Ação da l-arginina na evolução de retalhos cutâneos de ratos sob exposição à nicotina

Guimarães, Marcus Vinicius Thomé Nora; Moreira, Guilherme Henrique Gonçalves; Rocha, Luana Parminondi; Nicoluzzi, João Eduardo Leal; Souza, Carlos José Franco de; Repka, João Carlos Domingues

doi:10.1590/s0100-69912013000100009

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…Immunonutrition is defined as the use of specific nutrients in formulas which promote through different mechanisms of action, effects on the inflammatory and imune systems 4 , 10 , 14 , 22 .…”

Section: Introductionmentioning

confidence: 99%

Effect of Omega-3 Fatty Acid in the Healing Process of Colonic Anastomosis in Rats

Castilho

Campos

Mello

2015

ABCD, arq. bras. cir. dig.

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Background : The use of long-chain polyunsaturated fatty acids has been studied in the context of healing and tissue regeneration mainly due to its anti-inflammatory, immunoregulatory and antioncogenic properties. Previous studies have demonstrated beneficial effects with the use of enteral immunonutrition containing various farmaconutrients such as L-arginine, omega-3, trace elements, but the individual action of each component in the healing of colonic anastomosis remains unclear. Aim : To evaluate the influence of preoperative supplementation with omega-3 fatty acids on the healing of colonic anastomoses of well-nourished rats. Methods : Forty Wistar adult male rats, weighing 234.4±22.3 g were used. The animals were divided into two groups: the control group received for seven days olive oil rich in omega-9 oil through an orogastric tube, while the study group received isocaloric and isovolumetric omega-3 emulsion at a dose of 100 mg/kg/day, also for seven days. Both groups were submitted to two colotomies followed by anastomosis, in the right and left colon, respectively. Parameters evaluated included changes in body weight, anastomotic complications and mortality, as well as maximum tensile strength by using a tensiometer and collagen densitometry at the anastomotic site. Results : There were no differences in body weight or mortality and morbidity between groups. The value of the maximum tensile strength of the control group was 1.9±0.3 N and the study group 1.7±0.2, p=0.357. There was, however, a larger amount of type I collagen deposition in the study group (p=0.0126). The collagen maturation índex was 1.74±0.71 in the control group and 1.67±0.5 in the study group; p=0,719). Conclusions : Preoperative supplementation of omega-3 fatty acid in rats is associated with increased collagen deposition of type I fibers in colonic anastomoses on the 5th postoperative day. No differences were observed in the tensile strength or collagen maturation index.

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Section: Introductionmentioning

confidence: 99%

Effect of Omega-3 Fatty Acid in the Healing Process of Colonic Anastomosis in Rats

Castilho

Campos

Mello

2015

ABCD, arq. bras. cir. dig.

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“…As doses de nicotina de 2 mg/kg de peso do animal por 4 semanas de exposição simularam um quadro de tabagismo acentuado (GUIMARÃES et al, 2013).…”

Section: Discussionunclassified

Avaliação da Exposição e da Interrupção da Nicotina Durante a Cicatrização em Ratos Wistar

