Abundance and Activity of Methanotrophic Bacteria in Littoral and Profundal Sediments of Lake Constance (Germany)

Rahalkar, Monali C.; Deutzmann, Jörg S.; Schink, Bernhard; Bussmann, Ingeborg

doi:10.1128/aem.01350-08

Cited by 81 publications

(71 citation statements)

References 65 publications

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“…Hence, type I was most likely the dominant group of methanotrophic bacteria in all of these sediments. This finding is in agreement with other studies in sediments of freshwater habitats (Boschker et al 1998, Costello et al 2002, Eller et al 2005, Rahalkar et al 2009). Boschker et al (1998) showed the dominance of type I methanotrophs for the first time with C 13 -methane labeling experiments.…”

Section: Discussionsupporting

confidence: 83%

Comparative study on bacterial carbon sources in lake sediments: the role of methanotrophy

Steger¹,

Premke²,

Gudasz³

et al. 2015

Aquat. Microb. Ecol.

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Methane-derived carbon can be important in both benthic and pelagic food webs. Either generated in the anaerobic layers of the sediment or in the anaerobic hypolimnion of stratified eutrophic lakes, methane is an excellent carbon source for aerobic methanotrophic bacteria. The very negative methane δ 13 C-signal in the methanotrophic biomass provides an excellent opportunity to trace the use of methane-derived carbon in food webs. We studied carbon sources of benthic bacteria in a range of Swedish lakes with different inputs of terrestrial organic carbon and indigenous primary production. We analyzed the 13 C: 12 C ratios in phospholipid-derived fatty acids, which serve as biomarkers for specific groups of Bacteria. We demonstrate that methane is an important carbon source for sediment bacteria, not only for the methanotrophic community but also for the non-methanotrophic heterotrophic bacteria. This most likely indirect utilization of isotopically highly depleted methane masks the stable isotope signatures for terrestrial input and local primary production in the heterotrophic bacterial community.

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Section: Discussionsupporting

confidence: 83%

Comparative study on bacterial carbon sources in lake sediments: the role of methanotrophy

Steger¹,

Premke²,

Gudasz³

et al. 2015

Aquat. Microb. Ecol.

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“…This presentation of a direct and statistically significant linear relationship is the first to our knowledge. It agrees with other qualitative reports of positive correlations between methane oxidation rates and abundance of pmoA or MOB 16S rRNA genes determined using a variety of methods -quantitative PCR, FISH, or sequencing -for marine water column and lake sediments (Crespo-Medina et al, 2014;Deutzmann et al, 2011;Rahalkar et al, 2009;Steinle et al, 2015). Future application of marine-specific pmoA primers may further improve this correlation (Tavormina et al, 2008).…”

Section: Abundances Of Mob and Non-mob Methylotrophs Control The Methsupporting

confidence: 77%

Methane-oxidizing seawater microbial communities from an Arctic shelf

et al. 2018

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Abstract. Marine microbial communities can consume dissolved methane before it can escape to the atmosphere and contribute to global warming. Seawater over the shallow Arctic shelf is characterized by excess methane compared to atmospheric equilibrium. This methane originates in sediment, permafrost, and hydrate. Particularly high concentrations are found beneath sea ice. We studied the structure and methane oxidation potential of the microbial communities from seawater collected close to Utqiagvik, Alaska, in April 2016. The in situ methane concentrations were 16.3 ± 7.2 nmol L −1 , approximately 4.8 times oversaturated relative to atmospheric equilibrium. The group of methaneoxidizing bacteria (MOB) in the natural seawater and incubated seawater was > 97 % dominated by Methylococcales (γ -Proteobacteria). Incubations of seawater under a range of methane concentrations led to loss of diversity in the bacterial community. The abundance of MOB was low with maximal fractions of 2.5 % at 200 times elevated methane concentration, while sequence reads of non-MOB methylotrophs were 4 times more abundant than MOB in most incubations. The abundances of MOB as well as non-MOB methylotroph sequences correlated tightly with the rate constant (k ox ) for methane oxidation, indicating that non-MOB methylotrophs might be coupled to MOB and involved in community methane oxidation. In sea ice, where methane concentrations of 82 ± 35.8 nmol kg −1 were found, Methylobacterium (α-Proteobacteria) was the dominant MOB with a relative abundance of 80 %. Total MOB abundances were very low in sea ice, with maximal fractions found at the icesnow interface (0.1 %), while non-MOB methylotrophs were present in abundances similar to natural seawater communities. The dissimilarities in MOB taxa, methane concentrations, and stable isotope ratios between the sea ice and water column point toward different methane dynamics in the two environments.

