2012
DOI: 10.1063/1.3699273
|View full text |Cite
|
Sign up to set email alerts
|

Absolute calibration of optical tweezers including aberrations

Abstract: We extend a previous proposal for absolute calibration of optical tweezers by including optical setup aberrations into the first-principles theory, with no fitting parameters. Astigmatism, the dominant term, is determined from images of the focused laser spot. Correcting it can substantially increase stiffness. Comparison with experimental results yields agreement within error bars for a broad range of bead sizes and trap heights, as well as different polarizations. Absolute calibration is established as a rel… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

2
22
0

Year Published

2014
2014
2024
2024

Publication Types

Select...
7
1
1

Relationship

2
7

Authors

Journals

citations
Cited by 27 publications
(24 citation statements)
references
References 12 publications
2
22
0
Order By: Relevance
“…The resulting Mie-Debye-spherical aberration (MDSA) theory of optical tweezers [34] thus accounts for the spherical aberration introduced by refraction at the glass slide. Excellent blind agreement with experiments was obtained when additional optical aberrations are also included [35,36]. Figure 3a shows the optical axial force Q z variation with the microsphere position z with respect to the focal point (normalized by the radius r = 1825 nm).…”
Section: Mie-debye Theory Of Optical Tweezerssupporting
confidence: 60%
“…The resulting Mie-Debye-spherical aberration (MDSA) theory of optical tweezers [34] thus accounts for the spherical aberration introduced by refraction at the glass slide. Excellent blind agreement with experiments was obtained when additional optical aberrations are also included [35,36]. Figure 3a shows the optical axial force Q z variation with the microsphere position z with respect to the focal point (normalized by the radius r = 1825 nm).…”
Section: Mie-debye Theory Of Optical Tweezerssupporting
confidence: 60%
“…Points corresponding to a given sample speed appear to be lumped together as expected. We measure α from a linear fit of all points corresponding to a given θ (the transverse stiffness can also be obtained and the results are compatible with theoretical MDSA+ values [29,30]). We intentionally rotate the camera by ∼ 30 o with respect to the axes of the microscope stage so as to have comparable displacements along x and y axes.…”
Section: Methodsmentioning
confidence: 62%
“…In order to trap small particles with optical tweezers, one has to consider the optical properties of the material that they are made, since these properties are critical to determine the resulting forces acting on the object. When subjected to a highly focused Gaussian light beam, dielectric particles are known to yield stable trapping in three dimensions, which was theoretically described by R. Gauthier, S. Wallace and A. Ashkin in geometrical optics regime [3,29] (λ << l, where λ is the laser wavelength and l is the particle radius), by J. Gordon in Rayleigh regime [30] (λ >> l), and by P. Maia Neto, H. Nussen- * Corresponding author: warlley.campos@ufv.br zveig and coworkers in Mie regime [31,32] (λ ∼ l). Metallic particles can be trapped under very special conditions.…”
Section: Introductionmentioning
confidence: 99%