Abundance of adiponectin mRNA transcript in the buffalo corpus luteum during the estrous cycle and effects on progesterone secretion in vitro

Expression and localization of adiponectin and its receptors in ovarian follicles during different stages of development and the modulatory effect of adiponectin on steroid production in water buffalo

“…In another study, the system was demonstrated in GC, theca cells and oocytes of the cattle ovary (Maillard et al, ). Recently, we have demonstrated the adiponectin expression in different stages of CL functionality in buffalo (Gupta, Thakre, Bahiram, et al, ). The differences in the expression pattern of adiponectin system across the species, suggests a species‐specific mode of action of the adiponectin system on follicular development and functions.…”

“…The presence of adiponectin and its receptors messenger RNA (mRNA) and protein has been shown in different cell types of bovine ovary including theca cells and GCs, cumulus‐oocyte complex and CL, furthermore, it is also documented that the adiponectin modulates steroid synthesis and the effects are varied in different cells of bovine ovary (Maillard et al, ; Tabandeh et al, ). Recently, we have shown the presence of adiponectin, AdipoR1 and AdipoR2 in buffalo CL and the effect of adiponectin on P 4 secretion from luteal cells (Gupta, Thakre, Bahiram, et al, ). However, till date, no data are available on the gene and protein expression of the adiponectin system in ovarian follicles buffalo ( Bubalus bubalis ) and its effect on steroidogenesis.…”

Adiponectin Influences FGF2 in the Developing Porcine Corpus Luteum

“…The dispersed lutea cells were seeded at 3 × 10 6 cells/culture well (24-well polystyrene plate, Sigma-Aldrich) in attachment serum media (1 mL/well; DMEM supplemented with 10% (v/v) heat-inactivated charcoal stripped fetal bovine serum (CS-FBS; Avantor/VWR) and 5000 µg/mL penicillin/streptomycin (Sigma-Aldrich)) and incubated for 24 h in a humidified atmosphere of 5% CO 2 in air at 37 • C to equilibrate the cells to the culture condition, allow aggregation, and promote adherence. Following the 24 h incubation, attachment serum media were aspirated and replaced with culture media (1 mL/well; DMEM, 0.1% (w/v) BSA, 2.0% (v/v) CS-FBS, 0.5 mM ascorbic acid, and 5000 µg/mL penicillin/streptomycin) containing recombinant porcine adiponectin (RD572023100, BioVender, Candler NC) at 1 µg, 3 µg, 6 µg (n = 3 wells/dose), or without adiponectin, 0 µg (control; n = 3 wells), for 24 h in a humidified atmosphere of 5% CO 2 in air at 37 • C. The range in culture doses of adiponectin was based on reported follicular fluid concentrations of adiponectin [36], plasma concentrations [25], and adiponectin-cultured endothelial and ovarian cells [16,26]. The inclusion of CS-FBS in the culture media provides an environment that enhances cell viability and supports angiogenic-related processes [37].…”

“…Furthermore, AdipoR2 mediates cellular action through PPARα activation [21] and the MAPK cascade [22], which are mechanisms that regulate Ang1 and FGF2 gene expression, respectively [23,24]. Both receptor isoforms have been identified in the porcine and bovine CL throughout the luteal phase [25,26], which implies a functional role for adiponectin in luteal tissue processes. In support, adiponectin has been reported to decrease luteal progesterone secretion [19,25], and microarray analysis of adiponectintreated mid-stage luteal cells revealed changes in pro-angiogenic factors [27].…”

Expression Profile of Orexin System in Corpus Luteum of Buffalo during Estrous Cycle

“…Primers of PPO, OX1R and OX2R, were designed using the Fast PCR (Version:6.2.73) software. Published primers were used for beta-actin (ACTB) and ribosomal protein 15 (RPL15) (Gupta et al, 2019). Details of the primers used, primer efficiencies and annealing temperature are provided in Table 1.…”