Abundance and removal of antibiotic resistance genes (ARGs) in the rearing environments of intensive shrimp aquaculture in South China

Wang, Linglong; Su, Haijun; Hu, Xiaojuan; Xu, Yu; Xu, Wujie; Huang, Xiaoshuai; Li, Zhuojia; Cao, Yucheng; Wang, Guoliang

doi:10.1080/03601234.2018.1550310

Cited by 27 publications

(5 citation statements)

References 44 publications

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“…Sul1 has been shown to be a potential indicator for assessing contamination by antibiotic resistance genes (ARGs) in aquaculture environments ( Su et al, 2017 ). Recently Wang et al (2019) reported predominance of sul1 and cmlA among various antibiotic resistance genes (ARGs) in the rearing environments of intensive shrimp farms in South China.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Profiles and Molecular Characteristics of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Isolated From Shrimp Aquaculture Farms in Kerala, India

et al. 2021

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This study was undertaken to evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae in selected shrimp aquaculture farms (n = 37) in Kerala, South India and to characterize the isolates using molecular tools. Overall, a low prevalence of ESBL-producers was found in the farms, most likely due to the reduced antibiotic usage in the shrimp farming sector. Out of the 261 samples (77 shrimp and 92 each of water and sediment), 14 (5.4%) tested positive for ESBL-E. coli or ESBL-K. pneumoniae. A total of 32 ESBL-E. coli and 15 ESBL- K. pneumoniae were recovered from these samples. All ESBL isolates were cefotaxime-resistant with minimal inhibitory concentration (MIC) ≥32 μg/ml. Of all isolates, 9 (28.1%) E. coli and 13 (86.7%) K. pneumoniae showed simultaneous resistance to tetracycline, ciprofloxacin, and trimethoprim-sulfamethoxazole. PCR analysis identified CTX-M group 1 (blaCTX–M–15) as the predominant ESBL genotype in both E. coli (23, 71.9%) and K. pneumoniae (15, 100%). Other beta-lactamase genes detected were as follows: blaTEM and blaSHV (11 K. pneumoniae), blaCTX–M group 9 (9 E. coli), and blaCMY–2 (2 E. coli). Further screening for AMR genes identified tetA and tetB (13, 40.6%), sul1 (11, 34.4%), sul2 (9, 28.1%), catA and cmlA (11, 34.4%), qepA and aac(6′)-Ib-cr (9, 28.1%) and strAB and aadA1 (2, 6.3%) in E. coli, and qnrB (13, 86.7%), qnrS (3, 20%), oqxB (13, 86.7%), tetA (13, 86.7%), and sul2 (13, 86.7%) in K. pneumoniae isolates. Phylogenetic groups identified among E. coli isolates included B1 (4, 12.5%), B2 (6, 18.8%), C (10, 31.3%), D (3, 9.4%), and E (9, 28.1%). PCR-based replicon typing (PBRT) showed the predominance of IncFIA and IncFIB plasmids in E. coli; however, in K. pneumoniae, the major replicon type detected was IncHI1. Invariably, all isolates of K. pneumoniae harbored virulence-associated genes viz., iutA, entB, and mrkD. Epidemiological typing by pulsed-field gel electrophoresis (PFGE) revealed that E. coli isolates recovered from different farms were genetically unrelated, whereas isolates of K. pneumoniae showed considerable genetic relatedness. In conclusion, our findings provide evidence that shrimp aquaculture environments can act as reservoirs of multi-drug resistant E. coli and K. pneumoniae.

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Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Profiles and Molecular Characteristics of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Isolated From Shrimp Aquaculture Farms in Kerala, India

et al. 2021

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“…Water is considered as an important channel and carrier of ARB and ARGs in the environment [ 67 , 68 ] and led to aquatic environments becoming persistent ARG reservoirs [ 9 ]. Many studies that have been conducted in aquatic environments suggest that abiotic factors, for example, pH [ 69 ], temperature [ 70 , 71 ], salinity [ 61 , 68 , 72 ], organic carbon [ 4 ], heavy metal contents on the co-selection of antibiotic resistance [ 73 , 74 ], antibiotic concentration [ 75 , 76 ], nutrients such as P and N [ 54 ], dissolved oxygen [ 54 ] and climatic factors such as rainfall [ 77 ] and seasonality [ 19 ] can affect the abundance of ARGs along with microbial communities in the environment.…”

Section: Abiotic Factors Influence Spread Of Args In the Environmementioning

confidence: 99%

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Sivalingam

Poté

Prabakar³

2020

Pathogens

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Over the past decades, the rising antibiotic resistance bacteria (ARB) are continuing to emerge as a global threat due to potential public health risk. Rapidly evolving antibiotic resistance and its persistence in the environment, have underpinned the need for more studies to identify the possible sources and limit the spread. In this context, not commonly studied and a neglected genetic material called extracellular DNA (eDNA) is gaining increased attention as it can be one of the significant drivers for transmission of extracellular ARGS (eARGs) via horizontal gene transfer (HGT) to competent environmental bacteria and diverse sources of antibiotic-resistance genes (ARGs) in the environment. Consequently, this review highlights the studies that address the environmental occurrence of eDNA and encoding eARGs and its impact on the environmental resistome. In this review, we also brief the recent dedicated technological advancements that are accelerating extraction of eDNA and the efficiency of treatment technologies in reducing eDNA that focuses on environmental antibiotic resistance and potential ecological health risk.

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“…Concerns have been raised about the interactions of new pollutants and antibiotic-resistance genes in water [52]. Furthermore, researchers were interested in the relationship between water environmental variables (chemical oxygen demand, total organic carbon, dissolved organic carbon, suspended solids, and total phosphorus) and ARGs [53], as well as the promotion of bacterial resistance by wastewater discharge from aquaculture systems [54,55].…”

Section: Development Of Antimicrobial Resistance In Global Aquaculturementioning

confidence: 99%

Trends in and Future Research Direction of Antimicrobial Resistance in Global Aquaculture Systems: A Review

Xiao,

Wang,

Wang

et al. 2023

Sustainability

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The accelerated development of antibiotic resistance genes (ARGs) and antimicrobial resistance (AMR) in aquaculture environments due to the overuse of antibiotics is a global concern. To systematically understand the research trends in and key concepts of ARGs and AMR in aquaculture systems, this study employed the bibliometrix R-package to conduct a bibliometric analysis of the publication characteristics of ARGs and AMR in aquaculture systems from the Web of Science, published from 2000 to 2021. The results revealed that China has produced the most papers. China and the northern hemisphere countries work closely together. Collaboration and multidisciplinary research helped to better understand the impact of AMR in aquaculture on food security and human health. Antibiotic-resistant bacteria and ARGs in aquaculture, as well as the relationship between water environmental variables, antibiotic residuals, and ARGs, are the current research focus. One of the future directions is to establish a conclusive link among water environmental variables, antibiotics, and ARGs. Another future direction is the development of new economical and environmentally friendly technologies to treat AMR in aquaculture wastewater. Collectively, our findings investigate the development directions of AMR research in global aquaculture systems and provide future perspectives.

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Abundance and removal of antibiotic resistance genes (ARGs) in the rearing environments of intensive shrimp aquaculture in South China

Cited by 27 publications

References 44 publications

Antibiotic Resistance Profiles and Molecular Characteristics of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Isolated From Shrimp Aquaculture Farms in Kerala, India

Antibiotic Resistance Profiles and Molecular Characteristics of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Isolated From Shrimp Aquaculture Farms in Kerala, India

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Trends in and Future Research Direction of Antimicrobial Resistance in Global Aquaculture Systems: A Review

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