Ação de extrato e óleo de nim no controle de Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Acari: Ixodidae) em laboratório

Broglio-Micheletti, Sônia Maria Forti; Dias, Nívia da Silva; Valente, Ellen Carine Neves; Souza, Leilianne Alves de; Lopes, Diego Olympio Peixoto; Santos, Jakeline Maria dos

doi:10.1590/s1984-29612010000100009

Cited by 12 publications

(3 citation statements)

References 10 publications

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“…In ticks, the use of neem products showed relevant effects for the control of Amblyomma variegatum (Ndumu et al 1999), Hyalomma dromedarii (Al-Rajhy et al 2003), Hyalomma anatolicum excavatum (Abdel-Shafy and Zayed, 2002), Rhipicephalus decoloratus (Choudhury 2009), Rhipicephalus (Boophilus) microplus (Srivastava et al 2008;Broglio-Micheletti et al 2010) and R. sanguineus (Denardi et al 2010(Denardi et al , 2011(Denardi et al , 2012, affecting reproductive processes or even causing mortality.…”

Section: Introductionmentioning

confidence: 99%

Morphological effects of neem (Azadirachta indica A. Juss) seed oil with known azadirachtin concentrations on the oocytes of semi-engorged Rhipicephalus sanguineus ticks (Acari: Ixodidae)

et al. 2014

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The concern about the harmful effects caused by synthetic pesticides has led to the search for safe and ecological alternatives for pest control. In this context, the neem tree (Azadirachta indica) stands out due to its repellent properties and effects on various arthropods, including ticks. For this reason, this study aimed to demonstrate the potential of neem as a control method for Rhipicephalus sanguineus ticks, important vectors of diseases in the veterinary point of view. For this, R. sanguineus semi-engorged females were subjected to treatment with neem seed oil enriched with azadirachtin, its main compound, and ovaries were assessed by means of morphological techniques in conventional light microscopy, confocal laser scanning microscopy, and transmission electron microscopy. Neem demonstrated a clear dose-dependent effect in the analyzed samples. The observed oocytes presented, especially in the groups treated with higher concentrations of neem oil, obvious signs of cytoplasmic disorganization, cellular vacuolization, nuclear and nucleolar irregularity, dilation in mitochondrial cristae, alterations in mitochondrial matrix, and swelling of rough endoplasmic reticulum. Intracellular microorganisms were observed in all analyzed groups, reinforcing the importance of ticks in the transmission of pathogens. A greater quantity of microorganisms was noted as the concentration of neem increased, indicating that the damaged oocytes may be more susceptible for their development. Such morphological alterations may promote future damages in reproductive performance of these animals and demonstrate the potential of neem seed oil for the control of R. sanguineus ticks, paving the way for new, cheaper, and safer methods of control.

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Section: Introductionmentioning

confidence: 99%

Morphological effects of neem (Azadirachta indica A. Juss) seed oil with known azadirachtin concentrations on the oocytes of semi-engorged Rhipicephalus sanguineus ticks (Acari: Ixodidae)

et al. 2014

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“…The effects of neem active ingredients on ticks have been demonstrated in studies on Amblyomma variegatum (Ndumu, George, & Choudhur, 1999) Broglio-Micheletti et al, 2010;Srivastava, Ghosh, & Mandal, 2008) and R. sanguineus (Denardi et al, 2010(Denardi et al, , 2011(Denardi et al, , 2012, and the chemical was proven to affect the reproductive processes and the mortality rate in these species.…”

mentioning

confidence: 97%

The effects of neem oil (Azadirachta indica A. JUSS) enriched with different concentrations of azadirachtin on the integument of semi‐engorged Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) females

