Abundance of estrogen receptors involved in non-canonical signaling in the dog testis

Gałuszka, Anna; Pawlicki, Piotr; Pardyak, Laura; Chmurska-Gąsowska, Maria; Pietsch-Fulbiszewska, Agnieszka; Duliban, Michał; Turek, Wiktor; Dubniewicz, Klaudia; Ramisz, Grzegorz; Kotula–Balak, Małgorzata

doi:10.1016/j.anireprosci.2021.106888

Cited by 5 publications

(6 citation statements)

References 61 publications

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“…The presence of GPER in germ cells (spermatocytes and/or round spermatids) of the normal testis has previously also been confirmed in human testis [ 27 , 44 ], which could suggest its interesting role in spermatogenesis. However, our findings disagree with results from equine [ 23 ] and canine testis [ 31 ], which showed GPER-IR exclusively in Leydig cells. This discrepancy could be related to different factors, including the diverse sensitivity of the employed antibody and/or fixation methods.…”

Section: Discussioncontrasting

confidence: 99%

“…Several studies have demonstrated the involvement of GPER in the tumorigenesis process, as well as its high over-expression in estrogen-dependent tumors in both human [49] and canine testis [31]. In fact, a promoting effect of GPER on cancer progression [22,49] has been proposed, albeit the tumor type could be a factor implicated into this role.…”

Section: Discussionmentioning

confidence: 99%

“…Studies in recent decades have elucidated the roles of estrogen in the physiology of the male reproductive tract, including spermatogenesis regulation and testicular steroidogenesis [ 14 ]. GPER mediates predominantly rapid non-genomic signaling of estrogens, and its presence in the testis has been observed in different animal species including horses [ 23 ], dogs [ 31 ], mice [ 43 ], and humans [ 27 , 44 ]. In particular, our results are in accordance with these studies for the localization of GPER and ERs in the Leydig cells, suggesting a role in the modulation of testicular steroidogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…In dogs, as in humans, the testis being located in abdominal cavity can result in developmental neoplasia (especially Sertoli cell tumor and seminoma) and torsion [ 2 ]. Several studies have demonstrated the involvement of GPER in the tumorigenesis process, as well as its high over-expression in estrogen-dependent tumors in both human [ 49 ] and canine testis [ 31 ]. In fact, a promoting effect of GPER on cancer progression [ 22 , 49 ] has been proposed, albeit the tumor type could be a factor implicated into this role.…”

Section: Discussionmentioning

confidence: 99%

“…GPER expression was investigated in the testis of the horse [ 23 ], rat [ 24 , 25 , 26 ] and human [ 27 ], while in the epididymis, its expression was reported in pigs [ 28 ], humans [ 29 ] and rats [ 30 ]. On the contrary, in dogs, limited studies have shown its presence in testes with and without tumors [ 31 ], and no data are available for dog epididymis. On the other hand, the pattern of classic estrogen receptors’ protein abundance was studied in the male reproductive tracts of some animal species including dogs [ 32 , 33 , 34 ], humans [ 35 ], and rodents [ 26 , 36 ], evidencing some differences in their distribution.…”

Section: Introductionmentioning

confidence: 99%

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G Protein-Coupled Estrogen Receptor (GPER) and ERs Are Modulated in the Testis–Epididymal Complex in the Normal and Cryptorchid Dog

Liguori,

Tafuri,

Pelagalli

et al. 2024

Veterinary Sciences

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There is growing evidence by the literature that the unbalance between androgens and estrogens is a relevant condition associated with a common canine reproductive disorder known as cryptorchidism. The role of estrogens in regulating testicular cell function and reproductive events is supposedly due to the wide expression of two nuclear estrogen receptors (ERs), ER-alpha and ER-beta and a trans-membrane G protein-coupled estrogen receptor (GPER) in the testis. In this study, immunohistochemistry, Western blotting and qRT-PCR were used to assess the distribution and expression of GPER in the testis–epididymal complex in the normal and cryptorchid dog. ER-alpha and ER-beta were also evaluated to better characterize the relative abundances of all three receptors. In addition, in these tissues, the expression level of two proteins as SOD1 and Nrf2 normally associated with oxidative stress was investigated to evaluate a possible relationship with ERs. Our data revealed changes in the distribution and expression of the GPER between the normal and cryptorchid dog. In particular, dogs affected by cryptorchidism showed an upregulation of GPER at level of the examined reproductive tract. Also considering the obtained result of a modulation of SOD1 and Nrf2 expression, we could hypothesize the involvement of GPER in the cryptorchid condition. Further studies are, however, necessary to characterize the role of GPER and its specific signaling mechanisms.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

G Protein-Coupled Estrogen Receptor (GPER) and ERs Are Modulated in the Testis–Epididymal Complex in the Normal and Cryptorchid Dog

Liguori,

Tafuri,

Pelagalli

et al. 2024

Veterinary Sciences

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Influence of deoxynivalenol and zearalenone on the immunohistochemical expression of oestrogen receptors and liver enzyme genes in vivo in prepubertal gilts

et al. 2023

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Deoxynivalenol (DON) and zearalenone (ZEN) are often detected in plant materials used to produce feed for pre-pubertal gilts. Daily exposure to small amounts of these mycotoxins causes subclinical conditions in pigs and affects various biological processes (e.g. mycotoxin biotransformation). The aim of this preclinical study was to evaluate the effect of low monotonic doses of DON and ZEN (12 µg/kg body weight—BW—and 40 µg/kg BW, respectively), administered alone or in combination to 36 prepubertal gilts for 42 days, on the degree of immunohistochemical expression of oestrogen receptors (ERs) in the liver and the mRNA expression of genes encoding selected liver enzymes during biotransformation processes. The level of expression of the analysed genes proves that the tested mycotoxins exhibit variable biological activity at different stages of biotransformation. The biological activity of low doses of mycotoxins determines their metabolic activity. Therefore, taking into account the impact of low doses of mycotoxins on energy-intensive processes and their endogenous metabolism, it seems that the observed situation may lead to the activation of adaptation mechanisms.

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Estrogen receptors alpha and beta expression in different canine cancer types with an emphasis on hematopoietic malignancies

Bugiel-Stabla,

Agnoli,

Pawlak

2024

Vet Res Commun

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Abundance of estrogen receptors involved in non-canonical signaling in the dog testis

Cited by 5 publications

References 61 publications

G Protein-Coupled Estrogen Receptor (GPER) and ERs Are Modulated in the Testis–Epididymal Complex in the Normal and Cryptorchid Dog

G Protein-Coupled Estrogen Receptor (GPER) and ERs Are Modulated in the Testis–Epididymal Complex in the Normal and Cryptorchid Dog

Influence of deoxynivalenol and zearalenone on the immunohistochemical expression of oestrogen receptors and liver enzyme genes in vivo in prepubertal gilts

Estrogen receptors alpha and beta expression in different canine cancer types with an emphasis on hematopoietic malignancies

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