AC4 protein of tomato leaf curl Palampur virus is an RNA silencing suppressor and a pathogenicity determinant

Kulshreshtha, Aditya; Kumar, Yogesh; Roshan, Poonam; Bhattacharjee, Bipasha; Mukherjee, Sunil Kumar; Hallan, Vipin

doi:10.1016/j.micpath.2019.103636

Cited by 10 publications

(5 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When a plant is infected with virus, the host activates defense mechanisms to prevent the virus from replicating and spreading to other healthy parts. , Using Nicotiana benthamiana as the host, the effect of compound B2 on the expression and spread of PVY-GFP was evaluated. As can be seen from Figure A, 1% agarose gel electrophoresis exhibited specific bands corresponding to a size of 1000 bp, which is consistent with the size of PVY CP.…”

Section: Results and Discussionmentioning

confidence: 99%

Development and Mechanism Investigation of Novel Thioacetalized Indoles as Antiphytoviral Agents

Zhao,

He,

Shen

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Potato virus Y (PVY) is a highly destructive pathogen that infects Solanum tuberosumvL., commonly known as potato, a crop that produces one of the most crucial food staples of the world. The PVY viral infection can considerably reduce the yield and quality of potatoes, thereby causing significant economic ramifications. Given the unsatisfactory performance of commercially available antiviral agents against PVY, we synthesized a series of novel indole-derived compounds followed by their bioevaluation and investigation of the mechanisms governing their anti-PVY activity. These indole-based derivatives contain dithioacetal as a key chemical moiety, and most of them exhibit promising anti-PVY activities. In particular, compound B2 displays remarkable in vivo protective and inactivating properties, with half-maximal effective concentration (EC 50 ) values of 209.3 and 113.0 μg/mL, respectively, in stark contrast to commercial agents such as ningnanmycin (EC 50 = 281.4 and 136.3 μg/mL, respectively) and ribavirin (EC 50 = 744.8 and 655.4 μg/mL, respectively). The mechanism using which B2 enhances plant immune response to protect plants from PVY is elucidated using enzyme activity tests, real-time quantitative polymerase chain reaction (RT-qPCR), and proteomics techniques. This study aims to pave the way for developing candidate pesticides and related molecules using antiphytoviral activity.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Development and Mechanism Investigation of Novel Thioacetalized Indoles as Antiphytoviral Agents

Zhao,

He,

Shen

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…The most typical VSR is CMV 2b. Other recently identified VSRs include SVBV P6 ( Feng et al, 2018 ), HPWMoV P7 and P8 ( Gupta et al, 2018 , 2019 ), apple geminivirus V2 ( Zhan et al, 2018 ), raspberry bushy dwarf virus 1b ( Masamichi et al, 2019 ), tomato leaf curl Palampur virus AC4 ( Kulshreshtha et al, 2019 ) and so on, all aggravating the pathogenicity of heterologous virus PVX. In our study, we found that P28 exacerbated significantly the symptoms of PVX, indicating that P28 might play an important role in pathogenicity or virulence.…”

Section: Discussionmentioning

confidence: 99%

Identification of Silencing Suppressor Protein Encoded by Strawberry Mottle Virus

Fan

Gao

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Strawberry mottle virus (SMoV) is associated with strawberry decline disease, causing losses to fruit yield and quality. In this study, using a screening system that enables detection of both local and systemic plant host (RNA silencing) defense responses, we found that Pro2Glu and P28, encoded by SMoV RNA2 genome, functioned to suppress local and systemic RNA silencing triggered by single- but not double-stranded GFP RNA. Subcellular localization assay revealed that both Pro2Glu and P28 were localized to nucleus and cytoplasm. The deletion of 11 amino acid residues at the C-terminus destabilized Pro2Glu protein, and the disruption of two conserved GW motifs deprived Pro2Glu of ability to suppress RNA silencing. Additionally, SMoV Pro2Glu and P28 enhanced the accumulation of potato virus X (PVX) in Nicotiana benthamiana 22 days post-infiltration, and P28 exacerbated significantly the symptoms of PVX. Collectively, these data indicate that the genome of SMoV RNA2 encodes two suppressors of RNA silencing. This is the first identification of a stramovirus suppressor of RNA silencing.

show abstract

“…In general, many studies have shown that C1 and C2 proteins encoded by different geminiviruses can suppress silencing (Table 1 and Figure 1). Being pathogenicity determinants of geminiviruses, both V2 and C4 proteins also exhibit VSR activity [215,[218][219][220][221][222][223][224][225][226][227][228].…”

Section: Order Geplafuvirales Family Geminiviridaementioning

confidence: 99%

“…To counteract TGS, C4 suppresses SAM synthetase enzyme activity and interacts with AGO4 to eliminate viral genome methylation [210,214,215]. In addition, some C4 proteins specifically interfere with the cell-to-cell and/or systemic spread of silencing [217,223,227,229]. Particularly, to counteract the intercellular spread of the RNA silencing signal, the TYLCV-encoded C4 protein interacts with BARELY ANY MERISTEM (BAM) 1 and 2 proteins, which are plasma membrane-localized receptor-like kinases [216].…”

Section: Order Geplafuvirales Family Geminiviridaementioning

confidence: 99%

Role of Plant Virus Movement Proteins in Suppression of Host RNAi Defense

Atabekova

Solovieva

Chergintsev

et al. 2023

IJMS

View full text Add to dashboard Cite

One of the systems of plant defense against viral infection is RNA silencing, or RNA interference (RNAi), in which small RNAs derived from viral genomic RNAs and/or mRNAs serve as guides to target an Argonaute nuclease (AGO) to virus-specific RNAs. Complementary base pairing between the small interfering RNA incorporated into the AGO-based protein complex and viral RNA results in the target cleavage or translational repression. As a counter-defensive strategy, viruses have evolved to acquire viral silencing suppressors (VSRs) to inhibit the host plant RNAi pathway. Plant virus VSR proteins use multiple mechanisms to inhibit silencing. VSRs are often multifunctional proteins that perform additional functions in the virus infection cycle, particularly, cell-to-cell movement, genome encapsidation, or replication. This paper summarizes the available data on the proteins with dual VSR/movement protein activity used by plant viruses of nine orders to override the protective silencing response and reviews the different molecular mechanisms employed by these proteins to suppress RNAi.

show abstract

AC4 protein of tomato leaf curl Palampur virus is an RNA silencing suppressor and a pathogenicity determinant

Cited by 10 publications

References 50 publications

Development and Mechanism Investigation of Novel Thioacetalized Indoles as Antiphytoviral Agents

Development and Mechanism Investigation of Novel Thioacetalized Indoles as Antiphytoviral Agents

Identification of Silencing Suppressor Protein Encoded by Strawberry Mottle Virus

Role of Plant Virus Movement Proteins in Suppression of Host RNAi Defense

Contact Info

Product

Resources

About