et al. 2015

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RESUMO:As evidências clínicas e experimentais demonstram que o uso da nicotina tem efeitos deletéricos sobre a cicatrização. Entretanto, muitas dúvidas ainda existem entre cirurgiões e equipes de saúde em relação ao tempo de interrupção que antecede esses procedimentos. Nesta pesquisa, os ratos Wistar foram divididos em 3 grupos (G1, G2 e G3) que receberam doses diárias de 2 mg/kg de nicotina subcutânea durante 4 semanas. O G1 não interrompeu o uso por dez dias após a cirurgia de retirada de retalho cutâneo; o G2 interrompeu o uso da nicotina uma semana antes da cirurgia; e o G3 interrompeu por duas semanas antes da cirurgia, não fazendo mais uso durante os dez dias após esse procedimento. A análise dos parâmetros de cicatrização na fase proliferativa foi feita através de microscopia de luz e corados pelo H.E. e colorações especiais para análise de colágeno (Tricrômico de Masson e Verhoeff ). Encontrou-se no G1 a presença de crosta fibrinoleucocitária, uma visível desorganização do colágeno e um deficiente tecido de granulação quando comparados aos G2 e G3. Dos escores médios dos três grupos obteve-se p=0,028 considerados significativos e no pós-teste Tukey-Kramer foram encontradas diferenças estatísticas significativas entre os G1 e G3 (p<0,05). Na análise do tecido de granulação (p=0,0008) e do colágeno (p=0,049) também se obteve diferença estatística significativa. Pode-se concluir que na interrupção por uma ou duas semanas obteve-se uma melhor e mais eficiente cicatrização, podendo, portanto, extrapolar esses resultados para orientação do pré e pós-cirúrgicos de pacientes tabagistas. PALAVRAS-CHAVE: Cicatrização; Colágeno; Nicotina.ABSTRACT: Clinical and experimental evidences show that the use of nicotine has deleterious effects on wound healing, although there are still many doubts among surgeons and health teams with regard to the interruption that should precede interventions. Wistar rats, divided into three groups (G1, G2 and G3), received daily subcutaneous doses of 2 mg/kg nicotine during 4 weeks. G1 did not interrupt use during 10 days after surgery; G2 interrupted nicotine use a week prior to surgery; G3 interrupted during two weeks before surgery and did not take nicotine during ten days after surgical procedure. Woundhealing parameters during the proliferation phase were analyzed by light microscopy and stained with HE and special stains for collagen analysis (Masson´s Trichromium and Verhoeff ). A fibrin-leukocyte crust, a visible disorder of collagen and a granulation tissue deficiency occurred in G1 when compared to G2 and G3. Average scores of the three groups (p=0.028) were significant and significant statistical differences between G1 and G3 existed (p<0.05) by Tukey-Kramer´s post-test. Statistically significant difference existed in the granulation Saúde e Pesquisa, Maringá (PR)

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“…In addition, all animals were photographed and evaluated by Scales standardized by the Paint-Autocad-2015 program and performed patchy patch analysis, recording in square centimeters the total area, area of viable vascularized tissue and area of necrotic tissue (Figs. 3 and 4) 16 .…”

Section: Methodsmentioning

confidence: 99%

Use of derived adipose stem cells to reduce complications of cutaneous scarring in smokers. An experimental model in rats

Martins

Oliveira

Lima³

et al. 2019

Acta Cir. Bras.

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Purpose To evaluate the use of adipose-derived stem cells (ADSC) in reducing the necrosis area in an experimental model of cutaneous ischemic flap in rats submitted to subcutaneous nicotine injection to simulate a smoker patient. Methods In an experimental study, 30 rats were enrolled and divided into two experimental groups of 15 animals all submitted to a subcutaneous nicotine injection to create ischemic cutaneous flaps on their backs. Other 10 animals were used only to obtain adipose tissue derived stem cells (ADSC). The first group (n=15) received ADSC treatment at the end of surgery while the other group, the control (n=15), received no other interventions. After euthanasia, a decal was performed on the whole area of the flap, accurately defining the transition from necrosis to healthy region. Photos of all animals were collected and evaluated by scales standardized by Paint-Autocad- 2015 software to define the area of flap necrosis in each rat. Student T test was performed to compare the groups, considering a p< 0.05 significant. Data were analyzed using SPSS IBM ® 18 version. Results Through the analysis of the images by the program Paint-Autocad-2015 and the area of decal obtained by the transparent sheet, we obtained a mean of 46% necrosis of the total area of the flap in the treatment group and 69.4% in the control group. In the descriptive analysis, a mean of 3.7 cm of necrosis CI 95% (3.2 - 4.2) was evident in the treatment group whereas a mean value of 5.56 CI 95% (5.2 - 5.9) was found in control group, with p value <0.001 for this comparison. Conclusion The application of adipose-derived stem cells reduces the percentage of necrosis in an experimental model of randomized cutaneous flap in rats submitted to subcutaneous nicotine injection.

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Ação da l-arginina na evolução de retalhos cutâneos de ratos sob exposição à nicotina

Cited by 9 publications

References 19 publications

Effect of Omega-3 Fatty Acid in the Healing Process of Colonic Anastomosis in Rats

Effect of Omega-3 Fatty Acid in the Healing Process of Colonic Anastomosis in Rats

Avaliação da Exposição e da Interrupção da Nicotina Durante a Cicatrização em Ratos Wistar

Use of derived adipose stem cells to reduce complications of cutaneous scarring in smokers. An experimental model in rats

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