Absolute estimates of larval populations of culicid mosquitoes: comparison of ‘capture‐recapture’, ‘removal’ and ‘dipping’ methods

Croset, H.; Papierok, B.; Rioux, J. A.; Gabinaud, A.; Cousserans, J.; Arnaud, D

doi:10.1111/j.1365-2311.1976.tb01229.x

Cited by 18 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Stable isotope enrichment of natural mosquito larval sites potentially enables large‐scale marking of wild mosquitoes by continuously and non‐invasively marking newly hatched larvae in situ. Unlike radioactive isotopes (Baldwin, James, & Welch, ; Croset et al, ; Papierok, Croset, & Rioux, ; , ), stable isotopes do not emit radiation or decay, occur naturally in the environment including in our food, and are considered safe for all organisms (including humans) and the environment (Hood‐Nowotny, ). Mosquito larvae that develop in water enriched with stable isotopes yield adults that are potentially marked for life with an elevated ratio of the enriched “ heavy” to lighter isotope (Hamer et al, , ; Hood‐Nowotny & Knols, ), providing opportunities to test hypotheses such as local persistence of Anopheles coluzzii through the dry season via aestivation (dormancy).…”

Section: Introductionmentioning

confidence: 99%

Marking mosquitoes in their natural larval sites using ²H‐enriched water: A promising approach for tracking over extended temporal and spatial scales

et al. 2019

View full text Add to dashboard Cite

Tracking mosquitoes using current methods of mark–release–recapture are limited to small spatial and temporal scales exposing major gaps in understanding long‐range movements and extended survival. Novel approaches to track mosquitoes may yield fresh insights into their biology which improves intervention activities to reduce disease transmission. Stable isotope enrichment of natural mosquito breeding sites allows large‐scale marking of wild mosquitoes absent human handling. Mosquito larvae that develop in 2H‐enriched water are expected to be detectable for over 4 months using tissue mass fraction 2H measurements, providing opportunities for long‐term mark–capture studies on a large scale. A laboratory study followed by a field experiment of mosquito larval habitat 2H enrichment was conducted in Mali, to evaluate potential labelling of wild mosquitoes. Twelve natural larval sites were enriched using [2H] deuterium oxide (D2O, 99%). Enrichment level was maintained by supplementation following dilution by rains. Availability of 2H to mosquito larvae was enhanced by locally collected and cultured microorganisms (i.e. protozoa, algae and bacteria) reared in deuterated water, and provided as larval diet. Putative natural predators were removed from the larval sites and first instar larvae Anopheles gambiae s.l. larvae were added every other day. Emergence traps enabled collection of eclosing adults. Adult mosquitoes were kept at laboratory conditions for analysis of label attrition with age. Deuterium enrichment of wild mosquitoes above background levels (maximum = 143.1 ppm) became apparent 5–6 days after initial exposure, after which 2H values increased steadily until c. 24 days later (to a mean of c. 220 ppm). Anopheles and Culex mosquitoes showed significantly different 2H values (211 and 194.2 ppm, respectively). Both genera exhibited exponential label attrition (e(‐x)) amounting to 21.6% by day 30 post‐emergence, after which attrition rate continuously decreased. Males of both taxa exhibited a higher mean 2H value compared to females. Deuterium oxide proved useful in marking mosquitoes in their natural larval sites and although costly, may prove valuable for studies of mosquitoes and other aquatic insects. Based on our field study, we provide a protocol for marking mosquito larval sites using deuterium oxide.

show abstract

Section: Introductionmentioning

confidence: 99%

Marking mosquitoes in their natural larval sites using ²H‐enriched water: A promising approach for tracking over extended temporal and spatial scales

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Although a variety of samplers have been described (e.g. Macan, 1958;Cummins, 1962;Hilsenhoff, 1969;Maitland, 1969;Crossman & Cairns, 1974;Croset et al, 1976;Elliott & Tullet, 1978), the pond net remains the most popular device employed in freshwaters. While procedures have been described for pond net sampling of lotic systems (SAC, 1978;I.S.O., 1985) no standardised technique has been designed for lentic systems.…”

Section: Introductionmentioning

confidence: 99%

A Comparison of the Efficacy of Pond-Net and Box Sampling Methods in Turloughs – Irish Ephemeral Aquatic Systems

et al. 2004

View full text Add to dashboard Cite

Two methods were used to sample aquatic macroinvertebrates in three turloughs. Turloughs are systems that flood periodically from groundwater, in response to local rainfall patterns and contain rare aquatic species and assemblages. The first method used a standard pond net that was swept through the water column, while the second involved fixing a rectangular, open-bottomed box to the substrata and removing all organisms with a net. Similar overall sampling effort was applied to each method and individual box samples were found to take longer to gather than pond net samples. The box method, however, gave the maximum yield for a given sampling effort. Significantly more beetle species and individuals were recorded per unit area of bottom at all three turloughs using the box method. Multivariate analysis segregated samples, firstly according to site and secondly, with respect to method. The box method is a viable alternative to sampling with a pond net. It is more quantitative, objective, specific and reliable. This is particularly important in habitats distinguished by rare species and assemblages, and for which monitoring is driven by legislative needs.

