ac field enhanced protein crystallization

Hou, Diana; Chang, Hsueh‐Chia

doi:10.1063/1.2938887

Cited by 58 publications

(72 citation statements)

References 17 publications

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“…With a 3-mm patterned gap on the graphene film, the applied voltage resulted in an electric field strength in the range of 0.4 V/mm to 0.6 V/mm, which is similar to a range reported in the literature [4]. As shown in Figure 5, Figures S1 and S2, the presence of an applied voltage resulted in an increased rate of protein nucleation and growth, which is consistent with previous literature reports [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Interestingly, these trends were only significant at short times.…”

Section: Resultssupporting

confidence: 81%

“…These strategies have also explored the effects of constant (DC) [5][6][7][13][14][15][16][17][20][21][22] and alternating (AC) electric fields [8][9][10][11][12][16][17][18]. However, the benefits observed for the various electrode arrangements and crystallization setups reported to date have been limited by the need to manually harvest crystals for subsequent diffraction analysis.…”

Section: Introductionmentioning

confidence: 99%

“…Electrocrystallization platforms have been reported for a variety of crystallization schemes, including batch [6][7][8][9][10][11][14][15][16][17][18][19][20], vapor diffusion [5,[13][14][15][16], and counter-diffusion [21], taking advantage of both internal [6,7,[12][13][14][19][20][21][22] and external electric fields [5,[8][9][10]15,18,23]. These strategies have also explored the effects of constant (DC) [5][6][7][13][14][15][16][17][20][21][22] and alternating (AC) electric fields [8][9][10]...…”

Section: Introductionmentioning

confidence: 99%

“…The presence of an electric field during protein crystallization has been shown to affect both the rate of nucleation and the rate of crystal growth by controlling the local concentration and concentration gradients of proteins and the associated crystallization reagents [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. These methods have the potential to improve the success rate associated with protein crystallogenesis and enhance our understanding of the structure-function relationship in challenging biomacromolecular targets.…”

Section: Introductionmentioning

confidence: 99%

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A Graphene-Based Microfluidic Platform for Electrocrystallization and In Situ X-ray Diffraction

et al. 2018

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Here, we describe a novel microfluidic platform for use in electrocrystallization experiments. The device incorporates ultra-thin graphene-based films as electrodes and as X-ray transparent windows to enable in situ X-ray diffraction analysis. Furthermore, large-area graphene films serve as a gas barrier, creating a stable sample environment over time. We characterize different methods for fabricating graphene electrodes, and validate the electrical capabilities of our device through the use of methyl viologen, a redox-sensitive dye. Proof-of-concept electrocrystallization experiments using an internal electric field at constant potential were performed using hen egg-white lysozyme (HEWL) as a model system. We observed faster nucleation and crystal growth, as well as a higher signal-to-noise for diffraction data obtained from crystals prepared in the presence of an applied electric field. Although this work is focused on the electrocrystallization of proteins for structural biology, we anticipate that this technology should also find utility in a broad range of both X-ray technologies and other applications of microfluidic technology.

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Section: Resultssupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Graphene-Based Microfluidic Platform for Electrocrystallization and In Situ X-ray Diffraction

et al. 2018

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“…To apply an ac-current injection field during protein crystallization, Hou and Chang [47] have constructed interdigitated and quadrupole Ti/Au electrodes to study the competition between gel and crystal formation at different voltages and frequencies. While applying EFs, there occurs a reduction in the nucleation site numbers, accompanied by a rapid increase in lysozyme crystal size.…”

Section: Crystallization In the Presence Of Electric Fields (Efs)mentioning

confidence: 99%

Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields

Nanev

2017

Crystals

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Three-dimensional protein molecule structures are essential for acquiring a deeper insight of the human genome, and for developing novel protein-based pharmaceuticals. X-ray diffraction studies of such structures require well-diffracting protein crystals. A set of external physical factors may promote and direct protein crystallization so that crystals obtained are useful for X-ray studies. Application of electric fields aids control over protein crystal size and diffraction quality. Protein crystal nucleation and growth in the presence of electric fields are reviewed. A notion of mesoscopic level of impact on the protein crystallization exercised by an electric field is also considered.Keywords: protein crystallization; classical and two-step nucleation mechanisms; impact of electric fields on the protein crystallization; external and internal electric fields; number density; size and quality of protein crystals

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Microfluidic Devices for Bioapplications

Yeo

Chang

Chan

et al. 2010

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521

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Harnessing the ability to precisely and reproducibly actuate fluids and manipulate bioparticles such as DNA, cells, and molecules at the microscale, microfluidics is a powerful tool that is currently revolutionizing chemical and biological analysis by replicating laboratory bench-top technology on a miniature chip-scale device, thus allowing assays to be carried out at a fraction of the time and cost while affording portability and field-use capability. Emerging from a decade of research and development in microfluidic technology are a wide range of promising laboratory and consumer biotechnological applications from microscale genetic and proteomic analysis kits, cell culture and manipulation platforms, biosensors, and pathogen detection systems to point-of-care diagnostic devices, high-throughput combinatorial drug screening platforms, schemes for targeted drug delivery and advanced therapeutics, and novel biomaterials synthesis for tissue engineering. The developments associated with these technological advances along with their respective applications to date are reviewed from a broad perspective and possible future directions that could arise from the current state of the art are discussed.

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ac field enhanced protein crystallization

Cited by 58 publications

References 17 publications

A Graphene-Based Microfluidic Platform for Electrocrystallization and In Situ X-ray Diffraction

A Graphene-Based Microfluidic Platform for Electrocrystallization and In Situ X-ray Diffraction

Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields

Microfluidic Devices for Bioapplications

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