Absorption of insulin from Pluronic F-127 gels following subcutaneous administration in rats

Barichello, José Mário; Morishita, Mariko; Takayama, K.; Nagai, Tsuneji

doi:10.1016/s0378-5173(99)00119-2

Cited by 202 publications

(111 citation statements)

References 31 publications

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“…0.99) (Figure 4). Similar zero-order release kinetics could be found in previous reports for recombinant hirudin, 57 interleukin-2, 58 insulin, 43 and ceftiofur. 59 However, first-order kinetics and Higuchi kinetics have also been reported.…”

Section: Correlation Between Gel Dissolution and Drug Releasesupporting

confidence: 73%

“…It also has been used in rectal, ophthalmic, parenteral, and nasal administrations, as well as in subcutaneous administrations. 42,43 For localized cancer therapy, intratumoral or peritumoreal injection of this gel will lead to the formation of a 'depot' at the site of administration that slowly and continuously releases the drug to the tumor and surrounding tissue. Using a topical or injectable gel for physical targeting has additional advantages over passive or other actively targeted therapies in that it can deliver a drug throughout the tumor regardless of vascular status, thus providing accurate dosing without systemic toxicity.…”

Section: Introductionmentioning

confidence: 99%

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Thermoreversible Pluronic® F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies

Nie¹,

Hsiao²,

Pan³

et al. 2011

IJN

119

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Purpose To develop an in situ gel system comprising liposome-containing paclitaxel (PTX) dispersed within the thermoreversible gel (Pluronic ® F127 gel) for controlled release and improved antitumor drug efficiency. Methods The dialysis membrane and membrane-less diffusion method were used to investigate the in vitro drug release behavior. Differential scanning calorimetry (DSC) thermal analysis was used to investigate the “micellization” and “sol/gel transition” process of in situ gel systems. In vitro cytotoxicity and drug uptake in KB cancer cells were determined by MTT, intercellular drug concentration, and fluorescence intensity assay. Results The in vitro release experiment performed with a dialysis membrane model showed that the liposomal gel exhibited the longest drug-release period compared with liposome, general gel, and commercial formulation Taxol ® . This effect is presumably due to the increased viscosity of liposomal gel, which has the effect of creating a drug reservoir. Both drug and gel release from the in situ gel system operated under zero-order kinetics and showed a correlation of release of PTX with gel, indicating a predominating release mechanism of the erosion type. Dispersing liposomes into the gel replaced larger gel itself for achieving the same gel dissolution rate. Both the critical micelle temperature and the sol/gel temperature, detected by DSC thermal analysis, were shifted to lower temperatures by adding liposomes. The extent of the shifts depended on the amount of embedded liposomes. MTT assay and drug uptake studies showed that the treatment with PTX-loaded liposomal 18% Pluronic F127 yielded cytotoxicities, intercellular fluorescence intensity, and drug concentration in KB cells much higher than that of conventional liposome, while blank liposomal 18% Pluronic F127 gel was far less than the Cremophor EL ® vehicle and empty liposomes. Conclusions A thermosensitive hydrogel with embedded liposome is a promising carrier for hydrophobic anticancer agents, to be used in parenteral formulations for treating local cancers.

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Section: Correlation Between Gel Dissolution and Drug Releasesupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Thermoreversible Pluronic® F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies

Nie¹,

Hsiao²,

Pan³

et al. 2011

IJN

119

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“…It gels at room temperature and has mostly been used in controlled-release drug delivery (Barichello et al, 1999;Desai and Blanchard, 2000;El Kamel, 2002;Wenzel et al, 2002). However, it has previously been applied as part of a scaffold for rat neural progenitor cells in a model of neurotrauma (Vacanti et al, 2001) and as a construct to support the growth and differentiation of lung progenitor cells (Cortiella et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro

et al. 2008

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Stroke and spinal cord or brain injury often result in cavity formation. Stem cell transplantation in combination with tissue engineering has the potential to fill such a cavity and replace lost neurons. Several hydrogels containing unique features particularly suitable for the delicate nervous system were tested by determining whether these materials were compatible with fetal human neural stem cells (hNSCs) in terms of toxicity and ability to support stem cell differentiation in vitro. The hydrogels examined were pluronic F127 (PF127), Matrigel and PuraMatrix. We found that PF127, in a gelated (30%) form, was toxic to hNSCs, and Matrigel, in a gelated (1-50%) form, prevented hNSCs' normal capacity for neuronal differentiation. In contrast, PuraMatrix was the most optimal hydrogel for hNSCs, since it showed low toxicity when gelated (0.25%) and retained several crucial properties of hNSCs, including migration and neuronal differentiation. Further optimization and characterization of PuraMatrix is warranted to explore its full potential in assisting neural regeneration in vivo.

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“…Pluronic 1 F127 is a US Food and Drug Administration-approved nonionic surfactant/excipient used in inhalation, oral, and ophthalmic preparations with published safety data [3]. Injectable formulations have been investigated in animals [2,12,13]. Pluronic 1 F127 has high affinity for both water and MMA.…”

Section: Discussionmentioning

confidence: 99%

Surfactant-stabilized Emulsion Increases Gentamicin Elution From Bone Cement

Miller

McLaren

Leon

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Liquid antimicrobial use for antimicrobialloaded bone cement is limited because of decreased strength and small volume that can be loaded. Emulsifying the liquid antimicrobial into the monomer may address both issues. Questions/purposes We determined the effect of using a surfactant-stabilized emulsion on antimicrobial release, compressive strength, and porosity. Methods We made 144 standardized test cylinders from emulsified antimicrobial-loaded bone cement (three batches, 72 cylinders) and control antimicrobial-loaded bone cement made with antimicrobial powder (three batches, 72 cylinders). For each formulation, five specimens per batch (n = 15) were eluted in infinite sink conditions over 30 days for gentamicin delivery; five specimens per batch were axially compressed to failure after elution of 0, 1, and 30 days (n = 45); and two noneluted specimens and two gentamicin delivery specimens from each batch (n = 12) were examined under scanning electron microscopy for porosity. Antimicrobial release and compressive strength were compared across cement type and time using repeated-measures ANOVA. Results Emulsified antimicrobial-loaded bone cement released four times more antimicrobial than control. Compressive strength of emulsified antimicrobial-loaded bone cement was less than control before elution (58.1 versus 81.3 MPa) but did not decrease over time in elution. Compressive strength of control antimicrobial-loaded bone cement decreased over 30 days in elution (81.3 versus 73.9 MPa) but remained stronger than emulsified antimicrobial-loaded bone cement. Porosity was homogeneous, with pores ranging around 50 lm. Conclusions Emulsified antimicrobial-loaded bone cement has homogeneous porosity with increased drug release but a large loss of strength. Clinical Relevance Liquid antimicrobials are released from emulsified antimicrobial-loaded bone cement, but increased strength is needed before this method can be used for implant fixation.

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Absorption of insulin from Pluronic F-127 gels following subcutaneous administration in rats

Cited by 202 publications

References 31 publications

Thermoreversible Pluronic® F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies

Thermoreversible Pluronic® F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies

Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro

Surfactant-stabilized Emulsion Increases Gentamicin Elution From Bone Cement

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Absorption of insulin from Pluronic F-127 gels following subcutaneous administration in rats

Cited by 202 publications

References 31 publications

Thermoreversible Pluronic&reg; F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies

Thermoreversible Pluronic&reg; F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies

Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro

Surfactant-stabilized Emulsion Increases Gentamicin Elution From Bone Cement

Contact Info

Product

Resources

About

Thermoreversible Pluronic® F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies

Thermoreversible Pluronic® F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies