Abstract 1500: A novel nutrient mixture exhibits antitumor activity in human fibrosarcoma cell line HT-1080

Abstract

Introduction: Fibrosarcoma, an aggressive and highly metastatic cancer of connective tissue, is generally associated with poor prognosis. Cancer mortality usually results from tumor invasion of local tissue and metastasis to vital organs. We investigated the effect of a specific nutrient mixture (PB) of quercetin, cruciferex, curcumin, green tea extract and resveratrol on human fibrosarcoma cell line HT-1080 for viability, MMP expression, invasion, apoptosis and morphology. Material and Methods: Human fibrosarcoma cell line HT-1080 (ATCC) was cultured in DEM media, supplemented with 10% FBS and antibiotics in 24-well tissue plates. At near confluence, cells were treated with PB at 0, 10, 25, 50, 75 and 100 mcg/mL, in triplicate at each dose. Cells were also treated with PMA (100 ng/mL) for MMP-9 induction. Cell proliferation was assayed by MTT assay, MMP by zymography, invasion through Matrigel, apoptosis using green caspase detection, and morphology by H&E staining. Results: PB inhibited proliferation of human fibrosarcoma cells HT-1080 by 50% at 10 mcg/mL, 60% at 25 mcg/mL and 80% at 50-100 mcg/mL, and inhibited the secretion of both MMP-2 and MMP-9 in a dose-dependent manner, with total inhibition at 50 mcg/mL concentration. Invasion through Matrigel was inhibited 100% at 25 mcg/mL PB. PB also induced apoptosis in a dose- dependent fashion. H&E staining showed slight morphological changes at higher concentrations. Conclusion: These results suggest that PB is a potential therapeutic agent for fibrosarcoma, with potent anti-metastatic activity, because it inhibited fibrosarcoma cell proliferation, MMP-2 and -9 expression, invasion through Matrigel and apoptosis, important parameters for cancer prevention. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1500. doi:10.1158/1538-7445.AM2011-1500

The effect of chlorophyllin, a glutathione transferase P1‐1 inhibitor, on triple-negative breast cancer invasion and metastasis in vivo/in vitro

“…Although both docetaxel and chlorophyllin could inhibit cell migration alone, their combined treatment had a substantially stronger effect. There was no available study in literature investigating the effects of combined use of chlorophyllin with anticancer drugs on cell migration, however, these results were in line with other studies reporting that certain antioxidants and/or phytochemicals administration, such as chlorophyllin, reduced the cell migration in aggressive cancer cell lines (Lirdprapamongkol, Sakurai et al 2005, Roomi, Bhanap et al 2018, Dehghani, Kooshafar et al 2019). In a study on HT-1080 brosarcoma cells, it was shown that chlorophyllin dose-dependently reduced the cell migration, and this effect was highest at 50 µM chlorophyllin administration (Roomi, Bhanap et al 2018).…”

Anticancer Efficacy of Polyphenols and Their Combinations

“…The inhibitory effects of PB were also observed in fibrosarcoma, which is an aggressive and highly metastatic cancer of connective tissue, and in melanoma, which causes the majority of skin cancer-related deaths, secondary to metastasis. The in vitro studies used human fibrosarcoma cell line HT-1080 [ 96 ] and melanoma A2058 cells [ 97 ]. The study showed that PB inhibited cell proliferation of human fibrosarcoma cells by 60% at 25 μg/mL and 80% at 50 μg/mL, and inhibited both MMP-2 and -9 secretion in a dose-dependent manner, with total inhibition at 50 μg/mL [ 96 ].…”

“…The in vitro studies used human fibrosarcoma cell line HT-1080 [ 96 ] and melanoma A2058 cells [ 97 ]. The study showed that PB inhibited cell proliferation of human fibrosarcoma cells by 60% at 25 μg/mL and 80% at 50 μg/mL, and inhibited both MMP-2 and -9 secretion in a dose-dependent manner, with total inhibition at 50 μg/mL [ 96 ]. At PB concentration of 25 μg/mL, invasion of HT-1080 cells through Matrigel was totally blocked [ 96 ].…”

“…The study showed that PB inhibited cell proliferation of human fibrosarcoma cells by 60% at 25 μg/mL and 80% at 50 μg/mL, and inhibited both MMP-2 and -9 secretion in a dose-dependent manner, with total inhibition at 50 μg/mL [ 96 ]. At PB concentration of 25 μg/mL, invasion of HT-1080 cells through Matrigel was totally blocked [ 96 ]. Melanoma cells were more sensitive to PB, as their proliferation was inhibited by 80% at 25 ug/mL and MMP-2 and -9 secretion and Matrigel cell invasion blocked completely at 50 μg/mL, indicating strong anti-invasive properties of this polyphenol mixture [ 97 ].…”

Enhancement of Annexin V in response to combination of epigallocatechin gallate and quercetin as a potent arrest the cell cycle of colorectal cancer

“…The chemical composition of the PB contained. Previous studies have shown the anti-proliferative and anti-cancerous potential of PB in breast, sarcoma, skin and head and neck cancer cell lines (Alqarni et al, 2017;Niedzwiecki et al, 2016;Roomi et al, 2011Roomi et al, , 2015.…”