Absorption of iron from recombinant human lactoferrin in young US women

Lönnerdal, Bo; Bryant, Annika

doi:10.1093/ajcn/83.2.305

Cited by 52 publications

(38 citation statements)

References 19 publications

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“…These data seem to confirm the particular lactoferrin capacity to modulate iron homeostasis, and explain the maintenance of physiological levels of iron in the blood and suggest that the iron provided by lactoferrin is likely to be well used in human adults. Indeed, serum ferritin is inversely correlated with iron adsorption, and a http://theoncologist.alphamedpress.org/ beneficial effect of lactoferrin on iron acquisition in the gut is well documented [26]. Although the inflammation parameters assessed (i.e., CRP and ESR) did not show substantial differences at the end of treatment, their immediate decrease in the lactoferrin group should be highlighted.…”

Section: Discussionmentioning

confidence: 97%

Efficacy and Safety of Oral Lactoferrin Supplementation in Combination with rHuEPO-β for the Treatment of Anemia in Advanced Cancer Patients Undergoing Chemotherapy: Open-Label, Randomized Controlled Study

Macciò

Madeddu

Gramignano³

et al. 2010

The Oncologist

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Advanced-stage cancer patients often suffer from anemia that closely resembles the anemia of chronic inflammatory diseases characterized by specific changes in iron homeostasis and absorption. i.v. iron improves the efficacy of recombinant human erythropoietin (rHuEPO) in anemic cancer patients undergoing chemotherapy. We report the results of an open-label, randomized, prospective trial aimed at testing the efficacy and safety of treatment with oral lactoferrin versus i.v. iron, both combined with rHuEPO, for the treatment of anemia in a population of 148 advanced cancer patients undergoing chemotherapy. All patients received s.c. rHuEPO-␤, 30,000 UI once weekly for 12 weeks, and were randomly assigned to ferric gluconate (125 mg i.v. weekly) or lactoferrin (200 mg/day). Both arms showed a significant hemoglobin increase. No difference in the mean hemoglobin increase or the hematopoietic response, time to hematopoietic response, or mean change in serum iron, C-reactive protein, or erythrocyte sedimentation rate were observed between arms. In contrast, ferritin decreased in the lactoferrin arm whereas it increased in the i.v. iron arm. In conclusion, these results show similar efficacy for oral lactoferrin and for i.v. iron, combined with rHuEPO, for the treatment of anemia in advanced cancer patients undergoing chemotherapy. The Oncologist 2010;15:894 -902

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Section: Discussionmentioning

confidence: 97%

Efficacy and Safety of Oral Lactoferrin Supplementation in Combination with rHuEPO-β for the Treatment of Anemia in Advanced Cancer Patients Undergoing Chemotherapy: Open-Label, Randomized Controlled Study

Macciò

Madeddu

Gramignano³

et al. 2010

The Oncologist

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“…54 Even in the developed gut, iron is absorbed equally efficiently in the form of either lactotransferrin or a sulphate salt, 55 thus, there is little justification for the overcompensation in iron supplementation other than for prevention of anaemia in at-risk infants, such as those who are preterm. 20 Furthermore, high iron supplementation in rats with simulated iron deficiency anaemia failed to prevent neurochemical deficits.…”

Section: Box 2 | Evolution Of Iron Regulatory Mechanismsmentioning

confidence: 99%

Is early-life iron exposure critical in neurodegeneration?

et al. 2015

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The effects of iron deficiency are well documented, but relatively little is known about the long-term implications of iron overload during development. High levels of redox-active iron in the brain have been associated with neurodegenerative disorders, most notably Parkinson disease, yet a gradual increase in brain iron seems to be a feature of normal ageing. Increased brain iron levels might result from intake of infant formula that is excessively fortified with iron, thereby altering the trajectory of brain iron uptake and amplifying the risk of iron-associated neurodegeneration in later life. In this Perspectives article, we discuss the potential long-term implications of excessive iron intake in early life, propose the analysis of iron deposits in teeth as a method for retrospective determination of iron exposure during critical developmental windows, and call for evidence-based optimization of the chemical composition of infant dietary supplements.

