutral to week alkaline conditions (pH from 6 to 9) and the phytotoxicity of the produced intermediates showed no significant difference with distilled water. This study expanded the knowledge of PAH-degradation under hypersaline environments and consortium 5H was proposed with a good potential for the elimination of PAHs pollution under saline/hypersaline environments.

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“…Typical examples include syringate degradation via sequential crossfeeding between Acetobacterium woodii and Pelobacter acidigallici 10 , phenanthrene degradation between Marinobacter sp. N4 and other PAH-degrading microbes in marine environments 11 , as well as atrazine degradation through MDOL within four bacterial species 12 . Nevertheless, all of these natural communities grow in the environments that may fluctuate over time and vary in space.…”

Section: Introductionmentioning

confidence: 99%

Substrate traits govern the assembly and spatial organization of microbial community engaged in metabolic division of labor

Fang

Chen

Nie

et al. 2020

Preprint

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Metabolic division of labor (MDOL) is widespread in nature, whereby a complex metabolic pathway is shared between different strains within a community for mutual benefit. However, little is known about how communities engaged in MDOL assemble and spatially organize. We hypothesized that when degradation of an organic compound is carried out via MDOL, substrate concentration and its toxicity modulate the benefit allocation between the two microbial populations, thus governing the assembly of this community. We tested this hypothesis by combining individual-based simulations with pattern formation assays using a synthetic microbial community. We found that while the frequency of the first population increases with an increase in substrate concentration, this increase is capped with an upper bound determined by the biotoxicity of the substrate. In addition, our model showed that substrate concentration and its toxicity affect levels of intermixing between strains. These predictions were quantitatively verified using an engineered system composed of two strains degrading salicylate through MDOL. Our results demonstrate that the structure of the microbial communities can be quantitatively predicted from simple environmental factors, such as substrate concentration and its toxicity, which provides novel perspectives on understanding the assembly of natural communities, as well as insights into how to manage artificial microbial systems.

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Absence of the nahG-like gene caused the syntrophic interaction between Marinobacter and other microbes in PAH-degrading process

Cited by 46 publications

References 45 publications

Fast Location and Isolation of Phenanthrene-degrading Halophilic Microbial Community and its Degradation Pathway

Fast Location and Isolation of Phenanthrene-degrading Halophilic Microbial Community and its Degradation Pathway

Degradation of Polycyclic Aromatic Hydrocarbons by Consortium 5H Under Hypersaline Conditions

Substrate traits govern the assembly and spatial organization of microbial community engaged in metabolic division of labor

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