Abundance and diversity of vectors (Diptera: Psychodidae) in an old transmission area of cutaneous leishmaniasis in the new world after Bolivia-Brazil gas pipeline construction

Gonçalves, André Luiz; Luz, Edna Dora Martins Newman; Castro, Edilene Alcântara de; Klisiowicz, Débora do Rocio; Gazda, Tatiana Louise; Melo, André Luiz de Almeida; Soccol, Vanete Thomaz

doi:10.18004/mem.iics/1812-9528/2019.017.02.16-023

Cited by 1 publication

(3 citation statements)

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“…The high genetic diversity verified herein cannot suggest an independence between the cycles of wild, peridomicile, and household transmission of ACL, because these ecotypes are very close to each other and lack the geographical barriers that could separate the populations according to ecotypes or strains (Saraiva et al, 2012;Gonçalves et al, 2019). Therefore, the high genetic variability shown for L. intermedia, combined with its high prevalence, supports L. intermedia as a vector of L. braziliensis in the Ribeira Valley.…”

Section: Discussionmentioning

confidence: 68%

“…Samples were subjected to molecular analysis by RAPD-PCR (Polymerase chain reaction), selected from 52 individuals (45 males and five females of L. intermedia s.l. and two females of L. intermedia s.s.) out of a total of 403 previously collected specimens (Gonçalves et al, 2019).…”

Section: Molecular/rapd Analysismentioning

confidence: 99%

“…Ribeira River Valley is a region heavily influenced by anthropogenic changes. The Phlebotominae sand fly populations have changed their profile in relation to previous research over recent decades, and Lutzomyia (Nyssomyia) intermedia (Lutz and Neiva, 1912) have been currently found in abundance and considered the vector of Leishmania in domestic, peridomestic and sylvatic environments (Forattini et al, 1976;Castro et al, 2005;Gonçalves et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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GENETIC DIVERSITY OF Lutzomyia (Nyssomyia) intermedia IN AN ENDEMIC AREA OF AMERICAN CUTANEOUS LEISHMANIASIS, STATE OF PARANÁ, BRAZIL

Gonçalves¹,

Castro

Luz

et al. 2021

Acta biol. Colomb.

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Lutzomyia intermedia (Diptera: Psychodidae) features as one of the main vectors that are involved in the transmission of American cutaneous leishmaniasis (ACL) in the Neotropical region. However, genetic studies involving this taxon are still incipient and important for understanding the level of variability of different populations, their role, and implications as vectors. The aim of this study was to determine the level of genetic diversity of L. intermedia present in the Ribeira River Valley, an area of ACL transmission in the state of Paraná, Brazil, through the Random amplified polymorphic DNA (RAPD). Two municipalities were chosen to collect sand flies: Cerro Azul (new transmission area of the ACL) and Adrianópolis (endemic area of the ACL). The insects were captured in the house, in the peridomicile and in the wild (forest). Two of the used markers made it possible to estimate the polymorphism of the studied populations, resulting in 40 genotypes, most of them from peridomicile. The dendrogram generated by the analysis with the primer A10 showed different degrees of similarity, suggesting that there may be gene flow in the studied populations. The Principal Coordinate Analysis (PCO) with the A2 primer, was useful in grouping L. intermedia according to its ecological and geographical origin. There was no distinction between the lineages composing the L. intermedia complex. The results of this study, with the record of great genotypic diversity in L. intermedia, may contribute to explain the maintenance of the life cycle of Leishmania braziliensis (Kinetoplastida: Trypanosomatidae) in the region.

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Section: Discussionmentioning

confidence: 68%

Section: Molecular/rapd Analysismentioning

confidence: 99%