Acaricidal effect of cell-free supernatants from Xenorhabdus and Photorhabdus bacteria against Tetranychus urticae (Acari: Tetranychidae)

Eroglu, Ceren; Çimen, Harun; Uluğ, Derya; Karagöz, Mehmet Ali; Hazır, Selçuk; Çakmak, İbrahim

doi:10.1016/j.jip.2018.12.004

Cited by 41 publications

(29 citation statements)

References 37 publications

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“…Furthermore, the identification of fabclavine derivatives described here might support recent studies that revealed Xenorhabdus and Photorhabdus strains having ascaricidal or larvicidal activity. Here, especially X. szentirmaii-, X. indica-, X. stockiae-, as well as X. stockiae-related isolates showed the best activity [18,19,47]. Although these strains were confirmed as fabclavine producers in our current study, future work is required to confirm fabclavines as the active compounds here as well (Table 3) [20].…”

Section: Resultsmentioning

confidence: 56%

Fabclavine diversity in Xenorhabdus bacteria

Wenski

Çimen

Berghaus

et al. 2020

Beilstein J. Org. Chem.

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The global threat of multiresistant pathogens has to be answered by the development of novel antibiotics. Established antibiotic applications are often based on so-called secondary or specialized metabolites (SMs), identified in large screening approaches. To continue this successful strategy, new sources for bioactive compounds are required, such as the bacterial genera Xenorhabdus or Photorhabdus. In these strains, fabclavines are widely distributed SMs with a broad-spectrum bioactivity. Fabclavines are hybrid SMs derived from nonribosomal peptide synthetases (NRPS), polyunsaturated fatty acid (PUFA), and polyketide synthases (PKS). Selected Xenorhabdus and Photorhabdus mutant strains were generated applying a chemically inducible promoter in front of the suggested fabclavine (fcl) biosynthesis gene cluster (BGC), followed by the analysis of the occurring fabclavines. Subsequently, known and unknown derivatives were identified and confirmed by MALDI–MS and MALDI–MS2 experiments in combination with an optimized sample preparation. This led to a total number of 22 novel fabclavine derivatives in eight strains, increasing the overall number of fabclavines to 32. Together with the identification of fabclavines as major antibiotics in several entomopathogenic strains, our work lays the foundation for the rapid fabclavine identification and dereplication as the basis for future work of this widespread and bioactive SM class.

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Section: Resultsmentioning

confidence: 56%

Fabclavine diversity in Xenorhabdus bacteria

Wenski

Çimen

Berghaus

et al. 2020

Beilstein J. Org. Chem.

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“…To select the effective range of the experimental variables (Table 2), preliminary experiments were conducted with a broad concentration range of NaCl (0.5-10 g/L) and C/N (4.53-30), which were individually supplemented to WS4. Our findings shrink these ranges to (2-6 g/L) and (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) shown in Table 2. Besides, using two inocula grown differently, in LB and WS4, the same growth rate and the same biomass production were obtained.…”

Section: C/n Ratio As Shown Inmentioning

confidence: 46%

“…100% mortality of this insect larvae was reached after oral administration of broth medium at a concentration of 12 × 10 8 cells/mL 9 . Moreover, mortality of protonymph, deutonymh, adult males and adult females of the polyphagous pest Tetranychus urticae, belonging to the order of Trombidiformes was recorded after oral administration of P. temperata cell free supernatant 10 . Diptera is also an insect order susceptible to Photorhabdus toxins.…”

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confidence: 99%

Development of a cost-effective medium for Photorhabdus temperata bioinsecticide production from wastewater and exploration of performance kinetic

Keskes

Jallouli

Atitallah

et al. 2021

Sci Rep

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This study investigates the optimization of the culture conditions for enhancing Photorhabdus temperata biopesticide production using wastewater (WS4) as a raw material. Box-Behnken design (BBD) was used to evaluate the effects of carbon to nitrogen ratio (C/N), sodium chloride concentration and inoculum size on P. temperata biomass production and insecticidal activity. For an enhanced biopesticide production, the optimum operating conditions were as follows: inoculum size = 4%; C/N ratio = 12.5 and [NaCl] = 4 g/L for two responses. 1.95 and 2.75 fold improvements in oral toxicity and biomass production were respectively obtained in the cost-effective medium developed in this study (WS4 I) using the three variables at their optimal values. Under the optimized conditions, WS4 I-grown cells exhibited higher membrane integrity according to flow cytometry analysis since dead cells presented only 9.2% compared to 29.2% in WS4. From batch fermentations carried out in WS4 I and WS4, P. temperata kinetic parameters in terms of biomass production and substrate consumption rates were modeled. The obtained results showed that the maximum specific growth rate in WS4 I was of 0.43 h−1 while that obtained in WS4 was of 0.14 h−1. In addition, the efficiency of P. temperata to metabolize organic carbon was enhanced by optimizing the culture conditions. It reached 72.66% instead of 46.18% in the control fermentation after 10 h of incubation. Under the optimized conditions, P. temperata cells showed the highest specific consumption rate resulting in a toxin synthesis improvement.

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“…100 % mortality of this insect larvae was reached after oral administration of broth medium at a concentration of 12×10 8 cells/mL (Jallouli et al 2013). Moreover, mortality of protonymph, deutonymh, adult males and adult females of the polyphagous pest Tetranychus urticae, belonging to the order of Trombidiformes was recorded after oral administration of P. temperata cell free supernatant (Eroglu et al 2019). Diptera is another insect order susceptible to Photorhabdus toxins.…”

Section: Introductionmentioning

confidence: 93%

Development of a Cost-effective Medium for Photorhabdus Temperata Bioinsecticide Production From Wastewater and Exploration of Performance Kinetic

Keskes

Jallouli

Atitallah

et al. 2020

Preprint

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This study investigates the optimization of culture conditions for enhancing Photorhabdus temperata biopesticide production using wastewater (WS4) as a raw material. Box-Behnken design (BBD) was used to evaluate the effect of carbon to nitrogen ratio (C/N), NaCl concentration and inoculum size on Photorhabdus temperata biomass production and insecticidal activity. Modelling results suggest that the selected variables had contributed significantly to the responses. For enhanced biopesticide production the optimum operation conditions were as follow: inoculum size=4 %; C/N ratio=12.5 and sodium chloride concentration= 4 g/L for two responses. 1.95 and 2.75 folds improvement in oral toxicity and biomass production, respectively, were obtained when using the three variables at their optimum values. From batch fermentations carried out in the cost-effective medium developed in this study (WS4 I) and WS4 used as control, P. temperata kinetic parameters in term of biomass production and substrate consumption were modeled. The obtained results showed that the maximum specific growth rate was of 0.38 h-1 compared to 0.16 h-1 obtained in WS4. In addition, the efficiency of P. temperata to metabolize organic carbon was enhanced by optimizing culture conditions reaching 70 % instead of 47.2 % in the control fermentation. Under the optimized conditions, P. temperata cells showed higher specific consumption rate resulting in P. temperata toxin synthesis improvement.

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Acaricidal effect of cell-free supernatants from Xenorhabdus and Photorhabdus bacteria against Tetranychus urticae (Acari: Tetranychidae)

Cited by 41 publications

References 37 publications

Fabclavine diversity in Xenorhabdus bacteria

Fabclavine diversity in Xenorhabdus bacteria

Development of a cost-effective medium for Photorhabdus temperata bioinsecticide production from wastewater and exploration of performance kinetic

Development of a Cost-effective Medium for Photorhabdus Temperata Bioinsecticide Production From Wastewater and Exploration of Performance Kinetic

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