Souza

Remédio

Arnosti

et al. 2017

Microscopy Res & Technique

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Several studies searching for methods to control Rhipicephalus sanguineus s.l., (dog tick) infestations have been developed aiming to minimize the damages caused by these ectoparasites to the hosts and the environment, which is harmed by the indiscriminate use of toxic acaricide products. In this scenario, neem oil has been used as a natural alternative against ticks, once this chemical has repellent properties and interferes in the growth regulation of these ectoparasites, inhibiting ecdysis. The present study evaluated the effects of azadirachtin-enriched neem oil on the integument of semi-engorged R.sanguineus s.l., females through morphohistological techniques. The results showed the occurrence of significant morphological and histochemical alterations, mainly in the females exposed to higher concentrations, which demonstrates the dose-dependent action of the chemical. A decrease in the cuticle thickness was observed, as well as a modification in the distribution of the epithelial cells, which displayed pyknotic and fragmented nuclei, and intensely vacuolated cytoplasm, indicating that these cells would be undergoing death processes. These morphological alterations observed in the integument of the females exposed to the azadirachtin-enriched neem oil encourage the use of this chemical as a strategy to control these ectoparasites.

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“…As espécies de bambu costumam ser empregadas para a geração de energia, alimentação (humana e animal) e construção artesanal e industrial (BALDUINO JÚNIOR et al, 2016), além de serem apontadas como capazes de conservar ou melhorar a fertilidade do solo devido à constante cobertura (SANTOS, 2014). De forma semelhante, o nim indiano apresenta inúmeras potencialidades de uso, com destaque para a extração do óleo de suas sementes, que possui efeito herbicida (RICKLI et al, 2011), fungicida (RAZA et al, 2016), inseticida (MURUGAN et al, 2016), bactericida (GALEANE et al, 2017) e carrapaticida (BROGLIO-MICHELETTI et al, 2010), além de diversos benefícios à saúde humana (KUMAR et al, 2015).…”

Section: Introductionunclassified

Decomposição da serapilheira foliar em plantios de bambu, nim indiano e eucalipto

et al. 2020

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O processo de decomposição da serapilheira regula o acúmulo de matéria orgânica no solo e a ciclagem de nutrientes, desempenhando importante papel na manutenção de ecossistemas florestais. Objetivou-se avaliar a decomposição da fração foliar da serapilheira de plantios homogêneos de Bambusa vulgaris, Azadirachta indica e de Eucalyptus urophylla, utilizando como referência uma área de Floresta Estacional Semidecidual. As áreas de estudo estão localizadas no município de Vitória da Conquista, Bahia, Brasil. Para a avaliação da decomposição utilizou-se o método dos litterbags, sendo as coletas realizadas aos 30, 75, 120, 180, 240 e 350 dias. A partir dos resultados de massa remanescente, estimou-se a constante de decomposição (k) e o tempo de meia-vida. A serapilheira foliar foi submetida à análises químicas para determinação dos teores de carbono, nitrogênio, polifenóis, celulose e lignina. Ao final de um ano, bambu e nim indiano tiveram mais de 65% da sua serapilheira decomposta, com constantes k iguais a 0,0033 e 0,0069 g g-1 dia, respectivamente. O plantio de eucalipto e a floresta nativa apresentaram perdas de massa inferiores a 50% e valores de k menores que 0,0020 g g-1 dia. A serapilheira foliar do bambu e do nim indiano mostra-se mais propensa à decomposição, com taxas superiores ao eucalipto e à floresta nativa, o que proporciona a estas duas espécies uma maior capacidade de reciclar matéria orgânica e nutrientes.

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Ação de extrato e óleo de nim no controle de Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Acari: Ixodidae) em laboratório

Cited by 12 publications

References 10 publications

Morphological effects of neem (Azadirachta indica A. Juss) seed oil with known azadirachtin concentrations on the oocytes of semi-engorged Rhipicephalus sanguineus ticks (Acari: Ixodidae)

Morphological effects of neem (Azadirachta indica A. Juss) seed oil with known azadirachtin concentrations on the oocytes of semi-engorged Rhipicephalus sanguineus ticks (Acari: Ixodidae)

The effects of neem oil (Azadirachta indica A. JUSS) enriched with different concentrations of azadirachtin on the integument of semi‐engorged Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) females

Decomposição da serapilheira foliar em plantios de bambu, nim indiano e eucalipto

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