Abundance and half-life of the distinct oat phytochrome A3 and A4 mRNAs

Higgs, David A.; Barnes, Linda; Colbert, James T.

doi:10.1007/bf00043659

Cited by 3 publications

(2 citation statements)

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“…One reason for this is that genes with unstable transcripts often encode proteins whose expression must be tightly controlled, such as those genes involved in growth and differentiation. Unstable transcripts include phytochrome [35,69] and certain auxin-induced transcripts in plants [28,45,52], and protooncogene and cytokine transcripts in mammalian cells [33,66]. The rapid decay of unstable transcripts is considered to be an active process for two main reasons.…”

Section: Cis-and Trans-acting Determinants Of Mrna Stabilitymentioning

confidence: 99%

Control of mRNA stability in higher plants

Abler

Green

1996

Plant Mol Biol

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The degradation rates of different mRNAs in higher plants can vary over a broad range and are regulated by a variety of endogenous and exogenous stimuli. During the past several years, efforts to better understand the control of mRNA stability in plants have increased considerably and this has led to improved methodologies and important mechanistic insights. In this review, we highlight some of the most interesting examples of plant transcripts that are controlled at the level of mRNA decay and discuss what has been learned from their study. Experiments that implicate or demonstrate the involvement of particular cis- and trans-acting factors in mRNA decay pathways are a major focus, as are those experiments that have led to mechanistic models. Emphasis is also placed on studies that address the relationship between translation and mRNA stability. Our current knowledge indicates that some of the determinants and pathways for mRNA decay may differ in plants compared to other eukaryotes, whereas others appear to be similar. This knowledge, coupled with the availability of biochemical, molecular and genetic approaches to elucidate plant mRNA decay mechanisms, should continue to lead to findings of novel and general significance.

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Section: Cis-and Trans-acting Determinants Of Mrna Stabilitymentioning

confidence: 99%

Control of mRNA stability in higher plants

Abler

Green

1996

Plant Mol Biol

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“…Unstable transcripts are particularly interesting because they often correspond to genes that must be controlled rapidly or stringently, such as those involved in regulating cell growth and differentiation. Transcripts that fall into this category include phytochrome mRNA (Higgs et al, 1995) and several auxin-induced transcripts in plants, mating-type transcripts in yeast (Peltz and Jacobson, 1992), and several proto oncogene transcripts in mammalian cells (Greenberg and Belasco, 1993).…”

Section: Introductionmentioning

confidence: 99%

New Molecular Phenotypes in the dst Mutants of Arabidopsis Revealed by DNA Microarray Analysis

et al. 2001

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In this study, DNA microarray analysis was used to expand our understanding of the dst1 mutant of Arabidopsis. The dst (downstream) mutants were isolated originally as specifically increasing the steady state level and the half-life of DST-containing transcripts. As such, txhey offer a unique opportunity to study rapid sequence-specific mRNA decay pathways in eukaryotes. These mutants show a threefold to fourfold increase in mRNA abundance for two transgenes and an endogenous gene, all containing DST elements, when examined by RNA gel blot analysis; however, they show no visible aberrant phenotype. Here, we use DNA microarrays to identify genes with altered expression levels in dst1 compared with the parental plants. In addition to verifying the increase in the transgene mRNA levels, which were used to isolate these mutants, we were able to identify new genes with altered mRNA abundance in dst1 . RNA gel blot analysis confirmed the microarray data for all genes tested and also was used to catalog the first molecular differences in gene expression between the dst1 and dst2 mutants. These differences revealed previously unknown molecular phenotypes for the dst mutants that will be helpful in future analyses. Cluster analysis of genes altered in dst1 revealed new coexpression patterns that prompt new hypotheses regarding the nature of the dst1 mutation and a possible role of the DST-mediated mRNA decay pathway in plants. Taylor, C.B., and Green, P.J. (1991). Genes with homology to fungal and S-gene RNases are expressed in Arabidopsis thaliana. Plant Physiol. 96, 980-984. Taylor, C.B., and Green, P.J. (1995). Identification and characterization of genes with unstable transcripts (GUTs) in tobacco. Plant Mol. Biol. 28, 27-38. Taylor, C.B., Bariola, P.A., DelCardayré, S.B., Raines, R.T., and Green, P.J. (1993). RNS2: A senescence-associated RNase of Arabidopsis that diverged from the S-RNases before speciation. Proc. Natl. Acad. Sci. USA 90, 5118-5122. Tucker, M., Valencia-Sanchez, M.A., Staples, R.R., Chen, J., Denis, C.L., and Parker, R. (2001). The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae. Cell 104, 377-386.Vakalopoulou, E., Schaack, J., and Shenk, T. (1991). A 32-kilodalton protein binds to AU-rich domains in the 3Ј untranslated regions of rapidly degraded mRNAs.

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Control of mRNA stability in higher plants

Abler

Green

1996

Post-Transcriptional Control of Gene Expression in Plants

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Abundance and half-life of the distinct oat phytochrome A3 and A4 mRNAs

Cited by 3 publications

References 42 publications

Control of mRNA stability in higher plants

Control of mRNA stability in higher plants

New Molecular Phenotypes in the dst Mutants of Arabidopsis Revealed by DNA Microarray Analysis

Control of mRNA stability in higher plants

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