Absorption and efflux of glyphosate by cell suspensions

Hetherington, Paul; Marshall, G.; Kirkwood, R. C.; Warner, Jorge

doi:10.1093/jxb/49.320.527

Cited by 42 publications

(9 citation statements)

References 23 publications

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“…As shown in previous studies (Denis and Delrot 1993;Hetherington et al 1998;Morin et al 1997;Shaner 2009), glyphosate, as a phosphonate, seems to use the same phosphatespecific transport systems as inorganic phosphorus. Not only glyphosate uptake was shown to be inhibited by sodium phosphate and phosphonoformic acid, but also by PCMBS (p-chromercuribenzenesulfonic acid) and DCCD (N,N 0 -dicyclohexylcarbodiimide) (Morin et al 1997).…”

Section: Discussionsupporting

confidence: 59%

“…There appear to be at least two mechanisms of glyphosate uptake (active and passive) in plants; however, they are not well understood to date. An active system operates at low concentrations of herbicide and may presumably involve a phosphate transporter since it has been shown that the glyphosate uptake is inhibited by sodium phosphate and phosphonoformic acid, competitive inhibitors of phosphate assimilation in plant cells (Denis and Delrot 1993;Hetherington et al 1998;Morin et al 1997;Shaner 2009). The interruption of essential metabolic pathways manifests as yellowing and wilting of the plant leaves followed by browning.…”

Section: Introductionmentioning

confidence: 99%

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Expression of miRNAs involved in phosphate homeostasis and senescence is altered in glyphosate-treated maize

et al. 2015

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Glyphosate is a systemic, nonselective and most widely used herbicide in the world. The introduction of glyphosate-resistant crops in the mid-1990s resulted in a dramatic increase in the use of glyphosate herbicide making it most widely used herbicide in the world. The average maize yield loss in the field caused by pests is around 20 % but in many regions it is much higher. It is now clear that glyphosate causes broader range of physiological alterations than previously assumed and some plants gain higher level of resistance to glyphosate without the need to use genetic engineering methods. To understand the mechanisms of such heightened resistance we must first know the processes mediating the plants' death in response to glyphosate treatment. Here, we show that 12 miRNAs, belonging to miR167, miR396, miR159, miR156, miR169, miR444 and miR827 families, are significantly upregulated, and one, miR166, downregulated following glyphosate treatment. These miRNAs have been previously shown to be involved in abiotic stress responses and implicated in senescence. Strikingly, two of the induced miRNAs, miR444 and miR827, have been shown to regulate phosphate transport pathways, which seem to be common for Pi and glyphosate uptake.

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Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Expression of miRNAs involved in phosphate homeostasis and senescence is altered in glyphosate-treated maize

et al. 2015

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“…Moreover, recently, single nucleotide polymophisms (SNPs) and indels were detected in genes encoding phosphate transporters in TL and SL Z. mays lines (Mahmoud et al 2020). This is particularly important, since glyphosate has been shown to be recognized by phosphate transporters (Hetherington et al 1998). Notably, phosphate transporters 1 and 2 were implicated in the active transport of glyphosate into plant cells (Mahmoud et al 2020;Denis and Delrot 1993;Hetherington et al 1998;Morin et al 1997;Shaner 2009;Gomes et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance

et al. 2021

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DNA methylation plays a crucial role in the regulation of gene expression, activity of transposable elements, defense against foreign DNA, and inheritance of specific gene expression patterns. The link between stress exposure and sequence-specific changes in DNA methylation was hypothetical until it was shown that stresses can induce changes in the gene expression through hypomethylation or hypermethylation of DNA. To detect changes in DNA methylation under herbicide stress in two local Zea mays inbred lines exhibiting differential susceptibility to Roundup®, the methylation-sensitive amplified polymorphism (MSAP) technique was used. The overall DNA methylation levels were determined at approximately 60% for both tested lines. The most significant changes were observed for the more sensitive Z. mays line, where 6 h after the herbicide application, a large increase in the level of DNA methylation (attributed to the increase in fully methylated bands (18.65%)) was noted. DNA sequencing revealed that changes in DNA methylation profiles occurred in genes encoding heat shock proteins, membrane proteins, transporters, kinases, lipases, methyltransferases, zinc-finger proteins, cytochromes, and transposons. Herbicide stress-induced changes depended on the Z. mays variety, and the large increase in DNA methylation level in the sensitive line resulted in a lower ability to cope with stress conditions.

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“…A modification in a phosphate carrier protein has been proposed as a resistance mechanism to glyphosate (Shaner, 2009;Roso and Vidal, 2010). It has been shown that glyphosate does not readily move across a laboratory made semi-permeable membrane (Takano et al, 2019) and cellular uptake may be inhibited in the presence of phosphate (Hetherington et al, 1998). These results provide evidence that glyphosate is taken up by the cell through a phosphate transporter.…”

Section: Resistance To Glyphosatementioning

confidence: 93%

“…It inhibits EPSPS, preventing biosynthesis of aromatic amino acids for plant metabolism (Shaner, 2014). Glyphosate uptake by plant cells may be active or passive, with the active uptake being facilitated by membranebound phosphate transporters (Hetherington et al, 1998). Several properties make glyphosate a unique and important tool: it is a non-selective, systemic, slow-acting, post-emergence, and relatively non-expensive herbicide (Duke et al, 2018).…”

Section: Resistance To Glyphosatementioning

confidence: 99%

Non-target-Site Resistance in Lolium spp. Globally: A Review

et al. 2021

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The Lolium genus encompasses many species that colonize a variety of disturbed and non-disturbed environments. Lolium perenne L. spp. perenne, L. perenne L. spp. multiflorum, and L. rigidum are of particular interest to weed scientists because of their ability to thrive in agricultural and non-agricultural areas. Herbicides are the main tool to control these weeds; however, Lolium spp. populations have evolved multiple- and cross-resistance to at least 14 herbicide mechanisms of action in more than 21 countries, with reports of multiple herbicide resistance to at least seven mechanisms of action in a single population. In this review, we summarize what is currently known about non-target-site resistance in Lolium spp. to acetyl CoA carboxylase, acetohydroxyacid synthase, microtubule assembly, photosystem II, 5-enolpyruvylshikimate-3-phosphate synthase, glutamine synthetase, very-long chain fatty acids, and photosystem I inhibitors. We suggest research topics that need to be addressed, as well as strategies to further our knowledge and uncover the mechanisms of non-target-site resistance in Lolium spp.

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Absorption and efflux of glyphosate by cell suspensions

Cited by 42 publications

References 23 publications

Expression of miRNAs involved in phosphate homeostasis and senescence is altered in glyphosate-treated maize

Expression of miRNAs involved in phosphate homeostasis and senescence is altered in glyphosate-treated maize

Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance

Non-target-Site Resistance in Lolium spp. Globally: A Review

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