A high-accuracy Global Ionosphere Model (GIM) is significant for precise positioning and navigating with the Global Navigation Satellite System (GNSS), as well as space weather applications.To obtain a precise GIM, it is critical to take both the ionospheric observable and mathematical model into consideration. In this contribution, the undifferenced ambiguity-fixed carrier-phase ionospheric observable is first determined from a global distribution of permanent receivers. Accuracy assessment with a co-located station experiment shows that the observational errors affecting the ambiguity-fixed carrier-phase ionospheric observables range from 0.10 to 0.35 Total Electron Content Units (TECUs, where 1 TECU = 10 16 e − /m 2 and corresponds to 0.162 m on the Global Positioning System, GPS L1 frequency), indicating that the ambiguity-fixed carrier-phase ionospheric observable is over one order of magnitude more accurate than the carrier-phase leveled-code one (from 1.21 to 3.77 TECUs). Second, to better model the structure of the ionosphere, a two-layer GIM has been built based on the above carrier-phase observable. Preliminary global accuracy evaluation demonstrates that the accuracy of the two-layer GIM is below 1 TECU and about 2 TECUs during low and high solar activity periods. Third, the single-frequency point positioning experiment is adopted to test the ionosphere mitigation effects of the GIMs. Positioning results demonstrate that the single-frequency positioning accuracy can be improved by more than 30% using the undifferenced ambiguity-fixed ionospheric observable-derived two-layer GIM, compared with that using the carrier-phase leveled-code ionospheric observable-based single-layer GIM.