show abstract

“…The total population of larvae (P) in each habitat was calculated by the following formula. P = vn/c, where c = water holding capacity of the ladle, v = the volume of the water contained in the pool, n = mean number of larvae per dip [ 13 ].Larval population was estimated for each habitat by MSEXCEL 2007.…”

Section: Methodsmentioning

confidence: 99%

In target areas where human mosquito-borne diseases are diagnosed, the inclusion of the pre-adult mosquito aquatic niches parameters will improve the integrated mosquito control program

Ghosh¹,

Podder²,

Panja³

et al. 2020

PLoS Negl Trop Dis

View full text Add to dashboard Cite

In human communities inhabiting areas–such as West Bengal- India–where perpetuate the pre-imago & adult developmental stages of mosquitoes; many infectious diseases are still diagnosed such as Dengue, Malaria and Acute Encephalitis Syndrome. The control of the aquatic developmental stages is one of the easiest way to prevent the emergence of adults—the blood feeding adult females being thus prevented to sample their blood meal and to lay their eggs in the aquatic milieu where develop the aquatic pre-imaginal developmental stages. Moreover, reducing the adult population size also the probability of for the blood feeding adult female mosquitoes to act as hosts and vectors of the arboviruses such as dengue virus & Japanese encephalitis virus as well as of Plasmodium . Several environmental factors including water quality parameters are responsible for the selection of oviposition sites by the female mosquitoes. In our study, larval densities of three important mosquitoes ( Aedes / A . albopictus , Anopheles / An . stephensi and Culex / C . vishnui ) were measured and water qualities of their habitat i.e. pH, Specific Conductance, Dissolved Oxygen, Chemical Oxygen Demand, Total alkalinity (T alk ), Hardness, Nitrate nitrogen and Ammonia nitrogen were analyzed in 2017 and 2018 in many districts of West Bengal where humans beings are suffering from arboviruses and /or malaria. Whereas we have found positive correlation of density of C. vishnui and A . albopictus with the water factors except Chemical Oxygen Demand (COD) and T alk , for An . stephensi all these factors except pH, COD and T alk have positive correlation. Hardness of the water shows positive correlation with the density of An . stephensi and C . vishnui but negative correlation with density of A . albopictus . Contour plot analysis demonstrates that occurrence of each mosquito species lies in between specific range of water factors. Inter- correlation analysis revealed that mosquitoes were negatively correlated with each other. A positive correlation of the water quality parameters and larval density, over two successive years, was also noticed. In conclusion, the increasing level of pollution due to industrial and other irresponsible waste management system which changes the water quality parameters may also influence mosquito population.

show abstract

Absolute estimates of larval populations of culicid mosquitoes: comparison of ‘capture‐recapture’, ‘removal’ and ‘dipping’ methods

Cited by 18 publications

References 8 publications

Marking mosquitoes in their natural larval sites using ²H‐enriched water: A promising approach for tracking over extended temporal and spatial scales

Marking mosquitoes in their natural larval sites using ²H‐enriched water: A promising approach for tracking over extended temporal and spatial scales

A Comparison of the Efficacy of Pond-Net and Box Sampling Methods in Turloughs – Irish Ephemeral Aquatic Systems

In target areas where human mosquito-borne diseases are diagnosed, the inclusion of the pre-adult mosquito aquatic niches parameters will improve the integrated mosquito control program

Contact Info

Product

Resources

About

Absolute estimates of larval populations of culicid mosquitoes: comparison of ‘capture‐recapture’, ‘removal’ and ‘dipping’ methods

Cited by 18 publications

References 8 publications

Marking mosquitoes in their natural larval sites using 2H‐enriched water: A promising approach for tracking over extended temporal and spatial scales

Marking mosquitoes in their natural larval sites using 2H‐enriched water: A promising approach for tracking over extended temporal and spatial scales

A Comparison of the Efficacy of Pond-Net and Box Sampling Methods in Turloughs – Irish Ephemeral Aquatic Systems

In target areas where human mosquito-borne diseases are diagnosed, the inclusion of the pre-adult mosquito aquatic niches parameters will improve the integrated mosquito control program

Contact Info

Product

Resources

About

Marking mosquitoes in their natural larval sites using ²H‐enriched water: A promising approach for tracking over extended temporal and spatial scales

Marking mosquitoes in their natural larval sites using ²H‐enriched water: A promising approach for tracking over extended temporal and spatial scales