Absorption, Action, and Translocation of Glyphosate

Sprankle, Paul; Meggitt, W. F.; Penner, Donald

doi:10.1017/s0043174500052930

Cited by 224 publications

(164 citation statements)

References 2 publications

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“…In contrast, in the S and in the SF populations, a greater percentage of 14 C-glyphosate moved to non-treated leaves and the stem. Because glyphosate tends to be actively phloem transported and accumulates in meristematic tissue (Sprankle et al 1975;McWhorter et al 1980;Arnaud et al 1994), the different translocation pattern in the OR population and other L. rigidum populations is associated with glyphosate resistance. 14 C-glyphosate translocation patterns between the SF and the S populations were similar at 48 h but different at 24 and 72 h after treatment.…”

Section: Figmentioning

confidence: 99%

Investigating the mechanisms of glyphosate resistance in Lolium multiflorum

Perez‐Jones

Park

Polge

et al. 2007

Planta

165

191

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Evolved resistance to the herbicide glyphosate has been reported in eleven weed species, including Lolium multiflorum. Two glyphosate-resistant L. multiflorum populations were collected, one from Chile (SF) and one from Oregon, USA (OR), and the mechanisms conferring glyphosate resistance were studied. Based on a Petri dish dose-response bioassay, the OR and the SF populations were two and fivefold more resistant to glyphosate when compared to the susceptible (S) population, respectively; however, based on a whole-plant dose-response bioassay, both OR and SF populations were fivefold more resistant to glyphosate than the S population, implying that different resistance mechanisms might be involved. The S population accumulated two and three times more shikimic acid in leaf tissue 96 h after glyphosate application than the resistant OR and SF populations, respectively. There were no differences between the S and the glyphosate-resistant OR and SF populations in 14C-glyphosate leaf uptake; however, the patterns of 14C-glyphosate translocation were significantly different. In the OR population, a greater percentage of 14C-glyphosate absorbed by the plant moved distal to the treated section and accumulated in the tip of the treated leaf. In contrast, in the S and in the SF populations, a greater percentage of 14C-glyphosate moved to non-treated leaves and the stem. cDNA sequence analysis of the EPSP synthase gene indicated that the glyphosate-resistant SF population has a proline 106 to serine amino acid substitution. Here, we report that glyphosate resistance in L. multiflorum is conferred by two different mechanisms, limited translocation (nontarget site-based) and mutation of the EPSP synthase gene (target site-based).

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Section: Figmentioning

confidence: 99%

Investigating the mechanisms of glyphosate resistance in Lolium multiflorum

Perez‐Jones

Park

Polge

et al. 2007

Planta

165

191

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“…Therefore, a possible internal inactivation of micronutrients in young leaves via formation of glyphosatemetal complexes, unavailable for plant metabolism, was also investigated. The well-documented ability of glyphosate to form stable complexes with metal cations such as Al, Fe, Zn, Mn and Ca (Sprankle et al 1975) may thereby induce internal micronutrient deficiencies, although total micronutrient leaf concentrations are in the sufficiency range. However, micronutrients in the 80% ethanol-soluble LMW fraction of young leaves obtained from glyphosate-treated and non-treated control plants in soil culture were not significantly different (Table 2).…”

Section: Plant Growthmentioning

confidence: 99%

“…To assess a possible physiological immobilization of the investigated micronutrients in young leaves of glyphosate-treated plants by metal complexation with glyphosate (Sprankle et al 1975), leaf tissue was extracted with 80% ethanol to separate the lowmolecular weight (LMW) soluble fraction containing potential metal complexes with glyphosate, from highmolecular weight (HMW) compounds. After glyphosate application, the formation of stable LMW metal complexes with glyphosate may limit the availability of micronutrients for interactions in the HMW fraction.…”

mentioning

confidence: 99%

Glyphosate-induced impairment of plant growth and micronutrient status in glyphosate-resistant soybean (Glycine max L.)