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“…Lactoferrin (LF) is an 80 kDa, single subunit N-glycoprotein with multiple functions and involved in many processes, such as activation of the immune system [1][2][3][4] and inflammatory regulation [5][6][7], but also iron absorption in the intestinal tract [8], or in prevention of reactive oxygen species formation [9], and growth promotion in certain tissues [10,11]. Furthermore, LF has been shown to have inhibitory effects on tumourigenesis [12,13] and anti-metastatic activity [14][15][16].…”

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confidence: 99%

Use of Wisteria floribunda agglutinin affinity chromatography in the structural analysis of the bovine lactoferrin N-linked glycosylation

Leeuwen

Schoemaker

Timmer

et al. 2012

Biochimica et Biophysica Acta (BBA) - General Subjects

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Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Background: Over the years, the N-glycosylation of both human and bovine lactoferrin (LF) has been studied extensively, however not all aspects have been studied in as much detail. Typically, the bovine LF complex-type N-glycans include certain epitopes, not found in human LF N-glycans, i.e. Gal(α1-3)Gal(β1-4)GlcNAc (αGal), GalNAc(β1-4) GlcNAc (LacdiNAc), and N-glycolylneuraminic acid (Neu5Gc). The combined presence of complex-type N-glycans, with αGal, LacdiNAc, LacNAc [Gal(β1-4)GlcNAc], Neu5Ac (N-acetylneuraminic acid), and Neu5Gc epitopes, and oligomannose-type N-glycans complicates the high-throughput analysis of such N-glycoprofiles highly. Methods: For the structural analysis of enzymatically released N-glycan pools, containing both LacNAc and LacdiNAc epitopes, a prefractionation protocol based on Wisteria floribunda agglutinin affinity chromatography was developed. The sub pools were analysed by MALDI-TOF-MS and HPLC-FD profiling, including sequential exoglycosidase treatments.Results: This protocol separates the N-glycan pool into three sub pools, with (1) free of LacdiNAc epitopes, (2) containing LacdiNAc epitopes, partially shielded by sialic acid, and (3) containing LacdiNAc epitopes, without shielding by sialic acid. Structural analysis by MALDI-TOF-MS and HPLC-FD showed a complex pattern of oligomannose-, hybrid-, and complex-type di-antennary structures, both with, and without LacdiNAc, αGal and sialic acid. Conclusions: Applying the approach to bovine LF has led to a more detailed N-glycome pattern, including LacdiNAc, αGal, and Neu5Gc epitopes, than was shown in previous studies. General significance: Bovine milk proteins contain glycosylation patterns that are absent in human milk proteins; particularly, the LacdiNAc epitope is abundant. Analysis of bovine milk serum proteins is therefore excessively complicated. The presented sub fractionation protocol allows a thorough analysis of the full scope of bovine milk protein glycosylation. This article is part of a Special Issue entitled Glycoproteomics.

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Absorption of iron from recombinant human lactoferrin in young US women

Abstract: Iron is equally well absorbed from lactoferrin (whether heat-treated or untreated) and ferrous sulfate. Thus, iron provided by dietary lactoferrin is likely to be well utilized in human adults.

Cited by 52 publications

References 19 publications

Efficacy and Safety of Oral Lactoferrin Supplementation in Combination with rHuEPO-β for the Treatment of Anemia in Advanced Cancer Patients Undergoing Chemotherapy: Open-Label, Randomized Controlled Study

Efficacy and Safety of Oral Lactoferrin Supplementation in Combination with rHuEPO-β for the Treatment of Anemia in Advanced Cancer Patients Undergoing Chemotherapy: Open-Label, Randomized Controlled Study

Is early-life iron exposure critical in neurodegeneration?

Use of Wisteria floribunda agglutinin affinity chromatography in the structural analysis of the bovine lactoferrin N-linked glycosylation

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