Absolute calibration of small-angle neutron scattering data

Wignall, G. D.; Bates, Frank S.

doi:10.1107/s0021889887087181

Cited by 747 publications

(630 citation statements)

References 32 publications

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“…The scattering data were corrected for background and cell scattering, detector sensitivity, sample thickness, and transmission, and then converted to an absolute differential scattering cross section per unit sample volume (cm -1 ) using previously reported calibration techniques. 25 …”

Section: Methodsmentioning

confidence: 99%

Ternary Polymer Blends as Model Surfactant Systems

2000

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An investigation of the ternary phase prism for low molecular weight poly(ethylene oxide), squalane, and poly(ethylene oxide-b-ethylenepropylene) is presented. Comparisons are made between this ternary polymer system and classic water/oil/surfactant mixtures to establish further a universal phase diagram description for amphiphilic systems. A combination of visual isothermal measurements, small-angle X-ray scattering, smallangle neutron scattering, and dynamical mechanical spectrometry was used to characterize phases and determine phase boundaries. A rich phase diagram was revealed, including most of the equilibrium liquid crystalline phases associated with diblock copolymers, regions of two-phase and three-phase coexistence, and a bicontinuous microemulsion. Differences between this polymer phase diagram and those from water/oil/ surfactant systems highlight the strong effect of water in the latter.

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Section: Methodsmentioning

confidence: 99%

Ternary Polymer Blends as Model Surfactant Systems

2000

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“…31 Two instruments were used at the Institut Laue-Langevin. On D11, two sample-detector dis-tances of 1.2 and 8 m were used with collimation distances of 4 and 8 m. This gave an accessible Q-range of 0.004-0.3 Å −1 at λ = 10 Å.…”

Section: The Momentum Transfer Vector (Q) Is Defined In Equationmentioning

confidence: 99%

Interaction between Surfactants and Colloidal Latexes in Nonpolar Solvents Studied Using Contrast-Variation Small-Angle Neutron Scattering

Smith

Alexander²,

Brown³

et al. 2014

Langmuir

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The interaction between deuterium-labeled Aerosol OT surfactant (AOT-D 34 ) and stericallystabilized poly(methyl methacrylate) (PMMA) latex particles dispersed in nonpolar solvents has been studied using contrast-variation small-angle neutron scattering (CV-SANS). The electrophoretic mobilities (µ) of the latexes have been measured by phase-analysis light scattering, * To whom correspondence should be addressed † School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, United Kingdom

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“…The neutron wavelength λ was 8.1 Å (measured by routine time-of-flight experiments) with a wavelength resolution of 10% (full width at halfmaximum value). The two-dimensional isotropic scattering patterns were azimuthally averaged, converted to an absolute scale and corrected for detector efficiency by using the incoherent scattering pattern of pure water [27]. The background from the solvent and sample cell was subtracted from the raw patterns by conventional procedures [28].…”

Section: Sans Experimentsmentioning

confidence: 99%

Small angle neutron scattering as sensitive tool to detect ligand-dependent shape changes in a plant lectin with β-trefoil folding and their dependence on the nature of the solvent

André

Garamus

et al. 2008

Glycoconj J

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The galactoside-specific Viscum album L. agglutinin (VAA) is a potent biohazard akin to ricin and a mitogen for immune and tumor cells. These activities depend on cell surface binding to glycans. It is an open question whether the process of ligand binding alters the lectin's shape. Small angle neutron scattering (SANS) experiments revealed that the carbohydrate ligand lactose induced a decrease of the radius of gyration of dimeric VAA from 54.5±1 to 49.5±1 Å in water. Apparently, VAA in aqueous solution and at the concentrations tested at 3.6 mg/ml and above adopts a compacted structure as response to ligand binding. In contrast to the behavior in aqueous solution, lactose binding in DMSO resulted in an increase of the lectin's radius of gyration from 49±1 to 55.5±1 Å. Because shape changes may be reflected in the thermostability of the protein, this parameter was examined by activity assays of protein exposed to 60°C and 70°C and by differential scanning calorimetry (DSC). In line with the lactose-induced conformational alterations revealed by the SANS experiments, lactose presence enhanced the thermostability of VAA in water. Thus, binding of the carbohydrate ligand in solution can entail changes in shape and thermostability in the case of the tested plant lectin.

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Absolute calibration of small-angle neutron scattering data

Abstract: Absolute calibration forms a valuable diagnostic tool in small-angle neutron scattering (SANS) experiments,

Cited by 747 publications

References 32 publications

Ternary Polymer Blends as Model Surfactant Systems

Ternary Polymer Blends as Model Surfactant Systems

Interaction between Surfactants and Colloidal Latexes in Nonpolar Solvents Studied Using Contrast-Variation Small-Angle Neutron Scattering

Small angle neutron scattering as sensitive tool to detect ligand-dependent shape changes in a plant lectin with β-trefoil folding and their dependence on the nature of the solvent

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