Acanthamoeba spp. cysts storage in filter paper

Abstract

A new method of storage of the free-living amoeba, Acanthamoeba, is described. For this purpose, strips of filter paper were impregnated with cysts of amoebas cultivated axenically and transferred into a cryotube. The strips were dehydrated at 30 degrees C for 24 h and after storage at room temperature. The amoeba viability has been evaluated and the cysts remain viable and pure for at least 1 year. Then, our method has been efficient for the storage of cysts during the evaluated period.

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

“… 44 These contributions meet the urgent demand for faster detection techniques since late diagnosis is a factor contributing for the high mortality or visual sequelae of FLA infections. 1 , 2 , 6 , 8 Other Acanthamoeba wet-bench research is concerned with culture manipulation, as the proposal of strategies for prolonging culture storage 46 and removing contaminants from cultures. 47 Additional approaches included investigating the role of phosphate transporters in metabolism, 48 description of ultrastructural cyst wall morphology, 49 and genomic features.…”