Acaricidal and insecticidal activity of essential oils on Varroa destructor (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae)

Damiáni, Natalia; Gende, Liesel Brenda; Bailac, Pedro N.; Marcangeli, Jorge; Eguaras, Martín Javier

doi:10.1007/s00436-009-1639-y

Cited by 82 publications

(55 citation statements)

References 24 publications

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“…They have been reported to have antibacterial, anti-inflammatory, antioxidant, and vasorelaxant effects (Baser, 2008;Peixoto-Neves et al, 2010). In addition, both carvacrol and thymol are used as acaricides to control mite (Varroa destructor) infestation of honeybee hives (Damiani et al, 2009). There is evidence that carvacrol and thymol are positive allosteric modulators of both invertebrate and mammalian GABA A Rs (Priestley et al, 2003;García et al, 2006;Reiner et al, 2013).…”

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confidence: 99%

Activation of Human 5-Hydroxytryptamine Type 3 Receptors via an Allosteric Transmembrane Site

et al. 2014

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In common with other members of the Cys-loop family of pentameric ligand-gated ion channels, 5-hydroxytryptamine type 3 receptors (5-HT 3 Rs) are activated by the binding of a neurotransmitter to an extracellular orthosteric site, located at the interface of two adjacent receptor subunits. In addition, a variety of compounds have been identified that modulate agonistevoked responses of 5-HT 3 Rs, and other Cys-loop receptors, by binding to distinct allosteric sites. In this study, we examined the pharmacological effects of a group of monoterpene compounds on recombinant 5-HT 3 Rs expressed in Xenopus oocytes. Two phenolic monoterpenes (carvacrol and thymol) display allosteric agonist activity on human homomeric 5-HT 3A Rs (64 6 7% and 80 6 4% of the maximum response evoked by the endogenous orthosteric agonist 5-HT, respectively). In addition, at lower concentrations, where agonist effects are less apparent, carvacrol and thymol act as potentiators of responses evoked by submaximal concentrations of 5-HT. By contrast, carvacrol and thymol have no agonist or potentiating activity on the closely related mouse 5-HT 3A Rs. Using subunit chimeras containing regions of the human and mouse 5-HT3A subunits, and by use of site-directed mutagenesis, we have identified transmembrane amino acids that either abolish the agonist activity of carvacrol and thymol on human 5-HT 3A Rs or are able to confer this property on mouse 5-HT 3A Rs. By contrast, these mutations have no significant effect on orthosteric activation of 5-HT 3A Rs by 5-HT. We conclude that 5-HT 3A Rs can be activated by the binding of ligands to an allosteric transmembrane site, a conclusion that is supported by computer docking studies.

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confidence: 99%

Activation of Human 5-Hydroxytryptamine Type 3 Receptors via an Allosteric Transmembrane Site

et al. 2014

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“…In addition, it is classified as non-toxic veterinary drug according to EU regulations without requiring maximal residue level in honey products (Imdorf et al, 1999). Damiani et al (2009) studied the acaricide activity of essential oils of Thymus vulgaris, Laurus nobilis, Lavandula officinalis and Lavandula hybrida containing linalool, 1,8-Cineole and thymol as the main compounds in their composition against V. destructor. All essential oils caused mite mortality without severe harmful effects on adult bees.…”

Section: Essential Oils Against Honey Bee Mitesmentioning

confidence: 99%

Plant Essential Oils Used Against Some Bee Diseases

Tutun

Koç

Kart

2018

Turkish JAF Sci.Tech.

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The most common honey bee diseases are American foulbrood (AFB) caused by the bacterium Paenibacillus larvae, Chalkbrood caused by fungus Ascosphaera apis and diseases caused by parasitic mites such as Acarapis woodi, Varroa destructor. These diseases and pests not only cause economic loss but also cause ecological problems related to the role of honey bees, as the most important pollinators on Earth. Synthetic acaricides and antibiotics are used to keep the diseases and mites in control. Use of the drugs lead to the development of drug-resistant organisms, detrimental effect on non-target organisms and the residue problem in bee products. For this reasons, the need for alternative control methods has become compulsory in recent years. It has been known that some plant oils used widely in perfumery and food industry for flavor and smell have been used as repellent to certain insects, bactericide and fungicide. Therefore, intensive studies have been carried out on plants with anti-mites, antibacterial and antifungal potentials and these studies are still going on. Recently, studies in this area have shown that essential oils of plants such as thyme, cloves, mint, lemon grass, cinnamon, grapefruit, rosemary, marigold, are lethal to some mites, bacteria and fungi. In addition, it has been reported that some components, isolated from these plants such as sanguinarine, thymoquinone, capsaicin, carvacrol, citral, eugenol, thymol, show these effects on the organisms. As a result, in countries rich in biodiversity due to endemic plant species, the essential oils used in control of these diseases should be favored instead of or in combination with conventional drugs in integrated the disease management programs because of the lack of harmful effects of essential oils on non-target organisms and environment.

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“…destructor switched from its native host from Apis cerana to Apis mellifera 4 decades ago, later spreading to nearly all parts of the world (Sammataro et al, 2000) and becoming the greatest threat to A. mellifera health worldwide. The need for regular mite control treatments (typically chemical acaricides) to control the parasite population in western honeybee apiculture efforts (Annoscia et al, 2012) resulted in the rapid evolution of mite resistance against these chemicals, decreasing their efficiency (Damiani et al, 2009;Kanga et al, 2010). These control treatments often contaminate apicultural products (honey, pollen) with acaricide residues (Karazafiris et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor

Ji¹,

Yin²,

Liu³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The Varroa destructor mite has become the greatest threat to Apis mellifera health worldwide, but rarely causes serious damage to its native host Apis cerana. Understanding the resistance mechanisms of eastern bees against Varroa mites will help researchers determine how to protect other species from this organism. The A. cerana genome has not been previously sequenced; hence, here we sequenced the A. cerana nurse workers transcriptome and monitored the differential gene expression of A. cerana bees challenged by V. destructor. Using de novo transcriptome assembly, we obtained 91,172 unigenes (transcripts) for A. cerana. Differences in gene expression levels between the unchallenged (Con) and challenged (Con2) samples were estimated, and a total of 36,691 transcripts showed a 2-fold difference (at least) 8889©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 8888-8900 (2014) DEGs in Apis cerana infected with Varroa between the 2 libraries. A total of 272 differentially expressed genes showed differences greater than 15-fold, and 265 unigenes were present at higher levels in Con2 than in Con. Among the upregulated unigenes in the Con2 colony, genes related to skeletal muscle movement (troponin and calcium-transporting ATPase), olfactory sensitivity (odorant binding proteins, and Down syndrome cell adhesion molecule gene) and transcription factors (cyclic adenosine monophosphate-responsive element-binding protein and transcription factor mblk-1) appeared to be involved in Varroa resistance. Real-time polymerase chain reaction was performed to validate these differentially expressed genes screened by the sequencing approach, and sufficient consistency was observed between the two methods. These findings strongly support that hygienic and grooming behaviors play important roles in Varroa resistance.

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Acaricidal and insecticidal activity of essential oils on Varroa destructor (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae)

Cited by 82 publications

References 24 publications

Activation of Human 5-Hydroxytryptamine Type 3 Receptors via an Allosteric Transmembrane Site

Activation of Human 5-Hydroxytryptamine Type 3 Receptors via an Allosteric Transmembrane Site

Plant Essential Oils Used Against Some Bee Diseases

Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor

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