Absorption edge subtraction imaging for volumetric measurement in an animal model of malignant brain tumor

Rigley, S; Rigon, Luigi; Ataelmannan, K; Chapman, Dean; Doucette, R.; Griebel, Robert; Juurlink, Bernhard H.J.; Arfelli, Fulvia; Menk, R.H.; Tromba, Giuliana; Barroso, R.C.; Beveridge, Toby Ean; Lewis, Robert A.; Pavlov, Konstantin Mikhailovitch; Siu, Karen Kit Wan; Hall, Chris; Schültke, Elisabeth

doi:10.1016/j.nima.2005.03.072

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…59 Cell tracking using ex-vivo labeled cells with CT has been demonstrated with a number of applications including T-cell labeling for cancer, C6 glioma cells for brain tumor models, olfactory ensheathing cells for spinal compression injury models and pancreatic islets labeling for diabetes. 14, 60–64 In-situ labeling, where nanoparticles are injected into animals and cells take up the nanoparticles in vivo , is more straightforward and simpler to implement clinically. 65–66 However, in-situ labeling may result in labeling a variety of cells, such as macrophages and monocytes resident within the plaque, as well as circulating monocytes, confounding results from imaging.…”

Section: Discussionmentioning

confidence: 99%

Labeling monocytes with gold nanoparticles to track their recruitment in atherosclerosis with computed tomography

et al. 2016

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Monocytes are actively recruited from the circulation into developing atherosclerotic plaques. In the plaque, monocytes differentiate into macrophages and eventually form foam cells. Continued accumulation of foam cells can lead to plaque rupture and subsequent myocardial infarction. X-ray computed tomography (CT) is the best modality to image the coronary arteries non-invasively, therefore we have sought to track the accumulation of monocytes into atherosclerotic plaques using CT. Gold nanoparticles were synthesized and stabilized with a variety of ligands. Select formulations were incubated with an immortalized monocyte cell line in vitro and evaluated for cytotoxicity, effects on cytokine release, and cell uptake. These data identified a lead formulation, 11-MUDA capped gold nanoparticles, to test for labeling primary monocytes. The formulation did not the affect the viability or cytokine release of primary monocytes and was highly taken up by these cells. Gold labeled primary monocytes were injected into apolipoprotein E deficient mice kept on Western diet for 10 weeks. Imaging was done with a microCT scanner. A significant increase in attenuation was measured in the aorta of mice receiving the gold labeled cells as compared to control animals. Following the experiment, the biodistribution of gold was evaluated in major organs. Additionally, plaques were sectioned and examined with electron microscopy. The results showed that gold nanoparticles were present inside monocytes located within plaques. This study demonstrates the feasibility of using gold nanoparticles as effective cell labeling contrast agents for non-invasive imaging of monocyte accumulation within plaques with CT.

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Section: Discussionmentioning

confidence: 99%

Labeling monocytes with gold nanoparticles to track their recruitment in atherosclerosis with computed tomography

et al. 2016

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“…Thus, the contribution of absorption edges to x-ray attenuation is greatest when the mean energy of the source spectrum is near the contrast agent K-edge. Moreover, absorption edge subtraction can be used to increase the signal-to-noise ratio by subtracting images taken at energy levels above and below the K-edge of a material or contrast agent [33].…”

Section: Contrast-enhancementmentioning

confidence: 99%

“…AuNPs have been surface functionalized with peptides [21,72], antibodies [23,47,[63][64][69][70], saccharides [20,48,[66][67], lipoproteins [22,132] and numerous small molecules [32,61,71,73,[122][123]125,[129][130][131] for active targeting. Molecular ligands can be strategically chosen for target-specific binding to a site of interest, including cancerous cells or tumors [21,33,[47][48]61,[63][64][65][66][67][68][69][70][71][72][122][123][124][125], the liver [20], lymph nodes [23], atherosclerotic plaque [22] and bone or mineral deposits [32,60,73,…”

Section: Surface Functionalizationmentioning

confidence: 99%

Gold Nanoparticles as Contrast Agents in x-ray Imaging and Computed Tomography

Cole

Ross

Tilley

et al. 2015

Nanomedicine (Lond.)

303

212

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Computed tomography enables 3D anatomic imaging at a high spatial resolution, but requires delivery of an x-ray contrast agent to distinguish tissues with similar or low x-ray attenuation. Gold nanoparticles (AuNPs) have gained recent attention as an x-ray contrast agent due to exhibiting a high x-ray attenuation, nontoxicity and facile synthesis and surface functionalization for colloidal stability and targeted delivery. Potential diagnostic applications include blood pool imaging, passive targeting and active targeting, where actively targeted AuNPs could enable molecular imaging by computed tomography. This article summarizes the current state of knowledge for AuNP x-ray contrast agents within a paradigm of key structure-property-function relationships in order to provide guidance for the design of AuNP contrast agents to meet the necessary functional requirements in a particular application. Functional requirements include delivery to the site of interest (e.g., blood, tumors or microcalcifications), nontoxicity during delivery and clearance, targeting or localization at the site of interest and contrast enhancement for the site of interest compared with surrounding tissues. Design is achieved by strategically controlling structural characteristics (composition, mass concentration, size, shape and surface functionalization) for optimized properties and functional performance. Examples from the literature are used to highlight current design trade-offs that exist between the different functional requirements.

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“…Qualitative studies will be reported, for the first time, with a sample of ox brain tissue. The use of brain tissue with ABI has recently been applied to analysis of tumor in mouse models (Mannan et al, 2005;Rigley et al, 2005). Perhaps together with computed tomography, at higher energies, ABI could be useful as an alternative method for the diagnosis of brain illness without any use of contrast agents.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and qualitative studies on high-contrast X-ray radiography with an asymmetrical crystal set-up at Elettra

Hönnicke

Rigon

Menk

et al. 2005

J Synchrotron Radiat

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An analyzer-based X-ray phase contrast imaging (ABI) system with an asymmetrical crystal set-up was mounted at Elettra's SYRMEP beamline. It was the first time that this set-up was implemented at Elettra. Advantages and disadvantages of such a set-up were exploited with quantitative and qualitative studies. For quantitative studies a simple hollow tube and strands were used as samples. Qualitative studies were developed using this technique with ox brain tissue, which has never been previously examined in this manner. Minute details were found in the ABIs and in the diffraction enhanced images. This could indicate the possibility of using these techniques with computerized tomography in the future as an auxiliary method in diagnosing brain illnesses such as Alzheimer and Creutzfeldt-Jacob disease. Propagation-based X-ray phase contrast imaging was also qualitatively exploited and compared with ABI using a Loxosceles gaucho spider as a sample.

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Absorption edge subtraction imaging for volumetric measurement in an animal model of malignant brain tumor

Cited by 5 publications

References 7 publications

Labeling monocytes with gold nanoparticles to track their recruitment in atherosclerosis with computed tomography

Labeling monocytes with gold nanoparticles to track their recruitment in atherosclerosis with computed tomography

Gold Nanoparticles as Contrast Agents in x-ray Imaging and Computed Tomography

Quantitative and qualitative studies on high-contrast X-ray radiography with an asymmetrical crystal set-up at Elettra

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