Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant

Song, Kang; Suenaga, Toshikazu; Hamamoto, Aki; Satou, Kouichi; Riya, Shohei; Hosomi, Masaaki; Terada, Akihiko

doi:10.1016/j.jbiosc.2014.02.028

Cited by 42 publications

(32 citation statements)

References 52 publications

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“…The DNA abundances and mRNA transcription levels of functional genes indicate the degree to which their functional roles and reaction rates are related (Park et al 2010;Song et al 2014;Wang et al 2014). The biomass was periodically sampled throughout the MLE process.…”

Section: Microbial Community Analysesmentioning

confidence: 99%

“…The primers used in this study are summarized in Table S1. The DNA and mRNA levels of functional genes encoding ammonia oxidation (amoA) and nitrite reduction of heterotrophic bacteria (nirS and nirK) were quantified as reported elsewhere (Song et al 2014). Functional genes encoding N 2 O reduction (nosZ clade I) were quantified by real-time polymerase chain reaction (qPCR) with nosZ1 primers (reported in Henry et al 2006).…”

Section: Microbial Community Analysesmentioning

confidence: 99%

“…The PCR reactions contained 10 μL of THUN-DERBIRD ® SYBR ® qPCR Mix (Toyobo Co. Ltd., Life Science Department, Osaka, Japan), 0.8 μL of MgCl 2 , 0.2 μL of ddH 2 O, 5 μL of DNA sample template, and 2 μL of the forward and reverse primers. The other PCR conditions are reported elsewhere (Song et al 2014). Based on the 16S rRNA genes, the microbial community structures were investigated by the MiSeq Illumina sequencing technology as previously reported (Hori et al 2014).…”

Section: Microbial Community Analysesmentioning

confidence: 99%

“…Based on the 16S rRNA genes, the microbial community structures were investigated by the MiSeq Illumina sequencing technology as previously reported (Hori et al 2014). The diversity indices of the samples were calculated by the Shannon-Weiner index (H′) and the Evenness index (E) as previously reported (Song et al 2014). …”

Section: Microbial Community Analysesmentioning

confidence: 99%

“…This reaction is mediated by N 2 O reductase, encoded by nosZ genes. Although N 2 O production mechanisms during BNR have been extensively studied in physiological and biochemical levels, only a few studies investigated N 2 O reduction mechanism by transcription level of nosZ gene (Song et al 2014;Wang et al 2014). Furthermore, implication of nosZ transcription and microbial community into N 2 O emission has seldom been investigated.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effects of aeration and internal recycle flow on nitrous oxide emissions from a modified Ludzak–Ettinger process fed with glycerol

Song

Suenaga

Harper

et al. 2015

Environ Sci Pollut Res

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Nitrous oxide (N2O) is emitted from a modified Ludzak-Ettinger (MLE) process, as a primary activated sludge system, which requires mitigation. The effects of aeration rates and internal recycle flow (IRF) ratios on N2O emission were investigated in an MLE process fed with glycerol. Reducing the aeration rate from 1.5 to 0.5 L/min increased gaseous the N2O concentration from the aerobic tank and the dissolved N2O concentration in the anoxic tank by 54.4 and 53.4 %, respectively. During the period of higher aeration, the N2O-N conversion ratio was 0.9 % and the potential N2O reducers were predominantly Rhodobacter, which accounted for 21.8 % of the total population. Increasing the IRF ratio from 3.6 to 7.2 decreased the N2O emission rate from the aerobic tank and the dissolved N2O concentration in the anoxic tank by 56 and 48 %, respectively. This study suggests effective N2O mitigation strategies for MLE systems.

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Section: Microbial Community Analysesmentioning

confidence: 99%

Section: Microbial Community Analysesmentioning

confidence: 99%

Section: Microbial Community Analysesmentioning

confidence: 99%

Section: Microbial Community Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of aeration and internal recycle flow on nitrous oxide emissions from a modified Ludzak–Ettinger process fed with glycerol

Song

Suenaga

Harper

et al. 2015

Environ Sci Pollut Res

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Potential microbial functions and quorum sensing systems in partial nitritation and anammox processes

Feng

Sun

et al. 2021

Water Environment Research

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Diverse microbial communities coexist in the partial nitritation-anaerobic ammonium oxidation (PNA) process, in which nitrogen metabolism and information exchange are two important microbial interactions. In the PNA process, the existence of diverse microorganisms including nitrifiers, anammox bacteria, and heterotrophs makes it challenging to achieve a balanced relationship between anaerobic ammonium oxidation bacteria and ammonia oxidizing bacteria. In this study, potential microbial functions in nitrogen conversion and acyl-homoserine lactones (AHLs)-based quorum sensing (QS) in PNA processes were examined. Candidatus_Kuenenia and Nitrosomonas were the key functional bacteria responsible for PNA, while Nitrospira was detected as the dominant nitrite oxidizing bacteria (NOB). Heterotrophs containing nxr might play a similar function to NOB. The AHLs-QS system was an important microbial communication pathway in PNA systems. N-octanoyl-L-homoserine lactone, N-decanoyl homoserine lactone, and N-dodecanoyl homoserine lactone were the main AHLs, which might be synthesized by nitrogen converting microorganisms and heterotrophs. However, only heterotrophs had the potential to sense and degrade AHLs, such as Saccharophagus (sensing) and Leptospira (degradation). These results provide comprehensive information about the possible microbial functions and interactions in the PNA system and clues for system optimization from a microbial perspective. © 2021 Water Environment Federation • Practitioner points•Potential functions of anammox bacteria, nitrifiers, and heterotrophs were revealed.•Diverse nitrogen conversion and AHLs-quorum sensing related genes were detected.•Anammox bacteria and AOB played important roles in the AHLs synthesis process.•Heterotrophs could sense and degrade AHLs during information exchange.

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Effects of Nitrogen Input on Community Structure of the Denitrifying Bacteria with Nitrous Oxide Reductase Gene (nosZ I): a Long-Term Pond Experiment

Zhou

Kong

et al. 2022

Microb Ecol

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Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant

Cited by 42 publications

References 52 publications

Effects of aeration and internal recycle flow on nitrous oxide emissions from a modified Ludzak–Ettinger process fed with glycerol

Effects of aeration and internal recycle flow on nitrous oxide emissions from a modified Ludzak–Ettinger process fed with glycerol

Potential microbial functions and quorum sensing systems in partial nitritation and anammox processes

Effects of Nitrogen Input on Community Structure of the Denitrifying Bacteria with Nitrous Oxide Reductase Gene (nosZ I): a Long-Term Pond Experiment

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