Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

Abstract

Background: Autoclaving is used to eliminate contamination during tissue culturing, however, it is a complicated process, time-consuming and costly. Chemical sterilization of tissue culture can effectively eliminate contamination, is a simple procedure, and cost effective. However, studies on the chemical sterilization mostly focus on bud induction, while the effects of chemical sterilization overall process of tissue culture, including bud induction, proliferation, and rooting, remain to be determined. Here, we investigate the effect of chemical sterilization on bud induction, proliferation, and rooting of Acacia mangium × A. auriculiformis.Results: The results showed that chlorothalonil (0.2 g/L) was a suitable chemical sterilant, and bud induction medium was 1/8 Murashige and Skoog medium + agar 7 g/L + chlorothalonil 0.2 g/L + 6-benzylaminopurine 0.5 mg/L The highest induction rate (99.54%) was observed in the third to fifth buds’ stem segments collected in October treated with 0.8 g/L carbendazim for 3 min, with a contamination rate of 0. The rooting medium was agar 7 g/L + chlorothalonil 0.2 g/L+ indolebutyric acid 1.5 mg/L + naphthylacetic acid 0.5 mg/L, and the rooting rate was 97.62%. The proliferation rate and subculture duration showed a positive correlation, while the proliferation rate was 3.58 times higher at the fourth subculture rooting. Conclusions: Our results suggest that chlorothalonil can effectively replace autoclaving during bud induction, proliferation, and rooting of A. mangium × A. auriculiformis. The findings of this study provide technical support for rapid seedlings propagation, accelerates the breeding process of Acacia, and can be applied in other tree species.

Xanthan gum and sodium hypochlorite in vitro rooting of Gerbera hybrida cv. Essandre

“…The use of autoclaving for the sterilization of culture media, glassware, and utensils used in micropropagation is another factor that helps increase costs, due to electric energy consumption and equipment maTntenance expenses, along with the fact that it requires more work hours (Pais et al, 2016;da Costa Urtiga, 2019: Lu et al, 2021.…”

Season, basal media and plant growth regulators effect in wood plant in vitro propagation: a comprehensive review

“…Efforts to increase the propagation of finger cultures have been widely reported, including rapid tissue culture for the preparation clones [19], [33], chances of in vitro propagation in woody plants [7,17], in vitro for clonal propagation [20] In vitro regeneration testing for shoot multiplication [37], Phloroglucinol and silver nitrate enhances axillary shoot proliferation in nodal explants [23], Effect of glutamine for high frequency in vitro regeneration [30], mapping barriers in tissue culture, [17,31], and low-cost tissue culture [32], Browning Treatment in Tissue Culture [27], Influence of explant collection period, anti-browning strategy and growth regulators composition [28]. Optimization in tissue culture techniques to obtain the most suitable protocol [29], is very dynamic and continuous must be carried out because it is strongly influenced by plant characteristics, the technology used, and activity targets.…”