Abstract 56

A novel diagnostic biosensor for distinguishing immunoglobulin mutated and unmutated types of chronic lymphocytic leukemia

“…There are several methods to quantify ZAP-70 including: polymerase chain reaction (PCR), immunoblotting, immunohistochemistry, fluorescence in-situ hybridization (FISH) analysis and flow cytometry (Slack et al, 2007, Wang et al, 2012, Put et al, 2009, Vroblova et al, 2012, Matthews et al, 2004, Moreno and Montserrat, 2010. Use of flow cytometry for ZAP-70 detection seems to be advantageous as this technique enables us to assess the presence of ZAP-70 separately on CLL clone, T-cells and NK-cells.…”