AC electrokinetic manipulation of DNA

Wälti, Christoph; Germishuizen, W. A.; Tosch, P.; Kaminski, Clemens F.; Davies, A. G.

doi:10.1088/0022-3727/40/1/s16

Cited by 13 publications

(15 citation statements)

References 43 publications

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“…Note that the extension at the peak frequency is close to complete. 540 Unsurprisingly, the bottom panel of Figure 69 shows that the DNA stretching increases with the strength of the electric field. 540 The dielectrophoretic stretching is also a strong function of the pH of the buffer, with strong stretching at a pH of 8 and no stretching at a pH of 4 or 10.…”

Section: Dna Stretchingmentioning

confidence: 99%

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching

et al. 2012

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Section: Dna Stretchingmentioning

confidence: 99%

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching

et al. 2012

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“…AC electrokinetics provides a powerful mechanism for both positioning and inducing conformational changes in molecules with only minimal instrumentation. 178,187 Thus, the above discussed approaches along with numerous other AC field induced DNA manipulation techniques have certainly enabled the development of simple point-of-care platforms for rapid DNA detection.…”

Section: Applications In Dna Trapping and Analysismentioning

confidence: 99%

Alternating current electrohydrodynamics in microsystems: Pushing biomolecules and cells around on surfaces

et al. 2015

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Electrohydrodynamics (EHD) deals with the fluid motion induced by an electric field. This phenomenon originally developed in physical science, and engineering is currently experiencing a renaissance in microfluidics. Investigations by Taylor on Gilbert's theory proposed in 1600 have evolved to include multiple contributions including the promising effects arising from electric field interactions with cells and particles to influence their behaviour on electrode surfaces. Theoretical modelling of electric fields in microsystems and the ability to determine shear forces have certainly reached an advanced state. The ability to deftly manipulate microscopic fluid flow in bulk fluid and at solid/liquid interfaces has enabled the controlled assembly, coagulation, or removal of microstructures, nanostructures, cells, and molecules on surfaces. Furthermore, the ability of electrohydrodynamics to generate fluid flow using surface shear forces generated within nanometers from the surface and their application in bioassays has led to recent advancements in biomolecule, vesicle and cellular detection across different length scales. With the integration of Alternating Current Electrohydrodynamics (AC-EHD) in cellular and molecular assays proving to be highly fruitful, challenges still remain with respect to understanding the discrepancies between each of the associated ac-induced fluid flow phenomena, extending their utility towards clinical diagnostic development, and utilising them in tandem as a standard tool for disease monitoring. In this regard, this article will review the history of electrohydrodynamics, followed by some of the recent developments in the field including a new dimension of electrohydrodynamics that deals with the utilization of surface shear forces for the manipulation of biological cells or molecules on electrode surfaces. Recent advances and challenges in the use of electrohydrodynamic forces such as dielectrophoresis and ac electrosmosis for the detection of biological analytes are also reviewed. Additionally, the fundamental mechanisms of fluid flow using electrohydrodynamics forces, which are still evolving, are reviewed. Challenges and future directions are discussed from the perspective of both fundamental understanding and potential applications of these nanoscaled shear forces in diagnostics.

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“…For example, it has been already used to study DNA molecules [41][42][43] and actin filaments [2,44]. This method can also be readily combined with other positioning methods.…”

Section: Particle Assemblymentioning

confidence: 99%

Double‐layer polarization of a non‐conducting particle in an alternating current field with applications to dielectrophoresis

Zhao

2011

Electrophoresis

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Dielectrophoresis is becoming one of the most important techniques in particle manipulation including particle separation, particle assembly, and biomolecule characterization. Understanding dielectrophoretic properties of particles is a key step toward effective and efficient particle manipulation. Theoretical studies of polarization of a particle can help to understand experimental observations and also go beyond to develop a predictive theory to guide the experimental design. This article discusses recent theoretical advances in the polarization of a dielectric particle, in particular, the polarization of the electric double layer. The double-layer polarization is critical to determine particle dynamics in dielectrophoresis. The dipole moment characterizing the strength of this polarization depends on the double-layer thickness, the electric field frequency, the particle's surface charge, and other surface's properties (Pohl, H. A., Dielectrophoresis, Cambridge University Press, New York 1978). After a brief review of the mathematical model, the focus is on the following problems: (i) the polarization of a spherical particle; (ii) the polarization of an elongated cylindrical particle; (iii) the effect of the slip on the polarization of a particle. The double-layer polarization is examined here in the context of high-frequency and low-frequency dispersions induced by surface conduction and diffusion, respectively.

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AC electrokinetic manipulation of DNA

Cited by 13 publications

References 43 publications

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching

Alternating current electrohydrodynamics in microsystems: Pushing biomolecules and cells around on surfaces

Double‐layer polarization of a non‐conducting particle in an alternating current field with applications to dielectrophoresis

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