Absorption spectroscopy in microfluidic flow cells using a metal clad leaky waveguide device with a porous gel waveguide layer

Bastani, Behnam; Goddard, N J; Grieve, Bruce

doi:10.1039/c2an35898k

Cited by 23 publications

(26 citation statements)

References 60 publications

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“…depth) with ease, and fabricating these devices without requiring specialised equipment (e.g. sputtering of gold for SPR devices and e-beam evaporation to deposit titanium in case of metal-clad leaky waveguide, MCLW) [19].…”

Section: Introductionmentioning

confidence: 99%

Optical waveguide for common path simultaneous refractive index and broadband absorption measurements in small volumes

Goddard

2016

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

Optical waveguide for common path simultaneous refractive index and broadband absorption measurements in small volumes

Goddard

2016

Sensors and Actuators B: Chemical

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“…A second advantage of using a low index waveguide is the ease of coupling light in and out of these waveguides using a prism, and is a result of their characteristic partial confinement of light. Low index waveguides have been variously termed Leaky Waveguides (LWs) [8,[17][18][19][20][21], Light Condensers (LCs) [22] or Hydrogel Optical Waveguides (HOGs) [23][24][25], but will be referred to as LWs hereafter.…”

Section: Methods Basic Principle Benefits Limitationsmentioning

confidence: 99%

A proof-of-principle study for performing enzyme bioassays using substrates immobilized in a leaky optical waveguide

Goddard

2017

Sensors and Actuators B: Chemical

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“…Combining Raman with microfluidics allows for the accurate monitoring, analysis, and detection of a wide range of samples in micro-environments. So far, microfluidic platforms integrated with SERS for biochemical analysis have been extensively reported [16,17,18,19,20,21,22,23,24]. One of the major challenges of combining microfluidics and Raman spectroscopy is the strong background from substrates.…”

Section: Introductionmentioning

confidence: 99%

Use of Surface-Enhanced Raman Scattering (SERS) Probes to Detect Fatty Acid Receptor Activity in a Microfluidic Device

Zhang

Xiao

et al. 2019

Sensors

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In this study, 4-mercaptobenzoic acid (MBA)-Au nanorods conjugated with a GPR120 antibody were developed as a highly sensitive surface-enhanced Raman spectroscopy (SERS) probe, and were applied to detect the interaction of fatty acids (FA) and their cognate receptor, GPR120, on the surface of human embryonic kidney cells (HEK293-GPRR120) cultured in a polydimethylsiloxane (PDMS) microfluidic device. Importantly, the two dominant characteristic SERS peaks of the Raman reporter molecule MBA, 1078 cm−1 and 1581 cm−1, do not overlap with the main Raman peaks from the PDMS substrate when the appropriate spectral scanning range is selected, which effectively avoided the interference from the PDMS background signals. The proposed microfluidic device consisted of two parts, that is, the concentration gradient generator (CGG) and the cell culture well array. The CGG part was fabricated to deliver five concentrations of FA simultaneously. A high aspect ratio well structure was designed to address the problem of HEK cells vulnerable to shear flow. The results showed a positive correlation between the SERS peak intensity and the FA concentrations. This work, for the first time, achieved the simultaneous monitoring of the Raman spectra of cells and the responses of the receptor in the cells upon the addition of fatty acid. The development of this method also provides a platform for the monitoring of cell membrane receptors on single-cell analysis using SERS in a PDMS-based microfluidic device.

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Absorption spectroscopy in microfluidic flow cells using a metal clad leaky waveguide device with a porous gel waveguide layer

Cited by 23 publications

References 60 publications

Optical waveguide for common path simultaneous refractive index and broadband absorption measurements in small volumes

Optical waveguide for common path simultaneous refractive index and broadband absorption measurements in small volumes

A proof-of-principle study for performing enzyme bioassays using substrates immobilized in a leaky optical waveguide

Use of Surface-Enhanced Raman Scattering (SERS) Probes to Detect Fatty Acid Receptor Activity in a Microfluidic Device

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