AC-electrophoretic deposition of glucose oxidase

Ammam, Malika; Fransaer, Jan

doi:10.1016/j.bios.2009.06.036

Cited by 54 publications

(36 citation statements)

References 30 publications

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“…Recent developments have shown the application of unbalanced AC fields of sufficient amplitude to achieve deposition of enzymes and bacteria (Ammam & Fransaer 2009;Neirinck et al 2009b). Ammam & Fransaer (2009), for example, deposited glucose oxidase from water onto a platinum electrode. Glucose oxidase layers with a thickness of 7 mm could be obtained after 20 min of EPD.…”

Section: Other Applications Of Epd In Nanobiotechnologymentioning

confidence: 99%

Electrophoretic deposition of biomaterials

Boccaccını

Keim

et al. 2010

J. R. Soc. Interface.

621

412

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Electrophoretic deposition (EPD) is attracting increasing attention as an effective technique for the processing of biomaterials, specifically bioactive coatings and biomedical nanostructures. The well-known advantages of EPD for the production of a wide range of microstructures and nanostructures as well as unique and complex material combinations are being exploited, starting from well-dispersed suspensions of biomaterials in particulate form (microsized and nanoscale particles, nanotubes, nanoplatelets). EPD of biological entities such as enzymes, bacteria and cells is also being investigated. The review presents a comprehensive summary and discussion of relevant recent work on EPD describing the specific application of the technique in the processing of several biomaterials, focusing on (i) conventional bioactive (inorganic) coatings, e.g. hydroxyapatite or bioactive glass coatings on orthopaedic implants, and (ii) biomedical nanostructures, including biopolymer-ceramic nanocomposites, carbon nanotube coatings, tissue engineering scaffolds, deposition of proteins and other biological entities for sensors and advanced functional coatings. It is the intention to inform the reader on how EPD has become an important tool in advanced biomaterials processing, as a convenient alternative to conventional methods, and to present the potential of the technique to manipulate and control the deposition of a range of nanomaterials of interest in the biomedical and biotechnology fields.

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Section: Other Applications Of Epd In Nanobiotechnologymentioning

confidence: 99%

Electrophoretic deposition of biomaterials

Boccaccını

Keim

et al. 2010

J. R. Soc. Interface.

621

412

View full text Add to dashboard Cite

show abstract

“…It is used, e.g., for the transport of water microdroplets in a nematic liquid crystal by AC electric fields with frequencies of 100 Hz or below [36]. The deposition of proteins by triangular, unsymmetric fields at 30 Hz and at amplitudes of 150 V or more is referred to as "AC electrophoretic deposition" [37]. The term "AC electrophoresis" can also be found as a reference to observations in AC electrokinetic experiments on carbon nanotube alignment and attraction towards electrodes [38,39].…”

Section: Discussionmentioning

confidence: 99%

AC electrokinetic immobilization of organic dye molecules

et al. 2020

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The application of inhomogeneous AC electric fields for molecular immobilization is a very fast and simple method that does not require any adaptions to the molecule's functional groups or charges. Here, the method is applied to a completely new category of molecules: small organic fluorescence dyes, whose dimensions amount to only 1 nm or even less. The presented setup and the electric field parameters used allow immobilization of dye molecules on the whole electrode surface as opposed to pure dielectrophoretic applications, where molecules are attracted only to regions of high electric field gradients, i.e., to the electrode tips and edges. In addition to dielectrophoresis and AC electrokinetic flow, molecular scale interactions and electrophoresis at short time scales are discussed as further mechanisms leading to migration and immobilization of the molecules.

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“…Quantification of glucose in submicroliter volumes of fluid in a microplate was previously attempted both in food sample (Vermeir et al 2007) and biological fluid (Ciantar et al 2002) using commercially available enzyme assay kits to obtain glucose concentration intensity. In performing glucose concentration detection, the most commonly used biorecognition element is immobilized glucose oxidase (Ivnitski et al 2008;Ammam and Fransaer 2009). Glucose detection is generally performed using either electrochemical methods (Garcia and Henry 2004) or optical absorbance methods (Grumann et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Rapid glucose concentration detection utilizing disposable integrated microfluidic chip

Hou

Wang

Chang

et al. 2011

Microfluid Nanofluid

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A novel three-dimensional (3D) disposable glucose concentration detection chip is presented. The chip comprises a four-layer polymethyl methacrylate (PMMA) structure and is fabricated using a commercial CO 2 laser and a hot-press bonding technique. In the proposed device, the glucose solution is injected into a double parallel connection micromixer (DPCM) and is mixed with DNS reagent by means of a self-rotation effect. An experimental platform has been created for multiple reaction process by integrating chip and micro-heater. The fluid streams exiting the two circular mixing chambers of the DPCM are then combined and mixed further at a T-type microchannel outlet before passing to a collection chamber. Numerical simulations are performed to analyze the vortex streamline distribution within the DPCM and to estimate the mixing performance. The numerical results show that a mixing efficiency as high as 92.5% can be obtained at low Reynolds numbers (Re = 12). It is found a good linear relation of R 2 = 0.9953 from the chip detection method comparing to the traditional method of R 2 = 0.9976 at DNS reagent and glucose solution volume ratio of 1:1. In addition, the experimental results show that the accuracy of the glucose concentration measurements obtained using the proposed microfluidic chip is comparable with that of the measurements obtained using a conventional large-scale detection method. Overall, the results presented in this study indicate that the DPCM chip provides a rapid and low-cost means of detecting the concentration of glucose solutions.

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AC-electrophoretic deposition of glucose oxidase

Cited by 54 publications

References 30 publications

Electrophoretic deposition of biomaterials

Electrophoretic deposition of biomaterials

AC electrokinetic immobilization of organic dye molecules

Rapid glucose concentration detection utilizing disposable integrated microfluidic chip

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