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“…The finding that the maximum of methanotrophic activity in Lake Kinneret occurred where oxygen concentrations were below the detection limit is in agreement with recent results from littoral and profundal sites in Lake Constance (Rahalkar et al 2009). Similarly, in the Black Sea water column, aerobic methanotrophs and methane oxidation were recorded at water depths in which oxygen concentration was below the detection limit (Schubert et al 2006).…”

Section: Discussionsupporting

confidence: 81%

“…Similarly, in the Black Sea water column, aerobic methanotrophs and methane oxidation were recorded at water depths in which oxygen concentration was below the detection limit (Schubert et al 2006). It is still unclear whether methanotrophs are responsible for the methane oxidation under these conditions (Schubert et al 2006, Rahalkar et al 2009), since they require oxygen as an electron acceptor. It has been postulated that MOB can also be responsible for methane oxidation under anaerobic conditions (Rahalkar et al 2009), probably by using an alternative terminal electron acceptor like nitrate.…”

Section: Discussionmentioning

confidence: 99%

“…It is still unclear whether methanotrophs are responsible for the methane oxidation under these conditions (Schubert et al 2006, Rahalkar et al 2009), since they require oxygen as an electron acceptor. It has been postulated that MOB can also be responsible for methane oxidation under anaerobic conditions (Rahalkar et al 2009), probably by using an alternative terminal electron acceptor like nitrate. In the case of Lake Kinneret, it is still possible that internal seiche tilting of the chemocline, shear flow, and turbulence contribute to mixing and displacement of water layers within the metalimnion, allowing oxygen to reach the methane at the lower part of the chemocline.…”

Section: Discussionmentioning

confidence: 99%

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Methane- and ammonia-oxidizing bacteria at the chemocline of Lake Kinneret (Israel)

Junier¹,

Kim²,

Eckert³

et al. 2010

Aquat. Microb. Ecol.

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The vertical distribution of methane- and ammonia-oxidizing bacteria (MOB and AOB, respectively), and the physicochemical conditions in the chemocline of Lake Kinneret (Israel) were studied at a resolution of 10 cm from 16.2 to 17.7 m depth. Profiles of the chemical parameters indicated decreasing concentrations of methane (from 22.4 to 0.11 µmol l–1) and ammonia (from 14.2 to 8.4 µmol l–1) towards the water surface and in close proximity to the chemocline. The disappearance of methane coincided with methane oxidation that could be corroborated throughout this layer with highest rates at 17.4 to 17.6 m. Disappearance of ammonia could not be linked to ammonia oxidation exclusively. The genes pmoA and the homologous amoA (coding for subunit α of the methane and ammonia monooxygenase, respectively) were amplified by PCR. The products were analyzed by terminal restriction fragment length polymorphism (T-RFLP) and sequencing of clone libraries. The results demonstrated that different MOB and AOB communities are established along the concentration gradient within the narrow layer of the metalimnetic chemocline. Changes in the intensity of the T-RFLP peaks and the frequency of different groups of alpha- and gammaproteobacterial MOB, and betaproteobacterial AOB, coincided with the concentration gradients of methane, ammonia, nitrate, and oxygen in the chemocline. This suggests that different communities of MOB, and to a lesser extent AOB, contribute to the formation of chemical gradients of their particular substrates in the chemocline

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Abundance and Activity of Methanotrophic Bacteria in Littoral and Profundal Sediments of Lake Constance (Germany)

Cited by 81 publications

References 65 publications

Comparative study on bacterial carbon sources in lake sediments: the role of methanotrophy

Comparative study on bacterial carbon sources in lake sediments: the role of methanotrophy

Methane-oxidizing seawater microbial communities from an Arctic shelf

Methane- and ammonia-oxidizing bacteria at the chemocline of Lake Kinneret (Israel)

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