et al. 2008

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This investigation demonstrated potential detrimental side effects of glyphosate on plant growth and micronutrient (Mn, Zn) status of a glyphosate-resistant (GR) soybean variety (Glycine max cv. Valiosa), which were found to be highly dependent on the selected growth conditions. In hydroponic experiments with sufficient Mn supply [0.5 μM], the GR cv. Valiosa produced similar plant biomass, root length and number of lateral roots in the control treatment without glyphosate as compared to its non-GR parental line cv. Conquista. However, this was associated with 50% lower Mn shoot concentrations in cv. Conquista, suggesting a higher Mn demand of the transgenic cv. Valiosa under the selected growth conditions. Glyphosate application significantly inhibited root biomass production, root elongation, and lateral root formation of the GR line, associated with a 50% reduction of Mn shoot concentrations. Interestingly, no comparable effects were detectable at low Mn supply [0.1 μM]. This may indicate Mn-dependent differences in the intracellular transformation of glyphosate to the toxic metabolite aminomethylphosphonic acid (AMPA) in the two isolines. In soil culture experiments conducted on a calcareous loess sub-soil of a Luvisol (pH 7.6) and a highly weathered Arenosol (pH 4.5), shoot biomass production and Zn leaf concentrations of the GR-variety were affected by glyphosate applications on the Arenosol but not on the calcareous Loess sub-soil. Analysis of micronutrient levels in high and low molecular weight (LMW) fractions (80% ethanol extracts) of young leaves revealed no indications for internal immobilization of micronutrients (Mn, Zn, Fe) by excessive complexation with glyphosate in the LMW phase.

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“…Glyphosate is a systemic herbicide that is first absorbed by foliage and then translocated throughout the plant via the phloem and further transported to metabolic sinks such as meristems and roots. Rapid translocation of 14 Cglyphosate in quackgrass [Elymus repens (L.) Gould] was first reported by Sprankle et al (1975a). A few years later Coupland and Caseley (1979) noticed that significant amounts of 14 C-glyphosate were exuded from intact roots of quackgrass into a surrounding solution.…”

mentioning

confidence: 99%

Glyphosate and phosphorus leaching and residues in boreal sandy soil

et al. 2009

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Glyphosate [(N-(phosphonomethyl)glycine)] is a widely used herbicide and it is known to compete for the same sorption sites in soil as phosphorus. Persistence and losses of glyphosate were monitored in a field with low phosphorus status and possible correlation between glyphosate and phosphorus leaching losses was studied. Glyphosate and its metabolite AMPA (aminomethyl phosphonic acid) residues in soil samples were analysed after a single application in autumn. Twenty months after the application the residues of glyphosate and AMPA in the topsoil (0-25 cm) corresponded to 19% and 48%, respectively, of the applied amount of glyphosate, and traces of glyphosate and AMPA residues were detected in deeper soil layers (below 35 cm). These results indicate rather long persistence for glyphosate in boreal soils. Surface runoff and subsurface drainflow were collected continuously all year round for 20 months and analysed for glyphosate, AMPA, dissolved phosphate, total phosphorus and total suspended solids.The glyphosate concentrations in the surface runoff water were highest, with 99% of the total leaching losses obtained, during the periods of snow melting and soil thawing in the first winter following the autumn application. The total leaching of glyphosate was 5.12 g ha −1 and that of AMPA 0.48 g ha −1 , corresponding to about 0.51% and 0.07%, respectively, of the applied amount of glyphosate. No residues of glyphosate and AMPA were detected in the subsurface drainflow. The correlations between concentrations of glyphosate and dissolved orthophosphate as well as glyphosate and total phosphorus in surface runoff were significant (p<0.01).

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Absorption, Action, and Translocation of Glyphosate

Cited by 224 publications

References 2 publications

Investigating the mechanisms of glyphosate resistance in Lolium multiflorum

Investigating the mechanisms of glyphosate resistance in Lolium multiflorum

Glyphosate-induced impairment of plant growth and micronutrient status in glyphosate-resistant soybean (Glycine max L.)

Glyphosate and phosphorus leaching and residues in boreal sandy soil

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