Abundance of IFN-α and IFN-γ gene transcripts and absence of IL-2 transcripts in the blood of chickens vaccinated with live or inactivated NDV

Balenović, Mirta; Savić, Vladimir; Kabalin, Anamaria Ekert; Jurinović, Luka; Ragland, W.L.

doi:10.1556/avet.59.2011.1.13

Cited by 9 publications

(3 citation statements)

References 22 publications

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“…IFN-α and IFN-γ are involved in promoting innate immunity in response to viral infection. In addition, IFN-γ forms immune barrier to inhibit certain bacteria and protozoan infections, as well as activate macrophages ( 38 ). The expression levels of IFN-α and IFN-γ are negatively correlated with the intestinal inflammation and immunity.…”

Section: Discussionmentioning

confidence: 99%

Effects of Small Peptide Supplementation on Growth Performance, Intestinal Barrier of Laying Hens During the Brooding and Growing Periods

et al. 2022

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The growing period is a critical period for growth and development in laying hens. During this period, chicks grow rapidly, but are accompanied by unstable digestive function, incomplete organ development, and high mortality. Small peptide, a feed additive, which has been proved to promote intestinal development and immunity in poultry. In order to elucidate the effects of small peptides on growth performance, immunity, antioxidant capacity, and intestinal health of growing laying hens, a total of 900 Tianfu green shell laying hens (1-day-old) were randomly divided into 5 treatments with 6 replicates of 30 birds each in this 18-week trial. Dietary treatments included a corn-soybean meal-based diet supplemented with 0 g/kg, 1.5 g/kg, 3.0 g/kg, 4.5 g/kg and 6.0 g/kg small peptide, respectively. The results showed that the supplementation of small peptides significantly increased growth rate (P<0.05) in laying hens, as well as elevated the serum immunoglobulins (P<0.05) and antioxidant indices (P<0.05), however, it decreased inflammation parameters (P<0.05). The supplementation of small peptides enhanced the intestinal function by promoting gut development (P<0.05) and improving gut integrity (P<0.05), barrier function (P<0.05) and the diversity of gut microbiota (P<0.05) in the growing hens. The best performance was recorded among the hens fed 4.5 g/kg level of small peptide. Taken together, these results showed that small peptide supplementation could improve the economic value of growing hens by promoting growth rate, disease resistance, and the optimal amount of addition for Tianfu green shell laying hens was 4.5 g/kg.

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Section: Discussionmentioning

confidence: 99%

Effects of Small Peptide Supplementation on Growth Performance, Intestinal Barrier of Laying Hens During the Brooding and Growing Periods

et al. 2022

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“…Th1 cells are involved in the cellular immunity and secrete the effector Th1 type cytokine. So Th1 cytokine level and T‐lymphocyte proliferative activity can reflect the basic status of cellular immunity (Balenovi, Savi, Ekert, Jurinovi, & Ragland, ). Our data showed that, except 7 days after immunization, the Th1 type cytokine levels (IL‐2 and TNF‐α) in the VaBGs group at the other time points were significantly higher than those in the other two groups (FKC and PBS groups) ( p < .05).…”

Section: Discussionmentioning

confidence: 99%

Identification of fish source Vibrio alginolyticus and evaluation of its bacterial ghosts vaccine immune effects

Cao

Zhang

et al. 2018

MicrobiologyOpen

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Vibrio alginolyticus (V. alginolyticus) is a common pathogen for humans and marine aquatic animals. Vibriosis of marine aquatic animals, caused by V. alginolyticus, has become more prevalent globally in recent years. Hence, a safe and effective vaccine is urgently needed for the control of this disease. Here, the strain 16‐3 isolated from the large yellow croaker (Larimichthys crocea) suffered from canker was identified as V. alginolyticus based on morphological, biochemical, and 16S rDNA sequencing analysis. Then, recombinant temperature‐controlled lysis plasmid pBV220‐lysisE was electroporated into the strain 16‐3 to generate V. alginolyticus bacterial ghosts (VaBGs) by inducing lysis gene E expression, and the safety and immune effects of VaBGs were further investigated in mice and large yellow croaker. The results showed that VaBGs were as safe as formalin‐killed V. alginolyticus cells (FKC) to mice and fish. Compared with FKC and PBS groups, significant elevations of the serum agglutinating antibody titer, serum bactericidal activity, lymphocyte proliferative responses, and levels of four different cytokines (Th1 type: IL‐2, TNF‐α; Th2 type: IL‐4 and IL‐6) in serum were detected in the VaBGs group, indicating that a Th1/Th2‐mediated mixed immune response was elicited by the VaBGs. More importantly, after challenged with the parent strain 16‐3, VaBGs‐vaccinated mice and fish showed higher protection than FKC‐vaccinated mice, the relative percent of survival (RPS) being 60%, 66.7% and 40%, respectively. Taken together, this is the first demonstration that the newly constructed V. alginolyticus ghosts may be developed as a safe and effective vaccine against V. alginolyticus infection in aquaculture.

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“…Th1 cells are involved in cellular immunity and secrete the effector Th1-type cytokine. Thus, the Th1 cytokine level and T lymphocyte proliferative activity can reflect the basic status of cellular immunity 35 . Our data indicated that the Th1 type cytokine levels (IL-12 and IFN-γ) in the vaccination groups after immunization were significantly higher than in the control group (Fig.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity of a secreted, C-terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development

Ryan

Luke

et al. 2023

Sci Rep

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Both current live, attenuated, and killed virus vaccines for bovine viral diarrhea virus (BVDV) have their limitations. Here, we report the development of a BVDV subunit vaccine by (i) the expression of a secreted form of a recombinant E2 glycoprotein using BHK21 cells and (ii) determination of the immune responses in mice. The E2 glycoprotein was modified by deletion of the C-terminal transmembrane anchor domain and fusion to a V5 epitope tag. This allowed detection using anti-V5 monoclonal antibodies together with simple purification of the expressed, secreted, form of E2 from the cell media. Furthermore, we genetically fused green fluorescent protein (GFP) linked to E2 via a Thosea asigna virus 2A (T2A) ribosome skipping sequence thereby creating a self-processing polyprotein [GFP-T2A-BVDV-E2trunk-V5], producing discrete [GFP-T2A] and [E2trunk-V5] translation products: GFP fluorescence acts, therefore, as a surrogate marker of E2 expression, BALB/c mice were inoculated with [E2trunk-V5] purified from cell media and both humoral and cellular immune responses were observed. Our antigen expression system provides, therefore, both (i) a simple antigen purification protocol together with (ii) a feasible strategy for further, large-scale, production of vaccines.

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Abundance of IFN-α and IFN-γ gene transcripts and absence of IL-2 transcripts in the blood of chickens vaccinated with live or inactivated NDV

Cited by 9 publications

References 22 publications

Effects of Small Peptide Supplementation on Growth Performance, Intestinal Barrier of Laying Hens During the Brooding and Growing Periods

Effects of Small Peptide Supplementation on Growth Performance, Intestinal Barrier of Laying Hens During the Brooding and Growing Periods

Identification of fish source Vibrio alginolyticus and evaluation of its bacterial ghosts vaccine immune effects

Immunogenicity of a secreted, C-terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development

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