Vultures are currently among the most endangered species in the world. Their populations have significantly declined during the last decades. In West Africa, threats mainly include persecution for belief-based use (e.g., traditional medicine and succeeding in business), accidents, and degradation of natural habitats. In Senegal, knowledge of vulture populations is lacking; hence, we were interested in studying them as part of the Great Green Wall (GGW) project (officially known as the Grande Muraille Verte project). Initiated in 2009 by African heads of state, the GGW project involves reforestation designed to restore ecosystems in the Sahel that have been degraded by climate change and harmful human activity. It also helps to combat poverty among local populations by developing the natural potential of their environment. Over a 3-yr period, we searched for occupied nests, communal roost sites and frequent perches, and other signs of vulture presence within a 16-km radius of Koyli Alpha, a village situated within the GGW extension project area in northern Senegal. We documented 19 occupied vulture nests within this study area, 10 belonging to Rüppell’s Vultures (Gyps rueppelli), 7 belonging to White-backed Vultures (Gyps africanus), 1 belonging to Hooded Vultures (Necrosyrtes monachus), and 1 belonging to Lappet-faced Vultures (Torgos tracheliotos). Vultures nested primarily in Acacia raddiana and baobob (Adansonia digitata). Griffon Vultures (Gyps fulvus), Egyptian Vultures (Neophron percnopterus), and White-headed Vultures (Trigonoceps occipitalis) also were observed roosting and foraging in the area. The main threats to vultures in the study area are habitat fragmentation, destruction of natural habitats by anthropogenic activities, and poaching to obtain vulture parts for traditional practices for belief-based use. Our results concerning vulture species diversity and ecology reinforce the idea that the Koyli Alpha area should be designated as an Important Bird Area or a key biodiversity area. Strict application of the laws governing these protected areas would ensure better protection for these highly endangered species.