Abundances of vitellogenin and heat shock protein 90 during ovarian and embryonic development of Exopalaemon carinicauda

Liang, Junping; Zhang, Weili; Li, Hui; Li, Jitao

doi:10.1016/j.anireprosci.2020.106633

Cited by 14 publications

(19 citation statements)

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“…Vitellogenesis originates in both the hepatopancreas and ovary in most crustacean (Zmora et al, 2007). Liang et al, found that vitellogenin of E. carinicauda is synthesized both in the ovary and hepatopancreas (Liang et al, 2020). In this study, the mRNA expression level of Vtgs and vitellogenin receptor (Vtgr) in the stage II were significantly higher than that in the stage I.…”

Section: Discussionmentioning

confidence: 43%

“…However, aquaculture production and large-scale cultivation are still restricted by the supply of high-quality broodstock. In addition to its economic value, E. carinicauda is a useful experimental organism in the field of crustacean developmental biology due to its transparent body, large egg, and high reproductive capacity, and short reproductive cycle of only 2-3 months (Yuan et al, 2017;Liang et al, 2020;Wang et al, 2020). The female shrimps generate at least three consecutive ovarian development during reproductive stage, but the ovaries of some shrimps gradually atrophy into cystic tubes after first ovarian mature.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have reported the different ovarian maturation stages of E. carinicauda (Wang, 1987); the ovary size and color undergo visible changes during ovarian maturation. Researchers have cloned, expressed, and studied the functions of ovarian maturation-related genes in E. carinicauda, including those that encode farnesoic acid O-methyltransferase (FAMeT), vitellogenin (Vtg) and heat shock protein 90 (Duan et al, 2014;Li et al, 2016;Liang et al, 2020). These genes have also been studied in the Palaemonidae family, including Macrobrachium nipponense (Bai et al, 2016) and Macrobrachium rosenbergii (Guo et al, 2019a;Qian and Liu, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

Wang

Li²,

Ge³

et al. 2022

Front. Mar. Sci.

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The ridgetail white shrimp Exopalaemon carinicauda has the potential to be used as a useful experimental organism in the field of crustacean research due to its transparent body, large egg, and short reproductive cycle. However, little is known about the regulatory mechanisms of ovarian maturation in E. carinicauda. In this study, we applied PacBio single-molecule long-read sequencing technology to unveil the whole transcriptome landscape of Exopalaemon carinicauda to better understand the molecular mechanisms of ovarian development. A total of 49.05 G polymerase read bases were generated, finally, 15, 151 unigenes were successfully identified with a mean length of 4, 257 bp and an N50 of 4, 884 bp from ovary tissues (five different ovarian developmental stages). A total of 14, 007 unigenes were successfully annotated in at least one public database. In addition, 8, 861 coding sequences, 4, 594 lncRNAs and 895 transcription factors were identified. Furthermore, we performed RNA-Seq analysis combined with Iso-Seq results to investigate ovarian maturation regulation mechanism and validated the results by quantitative real-time PCR. We annotated five vitellogenin isoforms in the full-length transcriptome, meanwhile these five genes and vitellogenin receptor suggested an important role during previtellogenesis of E. carinicauda. Genes were involved in PI3K-Akt signaling pathway, retinol metabolism, cell cycle and hedgehog signaling pathway, which showed obvious associations with ovarian maturation of E. carinicauda. We identified 40 DEGs, such as forkhead box protein L2, which may be related to ovarian development in the comparisons. The expression profiles of genes such as ecdysone receptor, ecdysone-induced protein 74EF, ecdysone inducible gene E75 and post-molt protein suggest that they were significantly associated with reproductive molting in the ovarian mature stage. In conclusion, the present study identified important genes and pathways involved in ovarian maturation, which might be useful for studying the reproductive regulation and mechanisms of ovarian maturation in E. carinicauda.

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Section: Discussionmentioning

confidence: 43%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

Wang

Li²,

Ge³

et al. 2022

Front. Mar. Sci.

Self Cite

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“…Various studies have found that the up‐regulation or down‐regulation of many genes can affect the accumulation of yolk in crustaceans. For example, with the help of the vitellogenin receptor ( VgR ), vitellogenin ( Vg ) is transferred from the hemolymph to developing oocytes (Bai et al, 2015; Liang et al, 2016), and the interference with VgR will down‐regulate the expression of Vg in the ovary. This results in the decreasing content of vitellogenin in the ovary, which effectively delays the development of oocytes and prolongates the ovarian cycle (Bai et al, 2016; Tiu et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…The annual output is about 50,000 tons (Deng et al, 2021; Ma et al, 2019; Wang et al, 2021). At present, the cultured seedlings of this shrimp are mainly obtained by collecting shrimp seedlings from natural sea areas or by the self‐propagation of female parents with eggs (Li et al, 2019; Liang et al, 2020). The large‐scale cultivation of seedlings has not yet been established, because of the great variation in the ovarian development of female parents.…”

Section: Introductionmentioning

confidence: 99%

Cloning and function of the cyclin‐dependent kinase 9 gene in ovarian development of the ridgetail white prawn, Exopalaemon carinicauda

Zhang¹,

Li²,

Zheng³

et al. 2022

Aquaculture Research

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To investigate the key genes affecting ovarian development in the ridgetail white prawn, Exopalaemon carinicauda, we cloned the full‐length cDNA of the CDK9 gene and discussed its role in ovarian development. Our results show that the gene has an open reading frame of 1155 bp, encoded 383 amino acids, and it has a conserved cyclin binding motif S_TKc. The open reading frame of CDK9 was expressed as a 43.96 Kda molecular weight protein. The phylogenetic tree analysis showed that the CDK9 gene has the highest homology with the CDK9 genes of Penaeus vannamei and Penaeus japonicus. The results of qRT‐PCR show that the CDK9 gene was also expressed in the eye stalk, stomach, heart, gill, intestine, muscle, ovary, hepatopancreas and ventral cord. The expression level was highest in the ovary, which was significantly different from the other tissues (p < 0.05). In the five stages of ovarian development, the expression level of the CDK9 gene was highest in stage I, which was significantly different from the other stages (p < 0.05). In situ hybridization tests showed strong expression signals in the membrane, cytoplasm and nucleus of oocytes and previtellogenic oocytes. Nevertheless, following the proliferation of oocytes, the expression signals became much stronger in the oocyte membrane and in the whole follicular cells located in the interstitial space. The RNA interference results show that the expression of the CDK9 gene significantly affects the expression of the Vg and VgR genes. Our findings suggest that CDK9 plays an important role in the synthesis and transportation of vitellin.

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Characterization and expression analysis of heat shock proteins HfHspc1 and HfHspc4 in Haemaphysalis flava ticks

Li,

Wang,

Feng

et al. 2024

Parasitol Res

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Abundances of vitellogenin and heat shock protein 90 during ovarian and embryonic development of Exopalaemon carinicauda

Cited by 14 publications

References 49 publications

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

Cloning and function of the cyclin‐dependent kinase 9 gene in ovarian development of the ridgetail white prawn, Exopalaemon carinicauda

Characterization and expression analysis of heat shock proteins HfHspc1 and HfHspc4 in Haemaphysalis flava ticks

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