Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui, Japan

Kondo, Ryou; Osawa, Kyoko; Mochizuki, Lisa; Fujioka, Yukiyasu; Butani, Junki

doi:10.3800/pbr.1.165

Cited by 26 publications

(25 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This gas was then present in the deoxygenated layer, a few metres below the oxygenated zone. Kondo et al (2006) suggested that anaerobic sulfate-reducing bacteria were responsible for its formation. The ion concentration in the monimolimnion increased with depth (r=0.98, P<0.05), regardless of temperature (r=0.19, P>0.05).…”

Section: Microbiological Parametersmentioning

confidence: 99%

Relationships between physico-chemical and microbiological parameters in the monimolimnion of a forest meromictic lake

Górniak

Tandyrak²,

Parszuto³

et al. 2014

J Limnol

View full text Add to dashboard Cite

show abstract

Section: Microbiological Parametersmentioning

confidence: 99%

Relationships between physico-chemical and microbiological parameters in the monimolimnion of a forest meromictic lake

Górniak

Tandyrak²,

Parszuto³

et al. 2014

J Limnol

View full text Add to dashboard Cite

show abstract

“…We previously developed new PCR primers selective for the dsrA of most mesophilic SRB belonging to d-Proteobacteria and used a quantitative competitive PCR for sensitive detection and enumeration of SRB populations as an alternative to culturedependent methods (Kondo et al 2004). This primer set has been used widely for detecting and enumerating SRB from marine and limnic environments (Kawahara et al 2008, Kondo et al 2006, Kondo & Butani 2007, Leloup et al 2007, Neretin et al 2007, Schippers & Neretin 2006, Webster et al 2006. Schippers & Neretin (2006) and Leloup et al (2007) have already used quantitative real-time PCR (qPCR) using our primer set for enumerating SRB in marine sediments, but there is no report on the application for water samples.…”

mentioning

confidence: 99%

Rapid enumeration of sulphate-reducing bacteria from aquatic environments using real-time PCR

Kondo

Shigematsu

Butani

2008

Plankton Benthos Res

Self Cite

View full text Add to dashboard Cite

Abstract:We describe a rapid and simple enumeration method for sulphate-reducing bacteria (SRB) from aquatic environments using a quantitative real-time PCR (qPCR) in combination with a rapid DNA isolation method. Enumeration of SRB in the sediment and water samples was performed by quantifying the copy number of the dsrA gene coding for the a-subunit of the dissimilatory sulphite reductase using real-time PCR with the SYBR Green I assay. Using dsrA-specific primers, we demonstrated that quantification of SRB in known numbers of SRB assemblages can be achieved. We compared DNA isolation methods using commercial DNA extraction kits and a published technique. We found two commercial kits were of advantage for extraction of DNA from water or sediment samples, where a large number of samples could be processed at the same time. We showed this newly developed qPCR technique targeting dsrA is rapid, simple and reproducible for the quantification of SRB numbers in situ and is superior to the use of culture-dependent techniques.

show abstract

“…These organisms completely oxidize a diverse range of organic compounds, including acetate and other short chain fatty acids, to CO2. Similar sequences have also been recovered from other marine and freshwater environments 1,16,41) . Desulfosarcina has been reported to be numerically abundant in sediment of the German Wadden Sea 25) .…”

Section: Phylogenetic Analysesmentioning

confidence: 50%

Spatial Dynamics of Sulphate-reducing Bacterial Compositions in Sediment along a Salinity Gradient in a UK Estuary

et al. 2007

Self Cite

View full text Add to dashboard Cite

The diversity of sulphate-reducing bacteria (SRB) was investigated in sediments along environmental gradients in the River Colne estuary, Essex, UK. DNA samples were collected from four sites; marine-dominated (Alresford Creek), brackish (the Hythe), predominantly freshwater (East Hill Bridge) and freshwater (the Weir) between September 2001 and May 2002. SRB community composition was assessed by PCR amplification, cloning and sequencing of part of the α subunit of dissimilatory sulphite reductase (dsrA) using directly extracted sediment DNA. The majority of the dsrA sequences were associated with members of the Desulfobacteraceae family, the Desulfobulbaceae family and a deeply branched group in the dsrA tree with no cultured representatives. There was some evidence of a salinity-related distribution within both the Desulfobacteraceae and Desulfobulbaceae groups. Clones related to Desulfotomaculum of the xenologues Firmicutes and a phylogenetically distinct Colne group 3 were detected only at the freshwater East Hill Bridge and Weir sites. Conversely, clones related to an uncultured group (Colne group 1) were found only at the marine and brackish sites. A statistical analysis of composition revealed that dsrA sequences from the marine-dominated Alresford Creek and the brackish site at the Hythe were not significantly different from each other (P>0.05), but were significantly different from those of the freshwater-dominated East Hill Bridge and the Weir (P<0.05). The sequences from East Hill Bridge and the Weir were not significantly different from each other (P>0.05). The data presented show a complex distribution of SRB along the estuary with some evidence to support the idea that salinity and sulphate concentrations are an important factor in determining SRB community structure.Key words: dissimilatory sulphite reductase gene, environmental gradients, estuarine sediment, sulphate reduction Sulphate reduction is an important process involved in both the global carbon and sulphur cycles. This process can dominate anaerobic terminal mineralisation of organic matter in high-sulphate sediments, degrading up to 50% of all organic matter in coastal marine sediments 12) and plays a minor but still important role in low-sulphate sediments 26,38) . Rates of sulphate reduction vary in relation to temperature and electron donor and acceptor concentrations with the availability of sulphate an important factor in the control of the rate of sulphate reduction. Sulphate concentrations range from approximately 25 mM in full-strength seawater to <0.2 mM in freshwater 7) . Freshwater-adapted sulphatereducing bacteria (SRB) have a greater affinity for sulphate (K m =5-68 µM) than marine-adapted SRB (K m =200 µM) 8,37) , allowing them to continue sulphate reduction at much lower sulphate concentrations than marine SRB. Physiological differences between freshwater-and marine-adapted SRB may be reflected in differences in species or phylogenetic compositions of SRB communities.The Colne estuary is a small, muddy, macrotidal (3 to 5...

show abstract

Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui, Japan

Cited by 26 publications

References 48 publications

Relationships between physico-chemical and microbiological parameters in the monimolimnion of a forest meromictic lake

Relationships between physico-chemical and microbiological parameters in the monimolimnion of a forest meromictic lake

Rapid enumeration of sulphate-reducing bacteria from aquatic environments using real-time PCR

Spatial Dynamics of Sulphate-reducing Bacterial Compositions in Sediment along a Salinity Gradient in a UK Estuary

Contact Info

Product

Resources

About