Abundance of the benthic dinoflagellate Prorocentrum and the diversity, distribution, and diarrhetic shellfish toxin production of Prorocentrum lima complex and P. caipirignum in Japan

Nishimura, Tomohiro; Uchida, Hajime; Noguchi, Ryoko; Suzuki, Toshiyuki; Funaki, Hiroshi; Ihara, Chiho; Hagino, K.; Arimitsu, Shingo; Tanii, Yuta; Abe, Shota; Hashimoto, Kana; Mimura, Katsuya; Tanaka, Kosuke; Yanagida, Ippei; Adachi, Masao

doi:10.1016/j.hal.2019.101687

Cited by 37 publications

(35 citation statements)

References 68 publications

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“…These morphological characteristics can vary among genetic lineages; hence, it is essential to determine the molecular identity of field specimens and cultured isolates for unambiguous taxonomic assignment (Nascimento et al, 2017;Durán-Riveroll et al, 2019). In the absence of comprehensive molecular genetic evidence, morphological "P. lima" can be interpreted as representing an unresolved species complex [P. lima species complex (PLSC)] (Zhang et al, 2015;Nishimura et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Associated Bacteria and Their Effects on Growth and Toxigenicity of the Dinoflagellate Prorocentrum lima Species Complex From Epibenthic Substrates Along Mexican Coasts

et al. 2020

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Bacteria Associated With Benthic Prorocentrum densities throughout the culture cycle. Antibiotic treatment did not impair growth or survival of the dinoflagellate, or apparently affect DSP toxin production. There was no significant correlation between Prorocentrum cell volume, growth rate, bacterial cell counts, or cellular toxin concentration over the entire time-series culture cycle. Benthic Prorocentrum and associated bacterial communities comprise highly diverse and characteristic microbiomes upon substrates, and among compartments in culture, but this study provides little evidence that allelochemical interactions among Prorocentrum cells and associated bacteria originating from epibenthic substrates play a definable role in growth and toxigenicity.

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Section: Introductionmentioning

confidence: 99%

Associated Bacteria and Their Effects on Growth and Toxigenicity of the Dinoflagellate Prorocentrum lima Species Complex From Epibenthic Substrates Along Mexican Coasts

et al. 2020

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“…Fraction with the highest purity and content of OA was 17 (>90%, 25.3 mg), for DTX1 fraction 19 (>35%, 11.6 mg) and for DTX1 analogue fraction 22 (>45%, 13.2 mg). Prorocentrum species produce different DSP content depending on the species and/or strains ( An et al, 2010 ; Aquino-Cruz et al, 2018 ; Ben-Gharbia et al, 2016 ; Lee et al, 2016 ; Luo et al, 2017 ; Nishimura et al, 2019 ; Nishimura et al, 2020 ; Pan et al, 2017 ). Previous studies on large-scale culture of P. lima and separation and purification of OA and DTX1 were published.…”

Section: Resultsmentioning

confidence: 99%

“…The dinoflagellates Prorocentrum ( P. fortii , P. lima , P. concavum and P. minimum ) and Dinophysis ( D. fortii , D. acuminate , D. acuta ) are the main producers of DSP toxins ( Aquino-Cruz et al, 2018 ; Bravo et al, 2001 ; Lee et al, 2016 ; Luo et al, 2017 ; Nishimura et al, 2019 ; Pan et al, 2017 ; Uchida et al, 2018 ). Okadaic acid (OA) and dinophysistoxin 1 and 2 (DTX1, DTX2) are the original DSP toxins.…”

Section: Introductionmentioning

confidence: 99%

Degradation of okadaic acid in seawater by UV/TiO2 photocatalysis – Proof of concept

