Absolute Configuration of the New 3-epi-cladocroic Acid from the Mediterranean Sponge Haliclona fulva

Genta‐Jouve, Grégory; Thomas, Olivier P.

doi:10.3390/metabo3010024

Cited by 5 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The purification and characterization was carried out using our standard protocol (Cachet et al, 2009;Genta-Jouve and Thomas, 2013): after an organic extraction of the lyophilized material with CH 2 Cl 2 /MeOH (1:1), the extract was first submitted to fractionation by reversed phase (RP) Vacuum Liquid Chromatography (VLC). Fig.…”

Section: Resultsmentioning

confidence: 99%

Two-dimensional ultra high pressure liquid chromatography quadrupole/time-of-flight mass spectrometry for semi-targeted natural compounds identification

Genta‐Jouve

Croué

Weinberg³

et al. 2014

Phytochemistry Letters

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Two-dimensional ultra high pressure liquid chromatography quadrupole/time-of-flight mass spectrometry for semi-targeted natural compounds identification

Genta‐Jouve

Croué

Weinberg³

et al. 2014

Phytochemistry Letters

Self Cite

View full text Add to dashboard Cite

Stable and Enriched Cenarchaeum symbiosum and Uncultured Betaproteobacteria HF1 in the Microbiome of the Mediterranean Sponge Haliclona fulva (Demospongiae: Haplosclerida)

García-Bonilla¹,

Brandão²,

Pérez³

et al. 2018

Microb Ecol

View full text Add to dashboard Cite

Sponges harbor characteristic microbiomes derived from symbiotic relationships shaping their lifestyle and survival. Haliclona fulva is encrusting marine sponge species dwelling in coralligenous accretions or semidark caves of the Mediterranean Sea and the near Atlantic Ocean. In this work, we characterized the abundance and core microbial community composition found in specimens of H. fulva by means of electron microscopy and 16S amplicon Illumina sequencing. We provide evidence of its low microbial abundance (LMA) nature. We found that the H. fulva core microbiome is dominated by sequences belonging to the orders Nitrosomonadales and Cenarchaeales. Seventy percent of the reads assigned to these phylotypes grouped in a very small number of high-frequency operational taxonomic units, representing niche-specific species Cenarchaeum symbiosum and uncultured Betaproteobacteria HF1, a new eubacterial ribotype variant found in H. fulva. The microbial composition of H. fulva is quite distinct from those reported in sponge species of the same Haliclona genus. We also detected evidence of an excretion/capturing loop between these abundant microorganisms and planktonic microbes by analyzing shifts in seawater planktonic microbial content exposed to healthy sponge specimens maintained in aquaria. Our results suggest that horizontal transmission is very likely the main mechanism for symbionts' acquisition by H. fulva. So far, this is the first shallow water sponge species harboring such a specific and predominant assemblage composed of these eubacterial and archaeal ribotypes. Our data suggests that this symbiotic relationship is very stable over time, indicating that the identified core microbial symbionts may play key roles in the holobiont functioning.

show abstract

Metabolome variability for two Mediterranean sponge species of the genus Haliclona: specificity, time, and space

et al. 2018

View full text Add to dashboard Cite

In this study, the intraspecific (temporal and spatial) variability of two Mediterranean Haliclona sponges (H. fulva and H. mucosa) was investigated through an untargeted and then targeted metabolomics approach and further compared to their interspecific variability. Methods Samples of both species were collected monthly during 1 year in the coralligenous habitat of the Northwestern Mediterranean sae at Marseille and Nice. Their metabolomic profiles were obtained by UHPLC-QqToF analyses. Results Marked variations were noticed in April and May for both species including a decrease in Shannon's diversity and concentration in specialized metabolites together with an increase in fatty acids and lyso-PAF like molecules. Spatial variations across different sampling sites could also be observed for both species, however in a lesser extent. Conclusions Synchronous metabolic changes possibly triggered by physiological factors like reproduction and/or environmental factors like an increase in the water temperature were highlighted for both Mediterranean Haliclona species inhabiting close habitats but displaying different biosynthetic pathways. Despite significative intraspecific variations, metabolomic variability remains minor when compared to interspecific variations for these congenerous species, therefore suggesting the predominance of genetic information of the holobiont in the observed metabolome.

show abstract

Absolute Configuration of the New 3-epi-cladocroic Acid from the Mediterranean Sponge Haliclona fulva

Cited by 5 publications

References 27 publications

Two-dimensional ultra high pressure liquid chromatography quadrupole/time-of-flight mass spectrometry for semi-targeted natural compounds identification

Two-dimensional ultra high pressure liquid chromatography quadrupole/time-of-flight mass spectrometry for semi-targeted natural compounds identification

Stable and Enriched Cenarchaeum symbiosum and Uncultured Betaproteobacteria HF1 in the Microbiome of the Mediterranean Sponge Haliclona fulva (Demospongiae: Haplosclerida)

Metabolome variability for two Mediterranean sponge species of the genus Haliclona: specificity, time, and space

Contact Info

Product

Resources

About