Camacho‐Muñoz

Lawton

Edwards

2020

Science of The Total Environment

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The consumption of contaminated shellfish with marine toxins causes adverse socioeconomical, environmental and health impacts. The marine toxin okadaic acid (OA) provokes diarrhetic shellfish poisoning (DSP) syndrome characterized by severe gastrointestinal symptoms. Therefore, there is increasing interest in removing these toxins from the marine environment to protect shellfish harvesting sites. Photocatalysis is proposed as an efficient method to detoxify the marine environment. In this study, Prorocentrum lima was used to produce high purity DSP toxins, in particular OA, for degradation studies. The profiling, characterization and quantification of DSP toxins in the culture of P. lima were achieved by ultrahigh performance liquid chromatography coupled to quadrupole-time of flight mass spectrometry (UPLC-QTOF-MS E ) for accurate-mass full spectrum acquisition data. The effectiveness of UV/TiO 2 system to degrade OA in seawater was assessed in lab-scale experiments and identification of transformation products was proposed based on the data obtained during analysis by UPLC-QTOF-MS E . The detoxification potential of the UV/TiO 2 system was investigated using the phosphatase inhibition assay. Sufficient amount of high-purity OA (25 mg, >90% purity) was produced in-house for use in photocatalysis experiments by simple reversed-phase flash chromatography. Complete degradation of OA was observed in seawater after 30 min and 7.5 min in deionized water. The rate constants fitted with the pseudo-first order kinetic model (R 2 > 0.96). High-resolution mass spectrometry analysis of the photocatalyzed OA allowed tentative identification of four transformation products. Detoxification was achieved in parallel with the degradation of OA in deionized water and artificial ocean water (≤20 min) but not for seawater. Overall, results suggest that UV/TiO 2 photocatalysis can be an effective approach for degrading OA and their TPs in the marine environment. To the best of our knowledge, this is the first report on the use of photocatalysis to degrade marine toxins and its promising potential to protect shellfish harvesting sites.

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“…Because of this uncertainty in species identification, we choose to design and test a genus-level probe. Prorocentrum lima is another paraphyletic taxon, with at least four major clades recovered in its phylogenetic analyses with nine or more subclades [25,26]. Not all of the species from the Zhang et al study [25] were included in the Nishimura et al study [26] but in our ARB database, all are included and we identified eight major clades for which we designed probes.…”

Section: Resultsmentioning

confidence: 99%

Advances in the Detection of Toxic Algae Using Electrochemical Biosensors

Medlin

Gamella

Mengs³

et al. 2020

Biosensors

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Harmful algal blooms (HABs) are more frequent as climate changes and tropical toxic species move northward, especially along the Iberian Peninsula, a rich aquaculture area. Monitoring programs, detecting the presence of toxic algae before they bloom, are of paramount importance to protect ecosystems, aquaculture, human health and local economies. Rapid, reliable species identification methods using molecular barcodes coupled to biosensor detection tools have received increasing attention as an alternative to the legally required but impractical microscopic counting-based techniques. Our electrochemical detection system has improved, moving from conventional sandwich hybridization protocols using different redox mediators and signal probes with different labels to a novel strategy involving the recognition of RNA heteroduplexes by antibodies further labelled with bacterial antibody binding proteins conjugated with multiple enzyme molecules. Each change has increased sensitivity. A 150-fold signal increase has been produced with our newest protocol using magnetic microbeads (MBs) and amperometric detection at screen-printed carbon electrodes (SPCEs) to detect the target RNA of toxic species. We can detect as few as 10 cells L−1 for some species by using a fast (~2 h), simple (PCR-free) and cheap methodology (~2 EUR/determination) that will allow this methodology to be integrated into easy-to-use portable systems.

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Abundance of the benthic dinoflagellate Prorocentrum and the diversity, distribution, and diarrhetic shellfish toxin production of Prorocentrum lima complex and P. caipirignum in Japan

Cited by 37 publications

References 68 publications

Associated Bacteria and Their Effects on Growth and Toxigenicity of the Dinoflagellate Prorocentrum lima Species Complex From Epibenthic Substrates Along Mexican Coasts

Associated Bacteria and Their Effects on Growth and Toxigenicity of the Dinoflagellate Prorocentrum lima Species Complex From Epibenthic Substrates Along Mexican Coasts

Degradation of okadaic acid in seawater by UV/TiO2 photocatalysis – Proof of concept

Advances in the Detection of Toxic Algae Using Electrochemical Biosensors

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